FastQCFastQC Report
Thu 9 Feb 2017
SRR2727587.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727587.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8083649
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT763020.9439054070754432No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT754200.9329944929573265No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT487910.603576429407066No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT363570.4497597557736611No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT132440.16383690088473657No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT96140.11893143801765761No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92730.11471304605135627No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA298500.015.9155781
TAATCCC44300.015.0756215
GGCGGGT8750.015.01142933
TTAATCC46450.014.9354144
TTTAATC53100.013.692093
CCTTTAA58550.013.36551
GCGGGTT9600.013.10416734
ACGAGAC11300.013.09734522
CTTTAAT59800.012.6530092
GCACTCG32500.012.52307713
GAGACTA11250.012.16888924
CGAGACT12200.012.13114723
CACGAGA11750.012.123404521
ACGGATG27100.011.94649528
CACTCGG33450.011.83557514
GACTAGG12050.011.82157726
TACGGAT26500.011.72830227
GTATCAA407400.011.6975952
ATCTCCG13100.011.580152510
TAGGCAT12450.011.44176729
Produced by FastQC (version 0.11.5)