##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727588.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9589938
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.27087098998972	33.0	31.0	34.0	31.0	34.0
2	32.49024613089261	34.0	31.0	34.0	31.0	34.0
3	32.55286082141511	34.0	31.0	34.0	31.0	34.0
4	36.08220334688295	37.0	35.0	37.0	35.0	37.0
5	36.003598771962864	37.0	35.0	37.0	35.0	37.0
6	36.02177094367033	37.0	35.0	37.0	35.0	37.0
7	35.95623256375589	37.0	35.0	37.0	35.0	37.0
8	35.96006668656252	37.0	35.0	37.0	35.0	37.0
9	37.63046872670084	39.0	37.0	39.0	35.0	39.0
10	37.58180782816323	39.0	37.0	39.0	35.0	39.0
11	37.62060484645469	39.0	37.0	39.0	35.0	39.0
12	37.50874228801062	39.0	37.0	39.0	35.0	39.0
13	37.50110469952986	39.0	37.0	39.0	35.0	39.0
14	38.739091222487566	40.0	38.0	41.0	35.0	41.0
15	38.72632909618394	40.0	38.0	41.0	34.0	41.0
16	38.646790521482	40.0	38.0	41.0	34.0	41.0
17	38.624074941881794	40.0	38.0	41.0	34.0	41.0
18	38.56032604173249	40.0	38.0	41.0	34.0	41.0
19	38.551331718724356	40.0	38.0	41.0	34.0	41.0
20	38.451970492405685	40.0	38.0	41.0	34.0	41.0
21	38.36237106016744	40.0	38.0	41.0	34.0	41.0
22	38.26170992972009	40.0	38.0	41.0	33.0	41.0
23	38.43418560161703	40.0	38.0	41.0	34.0	41.0
24	38.430174835332615	40.0	38.0	41.0	34.0	41.0
25	38.42121075235314	40.0	38.0	41.0	34.0	41.0
26	38.282043950649104	40.0	38.0	41.0	34.0	41.0
27	38.298817990272724	40.0	38.0	41.0	34.0	41.0
28	38.19087328823189	40.0	38.0	41.0	33.0	41.0
29	38.15294123903617	40.0	38.0	41.0	33.0	41.0
30	38.06886374030781	40.0	37.0	41.0	33.0	41.0
31	37.93913203609867	40.0	37.0	41.0	33.0	41.0
32	37.80905246728394	40.0	37.0	41.0	33.0	41.0
33	37.697094079231796	40.0	37.0	41.0	33.0	41.0
34	37.605713926409116	40.0	37.0	41.0	33.0	41.0
35	37.490719126651285	40.0	36.0	41.0	32.0	41.0
36	37.38251217056878	40.0	36.0	41.0	32.0	41.0
37	37.27713463840955	40.0	36.0	41.0	31.0	41.0
38	37.137834676303434	40.0	36.0	41.0	31.0	41.0
39	37.00440513796857	40.0	35.0	41.0	31.0	41.0
40	36.87406957166981	40.0	35.0	41.0	30.0	41.0
41	36.72369226996045	40.0	35.0	41.0	30.0	41.0
42	36.57188325930783	39.0	35.0	41.0	30.0	41.0
43	36.38675432521045	39.0	35.0	41.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	4.0
11	6.0
12	18.0
13	31.0
14	34.0
15	72.0
16	151.0
17	281.0
18	483.0
19	1033.0
20	1903.0
21	3673.0
22	6808.0
23	11188.0
24	18296.0
25	28364.0
26	41318.0
27	59114.0
28	82395.0
29	110555.0
30	145100.0
31	187837.0
32	238891.0
33	308545.0
34	407398.0
35	533922.0
36	739794.0
37	1181526.0
38	1873882.0
39	3607315.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.96948530845559	19.03128049420132	12.79135485547456	25.207879341868527
2	19.344827881056165	22.35078057856057	33.622636559277026	24.681754981106238
3	20.071214224742643	23.211547353069438	30.785725622000893	25.93151280018703
4	15.005529754206961	16.282805999371426	33.50959099005645	35.20207325636516
5	14.757196553304098	36.73045644299264	33.414095065056735	15.098251938646529
6	34.35402814908709	34.89730590541878	14.47228334531464	16.276382600179478
7	28.80850741683627	31.178981553373962	20.63890298352294	19.373608046266828
8	27.67792659347745	34.22375619112449	18.945450950777783	19.152866264620272
9	26.82048622212156	14.269351897791205	19.56058527177131	39.34957660831592
10	17.296785443242698	27.838021476259804	32.294692624707274	22.570500455790224
11	34.78142403006151	22.535693140039072	20.998008537698574	21.684874292200846
12	21.530785704766807	26.371526072431333	29.49648892412026	22.6011992986816
13	31.320004362906207	20.330506829136954	24.283201830919033	24.066286977037805
14	22.00687845948535	21.705093401020946	26.58244505856034	29.70558308093337
15	25.139849704972022	28.192361619021938	23.347783895995992	23.32000478001005
16	23.36075582553297	26.143484973521204	26.010898089226437	24.48486111171939
17	23.246803055452496	26.567033071538106	26.543988084177393	23.642175788832002
