##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727584.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9589581
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.94017559265624	31.0	31.0	34.0	30.0	34.0
2	32.11968510407285	33.0	31.0	34.0	30.0	34.0
3	32.17368986194496	34.0	31.0	34.0	30.0	34.0
4	35.8254219866332	37.0	35.0	37.0	35.0	37.0
5	35.746295067532145	37.0	35.0	37.0	35.0	37.0
6	35.79785686152503	37.0	35.0	37.0	35.0	37.0
7	35.71566964187486	37.0	35.0	37.0	33.0	37.0
8	35.701904076935165	37.0	35.0	37.0	33.0	37.0
9	37.361239557807586	39.0	37.0	39.0	34.0	39.0
10	37.241149326545134	39.0	37.0	39.0	34.0	39.0
11	37.313820697692634	39.0	37.0	39.0	34.0	39.0
12	37.171583826238084	39.0	37.0	39.0	33.0	39.0
13	37.20391641720321	39.0	37.0	39.0	33.0	39.0
14	38.32483306622051	40.0	38.0	41.0	33.0	41.0
15	38.32712555428647	40.0	38.0	41.0	33.0	41.0
16	38.24532802840917	40.0	38.0	41.0	33.0	41.0
17	38.256538007239314	40.0	38.0	41.0	33.0	41.0
18	38.19999643362937	40.0	38.0	41.0	33.0	41.0
19	38.21872530197096	40.0	38.0	41.0	33.0	41.0
20	38.136690748010786	40.0	37.0	41.0	33.0	41.0
21	38.038424202267024	40.0	37.0	41.0	33.0	41.0
22	37.9491858924806	40.0	37.0	41.0	33.0	41.0
23	37.84390569306417	40.0	37.0	41.0	32.0	41.0
24	37.727205599493864	40.0	37.0	41.0	32.0	41.0
25	37.9494708892912	40.0	37.0	41.0	33.0	41.0
26	37.864520983763526	40.0	37.0	41.0	33.0	41.0
27	37.80107973434919	40.0	37.0	41.0	32.0	41.0
28	37.721184898485134	40.0	37.0	41.0	32.0	41.0
29	37.638839799152855	40.0	37.0	41.0	32.0	41.0
30	37.51445229984501	40.0	37.0	41.0	32.0	41.0
31	37.3721143812227	40.0	36.0	41.0	32.0	41.0
32	37.230629993114405	40.0	36.0	41.0	31.0	41.0
33	37.113302343449625	39.0	35.0	41.0	31.0	41.0
34	36.99712542185107	39.0	35.0	41.0	31.0	41.0
35	36.82593952749343	39.0	35.0	41.0	30.0	41.0
36	36.71606955507232	39.0	35.0	41.0	30.0	41.0
37	36.6203682934635	39.0	35.0	41.0	30.0	41.0
38	36.46729393077758	39.0	35.0	41.0	30.0	41.0
39	36.310804924636436	39.0	35.0	41.0	30.0	41.0
40	36.152549313677	39.0	35.0	41.0	29.0	41.0
41	36.01994185147401	39.0	35.0	41.0	28.0	41.0
42	35.81657040072971	39.0	35.0	41.0	27.0	41.0
43	35.6413236407305	38.0	35.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	2.0
13	9.0
14	22.0
15	67.0
16	179.0
17	359.0
18	821.0
19	1615.0
20	3213.0
21	5674.0
22	9809.0
23	16226.0
24	25170.0
25	38550.0
26	55865.0
27	79054.0
28	110456.0
29	146873.0
30	191294.0
31	243145.0
32	304693.0
33	384103.0
34	494840.0
35	628899.0
36	835675.0
37	1224243.0
38	1784581.0
39	3004143.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.67083202071081	19.519476398395298	13.199502668573318	24.61018891232057
2	19.614569187120896	22.853991222348505	33.577567153351126	23.953872437179477
3	20.24997755376382	24.02610708434498	31.074246101054886	24.64966926083632
4	14.846289947391861	16.76232778053598	34.22836722480367	34.163015047268495
5	14.933989295257009	36.72096830925147	33.23974217434526	15.105300221146262
6	33.19387989944504	35.308727253046825	14.889878921717226	16.60751392579092
7	28.218824159262017	31.734660774021307	21.1582341293118	18.88828093740488
8	27.457059906996978	33.89373320899005	19.431203511394294	19.21800337261868
