FastQCFastQC Report
Thu 9 Feb 2017
SRR2727586.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727586.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6078522
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT513250.8443664430267752No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT475200.7817689892378443No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT320760.527694067735545No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT228450.3758314932478652No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGT170560.2805945261035495No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79620.13098578897962368No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACAC34000.023.9411773
ACCGTAC30700.023.92345227
GACCGTA30850.023.8071326
CGTACTA31150.023.39967729
ATCTCCG32350.023.3894910
CTTATAC34100.022.8944281
GGTATCA139050.022.458111
GTACTAG32850.022.30136930
CGAGACC33300.022.22222123
TTATACA36250.022.1489682
ACGAGAC34450.021.42670422
CCCACGA34800.021.4238519
CTAGATC34350.021.38136933
TCTCCGA35800.021.29050311
GCCCACG35400.021.16525718
GAGACCG34650.021.14285924
CCACGAG35750.020.90629220
CACGAGA35900.020.81894121
AGACCGT35200.020.75994325
TACACAT41400.020.4214975
Produced by FastQC (version 0.11.5)