18	23.54101767915496	25.0938744338076	27.42108447416448	23.94402341287295
19	24.46629999067773	25.034603977627384	28.34151795350502	22.157578078189868
20	23.786420725556308	24.860932364734786	28.34513632934853	23.007510580360375
21	23.342830787852854	25.39703593495599	28.518380410801402	22.741752866389753
22	23.561925009317058	25.043300592767125	27.853277049340676	23.54149734857514
23	23.0984600734645	25.246597006153742	28.08145370699998	23.573489213381777
24	22.931065873418575	25.44565981552748	28.238117910668453	23.385156400385487
25	23.43483346816215	25.00429095579137	28.03816875562699	23.522706820419486
26	22.909042790474764	25.755150867503	28.278858528595286	23.056947813426948
27	23.450422724317928	25.71445195996053	28.072611105514966	22.76251421020657
28	22.94412122372428	25.976403601358005	27.898053146954656	23.18142202796306
29	22.9104817987353	26.181243298966063	28.12278869790399	22.785486204394648
30	23.25833597672894	25.800583903670706	27.86910614020654	23.07197397939382
31	23.164425046335026	25.489059470457477	28.23820133143718	23.108314151770323
32	22.78260818787358	26.07265031327627	27.837249834149087	23.307491664701065
33	22.66260741206043	25.76629796772409	28.072256567247877	23.4988380529676
34	23.255155559921242	25.91817590478687	28.049931084017437	22.77673745127445
35	23.013131054653325	26.29107716859066	27.804924286267546	22.890867490488468
36	22.976060950550462	26.171420503448513	27.869596237222805	22.98292230877822
37	23.01786518327856	26.13559128328046	27.797176582372064	23.049366951068922
38	22.95729127758699	26.265341861438518	27.91010744803564	22.867259412938854
39	22.702096718456367	26.73683604628101	27.796738623336253	22.764328611926377
40	23.26645907408369	26.30760490839461	27.714277193450055	22.711658824071645
41	22.873870508860435	26.48666758846616	27.60068938923276	23.03877251344065
42	22.910951040559386	26.450473402434927	27.313085861451867	23.32548969555382
43	22.968813771267342	26.208010938131192	27.34518200221941	23.477993288382052
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	4567.0
1	4319.5
2	4072.0
3	6304.0
4	8536.0
5	8536.0
6	10997.0
7	13458.0
8	12190.0
9	10922.0
10	16522.0
11	22122.0
12	22122.0
13	49020.5
14	75919.0
15	111143.0
16	146367.0
17	128828.5
18	111290.0
19	111290.0
20	132476.0
21	153662.0
22	120579.0
23	87496.0
24	101054.0
25	114612.0
26	114612.0
27	136346.5
28	158081.0
29	183502.5
30	208924.0
31	237374.0
32	265824.0
33	265824.0
34	296247.5
35	326671.0
36	362021.5
37	397372.0
38	431193.5
39	465015.0
40	465015.0
41	502013.5
42	539012.0
43	575427.0
44	611842.0
45	639441.5
46	667041.0
47	667041.0
48	705942.5
49	744844.0
50	761692.0
51	778540.0
52	766466.5
53	754393.0
54	754393.0
55	714363.0
56	674333.0
57	624226.0
58	574119.0
59	523519.0
60	472919.0
61	472919.0
62	419394.0
63	365869.0
64	314701.0
65	263533.0
66	225421.0
67	187309.0
68	187309.0
69	159802.0
70	132295.0
71	113563.5
72	94832.0
73	76956.5
74	59081.0
75	59081.0
76	48860.5
77	38640.0
78	31448.0
79	24256.0
80	19065.5
81	13875.0
82	13875.0
83	11009.0
84	8143.0
85	6528.5
86	4914.0
87	3887.5
88	2861.0
89	2861.0
90	2146.0
91	1431.0
92	970.5
93	510.0
94	405.0
95	300.0
96	300.0
97	189.5
98	79.0
99	68.0
100	57.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	9589938.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.181215088283835
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.00785496879196	34.34127243744073
2	12.231324012898703	11.052521620825345
3	4.287286203898547	5.811144003701163
4	2.0539903695094566	3.7120712269628138
5	1.200532404572644	2.7120756395725603
6	0.7650929678645606	2.0740697966173287
7	0.559570189328015	1.7697442756714528
8	0.41414644893321434	1.496931182984041
9	0.3135339383545893	1.274925987564788
>10	1.892157590413714	16.056771087807554
>50	0.16970100715883535	5.312388507346784
>100	0.09738975368378538	8.132547611478126
>500	0.0052250977638415705	1.571356892950113
