##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727585.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9145813
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.983413940346253	31.0	31.0	34.0	30.0	34.0
2	32.169792450381394	33.0	31.0	34.0	30.0	34.0
3	32.24997712067806	34.0	31.0	34.0	30.0	34.0
4	35.887417663142685	37.0	35.0	37.0	35.0	37.0
5	35.77825470518586	37.0	35.0	37.0	35.0	37.0
6	35.816885278542216	37.0	35.0	37.0	35.0	37.0
7	35.73318457309372	37.0	35.0	37.0	33.0	37.0
8	35.71219999796628	37.0	35.0	37.0	33.0	37.0
9	37.38659701439336	39.0	37.0	39.0	34.0	39.0
10	37.2651566350635	39.0	37.0	39.0	34.0	39.0
11	37.3300584650047	39.0	37.0	39.0	34.0	39.0
12	37.18097450713239	39.0	37.0	39.0	33.0	39.0
13	37.217898179199594	39.0	37.0	39.0	33.0	39.0
14	38.40742654589592	40.0	38.0	41.0	33.0	41.0
15	38.40629498984946	40.0	38.0	41.0	33.0	41.0
16	38.33919696368163	40.0	38.0	41.0	33.0	41.0
17	38.292816177194965	40.0	38.0	41.0	33.0	41.0
18	38.21836888639643	40.0	38.0	41.0	33.0	41.0
19	38.215428961864845	40.0	38.0	41.0	33.0	41.0
20	38.139718688759544	40.0	37.0	41.0	33.0	41.0
21	38.04974265273082	40.0	37.0	41.0	33.0	41.0
22	37.969998621227006	40.0	37.0	41.0	33.0	41.0
23	37.868389611727245	40.0	37.0	41.0	32.0	41.0
24	37.77016619517587	40.0	37.0	41.0	32.0	41.0
25	38.00365369377222	40.0	37.0	41.0	33.0	41.0
26	37.96268456396386	40.0	37.0	41.0	33.0	41.0
27	37.92301799741587	40.0	37.0	41.0	33.0	41.0
28	37.867022756752185	40.0	37.0	41.0	33.0	41.0
29	37.80890698290026	40.0	37.0	41.0	32.0	41.0
30	37.72125179030011	40.0	37.0	41.0	32.0	41.0
31	37.62659393976238	40.0	37.0	41.0	32.0	41.0
32	37.53160883564971	40.0	37.0	41.0	32.0	41.0
33	37.45229691444599	40.0	36.0	41.0	31.0	41.0
34	37.36886026425425	40.0	36.0	41.0	31.0	41.0
35	37.26264018299959	40.0	36.0	41.0	31.0	41.0
36	37.17232431933607	40.0	36.0	41.0	31.0	41.0
37	37.10637818638977	40.0	36.0	41.0	31.0	41.0
38	37.012232373436895	39.0	36.0	41.0	31.0	41.0
39	36.88870929243797	39.0	35.0	41.0	30.0	41.0
40	36.781816772330686	39.0	35.0	41.0	30.0	41.0
41	36.67697098114733	39.0	35.0	41.0	30.0	41.0
42	36.552547816142756	39.0	35.0	41.0	30.0	41.0
43	36.41610067907577	39.0	35.0	41.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	0.0
11	0.0
12	2.0
13	2.0
14	7.0
15	32.0
16	69.0
17	151.0
18	371.0
19	842.0
20	1743.0
21	3529.0
22	6607.0
23	11479.0
24	18897.0
25	29676.0
26	44243.0
27	64960.0
28	91494.0
29	125365.0
30	165964.0
31	215779.0
32	274419.0
33	348227.0
34	447388.0
35	578348.0
36	771010.0
37	1106200.0
38	1776794.0
39	3062214.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.90630040216217	18.211491969057317	12.353401496400593	26.528806132379923
2	19.451031854685855	21.13130893885541	33.633346756597795	25.784312449860934
3	20.576585154321435	22.16414221458497	29.79548127651418	27.463791354579413
4	15.782588163567308	15.513940641471677	32.92408230957707	35.779388885383945
5	15.84460561351954	35.459865623755924	33.10326812936149	15.592260633363047
6	36.054279701542114	33.480916349372116	14.536947125422312	15.927856823663461
7	30.04778252081034	30.03843398066416	20.186209798953904	19.727573699571597
8	27.873804111236478	33.60771754244264	18.984665441989684	19.533812904331196
9	27.218312904495207	14.082214451574727	18.76342759249506	39.93604505143501
10	17.33769321546373	26.789515595825108	32.32574293832599	23.547048250385178
11	36.28042690135913	21.391056213373265	20.69211342939113	21.636403455876476
12	21.927006379859286	24.746952512586905	29.179330476142475	24.146710631411334
13	31.456459912311786	19.572464470900506	23.61164611609706	25.359429500690645
14	22.64040386568149	20.443343855816863	25.098293612607215	31.817958665894437
15	25.7201410087873	27.339854860360692	22.192395580360106	24.747608550491904
16	24.76415163966287	25.64077135624794	24.433355460034008	25.161721544055187
17	24.446705831400664	25.699421144954528	25.294809767048594	24.559063256596215
