FastQCFastQC Report
Thu 9 Feb 2017
SRR2727584.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727584.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9589581
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT990151.032526864312424No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT906900.9457138951117885No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT660830.689112485728No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT376930.3930620117813281No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT139120.14507411741972875No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT111550.11632416473670748No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA313500.020.08741
GCGGGTT11400.014.28070234
GTATCAA450700.013.9683832
GGCGGGT12100.013.30165333
TTAATCC59850.013.198834
CCTTTAA66350.012.8816881
TAATCCC65150.012.6646195
CGGTAAA3550.012.5070439
ACGTCAG28500.012.39824530
TTTAATC68800.012.100293
TTGCACG28150.012.02664326
CGGGAGT36900.011.9322494
GCACGTC28950.011.82210628
GACCGAT29350.011.53492419
CGGGTTT13650.011.52014635
CCGGTAA4350.011.4827598
CCGATTT30150.011.35157521
ACGACCG30300.011.2953817
CGATTTG30500.011.28196722
CACGTCA30500.011.28196729
Produced by FastQC (version 0.11.5)