>1k	0.0020102008917890954	1.6461774172176074
>5k	6.931722626132524E-5	0.24950297521988174
>10k+	1.1552871043554207E-4	2.786499336639784
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	83518	0.8708919703130511	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	82608	0.8614028578704055	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	53868	0.561713746220257	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	34920	0.3641316554914119	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12006	0.12519371866637719	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9691	0.10105383371613039	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.128278827245807E-5	0.0	0.0	2.0855192181638712E-5	1.0427596090819356E-5
2	3.4411067099703876E-4	0.0	0.0	4.1710384363277424E-5	1.0427596090819356E-5
3	5.213798045409678E-4	0.0	0.0	5.213798045409678E-5	1.0427596090819356E-5
4	5.630901889042453E-4	0.0	0.0	9.384836481737421E-5	2.0855192181638712E-5
5	0.00227321594779862	0.0	0.0	1.251311530898323E-4	2.0855192181638712E-5
6	0.0033159755568805556	0.0	0.0	1.981243257255678E-4	3.128278827245807E-5
7	0.0037643621887857876	0.0	0.0	2.189795179072065E-4	5.213798045409678E-5
8	0.004984390931411653	0.0	0.0	2.815450944521226E-4	7.29931726357355E-5
9	0.005411922371135246	0.0	0.0	3.128278827245807E-4	8.342076872655485E-5
10	0.007810269472023698	0.0	0.0	4.275314397235936E-4	8.342076872655485E-5
11	0.00841507004529122	0.0	0.0	4.796694201776904E-4	8.342076872655485E-5
12	0.008884311869378093	0.0	0.0	5.005246123593292E-4	9.384836481737421E-5
13	0.009697664364462002	0.0	0.0	5.83945381085884E-4	9.384836481737421E-5
14	0.010896837914906228	0.0	0.0	6.569385537216195E-4	1.668415374531097E-4
15	0.011897887139624886	1.0427596090819356E-5	0.0	6.986489380848969E-4	1.7726913354392906E-4
16	0.012554825693346506	1.0427596090819356E-5	0.0	7.716421107206324E-4	1.8769672963474843E-4
17	0.012763377615162892	1.0427596090819356E-5	0.0	7.820697068114517E-4	1.981243257255678E-4
18	0.012982357133070098	1.0427596090819356E-5	0.0	7.924973029022712E-4	1.981243257255678E-4
19	0.013482881745429429	1.0427596090819356E-5	0.0	9.07200859901284E-4	2.0855192181638713E-4
20	0.016903133263218177	1.0427596090819356E-5	0.0	0.0011261803778084905	2.0855192181638713E-4
21	0.02447356802515303	1.0427596090819356E-5	0.0	0.001699698162803555	2.189795179072065E-4
22	0.02495323744533072	2.0855192181638712E-5	0.0	0.002085519218163871	2.189795179072065E-4
23	0.02543290686550841	2.0855192181638712E-5	0.0	0.002314926332161897	2.91972690542942E-4
24	0.026371390513682154	2.0855192181638712E-5	0.0	0.0027007473875222134	2.91972690542942E-4
25	0.027007473875222135	2.0855192181638712E-5	0.0	0.002961437289792697	3.0240028663376135E-4
26	0.030427725393010883	2.0855192181638712E-5	0.0	0.0036392310356959555	3.0240028663376135E-4
27	0.03132449865682135	2.0855192181638712E-5	0.0	0.005391067178953607	3.0240028663376135E-4
28	0.03269051374471868	2.0855192181638712E-5	0.0	0.010458878879091815	3.128278827245807E-4
29	0.03291992085871671	2.0855192181638712E-5	0.0	0.02022953641618955	3.128278827245807E-4
30	0.0333995902788944	3.128278827245807E-5	0.0	0.03300334162744326	3.128278827245807E-4
31	0.034254653158341586	3.128278827245807E-5	0.0	0.0616792308771965	3.128278827245807E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	33470	0.0	15.482074	1
TCGTTAC	1385	0.0	11.620938	23
TTAATCC	3285	0.0	11.544902	4
GTATCAA	45150	0.0	11.456479	2
TAATCCC	3125	0.0	11.129601	5
CCTTTAA	4200	0.0	10.835715	1
TAATACT	2100	0.0	10.74762	4
ACGAGAC	1210	0.0	10.70248	22
GTATACG	350	3.45608E-11	10.571429	1
CGCGTAA	140	0.0018640539	10.571428	10
AACGCAG	51850	0.0	10.514851	5
CGTTACG	1195	0.0	10.372385	24
TATACAC	1805	0.0	10.351801	3
TTTAATC	4000	0.0	10.175	3
TCTATAC	855	0.0	10.169591	3
TAGATCG	515	0.0	10.058253	5
GGGTAAG	4825	0.0	10.045596	1
GTTACGG	2095	0.0	9.890215	25
TACACAG	3255	0.0	9.8894005	5
TATACTG	1385	0.0	9.884476	5
>>END_MODULE