18	24.625705773778666	24.926728766485823	25.874156840950064	24.573408618785447
19	25.527003449556645	24.671792436604598	26.274996000902274	23.52620811293649
20	24.93866865635674	24.442714934145275	26.26378868669193	24.354827722806053
21	24.63439827601986	24.729644045860113	26.462994596543794	24.172963081576235
22	24.95381219799705	24.417140389815536	25.802954860327894	24.826092551859524
23	24.51762352893067	24.607861542762794	26.0334756461782	24.841039282128335
24	24.35599765706996	24.694633489663524	26.112670355276236	24.836698497990284
25	24.814688426277687	24.387137589627077	25.971797149143548	24.826376834951688
26	24.387476542544658	25.00013940805481	26.128994765145535	24.48338928425499
27	24.845259792650474	25.061457084241717	25.882805607330916	24.21047751577689
28	24.3360541047581	25.41544420381217	25.763198963285166	24.48530272814456
29	24.395841025833352	25.4496456465926	26.034328495454695	24.120184832119353
30	24.888536426450006	25.063414263991625	25.741943335163313	24.30610597439506
31	24.738631765158548	24.738478689647383	26.08802519797857	24.434864347215495
32	24.376455105740735	25.160814024953275	25.842492078068947	24.62023879123704
33	24.253579206135093	24.796494308379145	26.015664217057576	24.934262268428185
34	24.712882277387475	24.99966924755623	26.065096673198983	24.222351801857307
35	24.548829065278284	25.205293394911966	25.823959007252828	24.42191853255692
36	24.484471746798235	25.17104821627121	26.022727558501362	24.3217524784292
37	24.503376572427186	25.188192673521755	26.025264238400673	24.283166515650386
38	24.52068503915398	25.233809175849103	26.004784921799732	24.240720863197183
39	24.102963837113222	25.637447430862625	26.114430723654635	24.145158008369513
40	24.861420193043525	25.086583336003045	25.961213070942957	24.090783400010473
41	24.291957423577323	25.431987292983138	25.900289017499045	24.37576626594049
42	24.444070745815598	25.317169725643858	25.680111762617496	24.55864776592305
43	24.46106212755498	25.053005129232357	25.706714099665064	24.779218643547598
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1797.0
1	1782.0
2	1767.0
3	3279.0
4	4791.0
5	4791.0
6	6471.0
7	8151.0
8	7136.5
9	6122.0
10	8748.0
11	11374.0
12	11374.0
13	23655.5
14	35937.0
15	53505.0
16	71073.0
17	63746.5
18	56420.0
19	56420.0
20	67852.5
21	79285.0
22	66051.0
23	52817.0
24	63226.0
25	73635.0
26	73635.0
27	89983.5
28	106332.0
29	126576.0
30	146820.0
31	170982.0
32	195144.0
33	195144.0
34	223806.5
35	252469.0
36	289121.5
37	325774.0
38	364585.5
39	403397.0
40	403397.0
41	446838.5
42	490280.0
43	534889.5
44	579499.0
45	621154.5
46	662810.0
47	662810.0
48	708708.5
49	754607.0
50	777503.0
51	800399.0
52	798375.5
53	796352.0
54	796352.0
55	761337.5
56	726323.0
57	676375.5
58	626428.0
59	575585.5
60	524743.0
61	524743.0
62	467082.0
63	409421.0
64	353055.5
65	296690.0
66	254266.5
67	211843.0
68	211843.0
69	179628.0
70	147413.0
71	127755.5
72	108098.0
73	88970.0
74	69842.0
75	69842.0
76	58007.5
77	46173.0
78	37189.5
79	28206.0
80	21962.5
81	15719.0
82	15719.0
83	12107.5
84	8496.0
85	6874.5
86	5253.0
87	3879.0
88	2505.0
89	2505.0
90	1768.0
91	1031.0
92	699.5
93	368.0
94	260.5
95	153.0
96	153.0
97	94.5
98	36.0
99	28.0
100	20.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	9145813.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.0999470228678
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.18974675651741	35.414330888948164
2	12.103506412284252	11.401490216190581
3	4.531702259433047	6.403288090281205
4	2.286813551572802	4.308351885210206
5	1.3375106313411367	3.1498339939345006
6	0.8462585656881542	2.391524016693609
7	0.6395409567476326	2.108564162723738
8	0.43926162099491106	1.6551359262431478
9	0.3551990800628612	1.5056872068178926
>10	2.038376198328044	17.969056801226333
>50	0.15705402935176344	5.043467012404836
>100	0.0717118768932843	5.884980578674407
