FastQCFastQC Report
Thu 9 Feb 2017
SRR2727585.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727585.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9145813
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT392690.4293658748544279No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT385740.4217667691215642No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT261370.28578104538109406No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT153120.1674208733548346No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA178700.014.6281471
TATACCG3750.013.325
TAATCCC29850.012.5192625
TTAATCC32200.012.1801244
TCGTTAC14400.011.69097223
TTTAATC37450.011.3618163
TAGATCG3700.011.05
GTATCAA239800.010.8932442
CCTTTAA41200.010.7317961
TAGACTG12200.010.4631155
AACGCAG273950.010.45373255
ATACCGT4650.010.3440866
CTCGTTA16900.010.2899422
TCTATAC8600.010.1104653
GTATACG2952.8556315E-810.0338981
CGTTACG11500.09.97391324
CTTTAAT44400.09.9583332
GGCGGGT8200.09.70121933
TCTACAC17100.09.5204683
CGCGTAA1750.00109939579.51428510
Produced by FastQC (version 0.11.5)