FastQCFastQC Report
Thu 9 Feb 2017
SRR2727588.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727588.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9589938
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT835180.8708919703130511No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT826080.8614028578704055No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT538680.561713746220257No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT349200.3641316554914119No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120060.12519371866637719No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT96910.10105383371613039No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA334700.015.4820741
TCGTTAC13850.011.62093823
TTAATCC32850.011.5449024
GTATCAA451500.011.4564792
TAATCCC31250.011.1296015
CCTTTAA42000.010.8357151
TAATACT21000.010.747624
ACGAGAC12100.010.7024822
GTATACG3503.45608E-1110.5714291
CGCGTAA1400.001864053910.57142810
AACGCAG518500.010.5148515
CGTTACG11950.010.37238524
TATACAC18050.010.3518013
TTTAATC40000.010.1753
TCTATAC8550.010.1695913
TAGATCG5150.010.0582535
GGGTAAG48250.010.0455961
GTTACGG20950.09.89021525
TACACAG32550.09.88940055
TATACTG13850.09.8844765
Produced by FastQC (version 0.11.5)