>500	0.0022976143359140803	0.7143711379633964
>1k	9.04486664480706E-4	0.6890591396657242
>5k	2.319195685387992E-5	0.057784782782324705
>10k+	9.276782741551969E-5	1.3030741602399014
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	39269	0.4293658748544279	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	38574	0.4217667691215642	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	26137	0.28578104538109406	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15312	0.1674208733548346	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	2.1867930166514446E-5	1.0933965083257223E-5
2	1.6400947624885836E-4	0.0	0.0	3.2801895249771674E-5	1.0933965083257223E-5
3	2.842830921646878E-4	0.0	0.0	3.2801895249771674E-5	1.0933965083257223E-5
4	2.9521705724794506E-4	0.0	0.0	4.373586033302889E-5	4.373586033302889E-5
5	9.731228924098929E-4	0.0	0.0	6.560379049954335E-5	4.373586033302889E-5
6	0.0013339437401573813	0.0	0.0	7.653775558280057E-5	7.653775558280057E-5
7	0.0015416890767392685	0.0	0.0	7.653775558280057E-5	8.747172066605778E-5
8	0.0019462457848197859	0.0	0.0	9.840568574931502E-5	1.0933965083257223E-4
9	0.0020665194007356154	0.0	0.0	9.840568574931502E-5	1.312075809990867E-4
10	0.0031817838392278522	0.0	0.0	1.312075809990867E-4	1.312075809990867E-4
11	0.0033348593503934533	1.0933965083257223E-5	0.0	1.312075809990867E-4	1.312075809990867E-4
12	0.003564472617141855	1.0933965083257223E-5	0.0	1.312075809990867E-4	1.312075809990867E-4
13	0.0037940858838902566	1.0933965083257223E-5	0.0	1.312075809990867E-4	1.5307551116560113E-4
14	0.004297048277720089	1.0933965083257223E-5	0.0	1.7494344133211557E-4	2.1867930166514447E-4
15	0.0046360011953010625	1.0933965083257223E-5	0.0	2.1867930166514447E-4	3.0615102233120227E-4
16	0.004898416357299236	1.0933965083257223E-5	0.0	2.1867930166514447E-4	3.608208477474884E-4
17	0.0049421522176322655	1.0933965083257223E-5	0.0	2.296132667484017E-4	3.8268877791400286E-4
18	0.005106161693881123	1.0933965083257223E-5	0.0	2.296132667484017E-4	4.592265334968034E-4
19	0.005281105135213239	1.0933965083257223E-5	0.0	2.4054723183165893E-4	4.592265334968034E-4
20	0.006625982840453877	1.0933965083257223E-5	0.0	2.733491270814306E-4	4.592265334968034E-4
21	0.009446945831934242	1.0933965083257223E-5	0.0	4.701604985800606E-4	4.8109446366331786E-4
22	0.00964375720343287	1.0933965083257223E-5	0.0	5.466982541628612E-4	4.920284287465751E-4
23	0.009796832714598473	1.0933965083257223E-5	0.0	5.904341144958901E-4	6.669718700786907E-4
24	0.010212323387762247	1.0933965083257223E-5	0.0	7.653775558280057E-4	6.669718700786907E-4
25	0.010529408375176706	1.0933965083257223E-5	0.0	8.637832415773207E-4	6.888398002452051E-4
26	0.012311644683747633	1.0933965083257223E-5	0.0	0.0010387266829094363	6.997737653284623E-4
27	0.012694333461661636	1.0933965083257223E-5	0.0	0.001957179749903043	7.216416954949768E-4
28	0.013317569471407298	1.0933965083257223E-5	0.0	0.004297048277720089	7.435096256614912E-4
29	0.01335037136665707	1.0933965083257223E-5	0.0	0.00929387032076864	7.544435907447484E-4
30	0.0134706449825729	1.0933965083257223E-5	0.0	0.015187277500644284	7.544435907447484E-4
31	0.013776796004904102	1.0933965083257223E-5	0.0	0.0345841315583426	7.872454859945201E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	17870	0.0	14.628147	1
TATACCG	375	0.0	13.32	5
TAATCCC	2985	0.0	12.519262	5
TTAATCC	3220	0.0	12.180124	4
TCGTTAC	1440	0.0	11.690972	23
TTTAATC	3745	0.0	11.361816	3
TAGATCG	370	0.0	11.0	5
GTATCAA	23980	0.0	10.893244	2
CCTTTAA	4120	0.0	10.731796	1
TAGACTG	1220	0.0	10.463115	5
AACGCAG	27395	0.0	10.4537325	5
ATACCGT	465	0.0	10.344086	6
CTCGTTA	1690	0.0	10.28994	22
TCTATAC	860	0.0	10.110465	3
GTATACG	295	2.8556315E-8	10.033898	1
CGTTACG	1150	0.0	9.973913	24
CTTTAAT	4440	0.0	9.958333	2
GGCGGGT	820	0.0	9.701219	33
TCTACAC	1710	0.0	9.520468	3
CGCGTAA	175	0.0010993957	9.514285	10
>>END_MODULE