9	27.255841522168694	14.039362095173919	20.138950805045603	38.56584557761178
10	17.41877981947282	27.87591032392343	31.953638016092672	22.75167184051107
11	34.53617003704333	22.642668120744798	21.194982345944	21.626179496267877
12	21.9503229598874	26.854374555050946	29.083272772814578	22.11202971224707
13	31.533536241051618	20.50612013183892	24.578195856523866	23.382147770585597
14	21.9950485845002	22.121331474232296	27.28770944215394	28.595910499113568
15	25.50501424410514	28.071476741267425	23.905799429610116	22.51770958501732
16	23.00016027811851	26.024557277320042	26.767207034384505	24.208075410176942
17	23.36301241941645	26.144677228337716	26.702762091482413	23.789548260763425
18	23.39894725327415	24.287390658674244	28.127714860534574	24.185947227517033
19	24.74557543233641	24.42334028984165	29.168928235759207	21.662156042062737
20	23.726114832337306	24.522426996549694	29.405174219812107	22.346283951300897
21	23.437937486528348	24.97984009937452	29.541321982680994	22.040900431416137
22	23.355817110257476	25.043367379659237	28.562155113972132	23.038660396111155
23	23.082259798420807	24.72510529917835	28.860708304148012	23.331926598252835
24	22.457800815280667	25.3390633021401	29.134620167450485	23.068515715128743
25	22.851916053475122	24.858593926053704	29.10019739131459	23.189292629156583
26	22.674160633295656	25.472614496921192	28.999463063088992	22.853761806694163
27	23.382982009328664	25.68660716250272	28.749191440168243	22.181219388000372
28	22.505540127352802	25.566998182715178	28.615681957324306	23.31177973260771
29	22.588098478963783	25.861588738861478	29.001924067381047	22.548388714793692
30	22.92281591865171	25.603381419897282	28.692098226189444	22.781704435261563
31	23.015781398582483	25.104496223557632	28.97497815597991	22.904744221879973
32	22.291015634572563	26.068521659079785	28.50758547219112	23.132877234156528
33	22.313654788462603	25.30261749705227	29.107152856834933	23.276574857650193
34	23.045845277285835	25.53269011440646	29.193246295119675	22.22821831318803
35	23.0220381891555	26.200884063651998	28.471786202129163	22.305291545063337
36	22.724611221282764	25.939736053118484	28.608695207851103	22.72695751774765
37	22.880582582283836	26.25871766451527	28.14036400547636	22.720335747724533
38	22.732265361750425	25.951613527222932	28.547608075889862	22.76851303513678
39	22.460887498630022	26.571922172616304	28.36917483673166	22.598015492022018
40	23.173723648614054	25.981072582837562	28.416809868960907	22.42839389958748
41	22.69596554844263	26.177796506437563	28.35347029239338	22.772767652726433
42	22.64111435108583	26.32770920856709	27.692794919819754	23.338381520527328
43	22.40263677839522	26.033191648310805	28.07921430560939	23.484957267684585
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	4527.0
1	4435.5
2	4344.0
3	7812.5
4	11281.0
5	11281.0
6	14823.0
7	18365.0
8	15896.0
9	13427.0
10	19873.0
11	26319.0
12	26319.0
13	56268.5
14	86218.0
15	125247.5
16	164277.0
17	148198.5
18	132120.0
19	132120.0
20	157262.5
21	182405.0
22	143349.0
23	104293.0
24	118388.5
25	132484.0
26	132484.0
27	157791.0
28	183098.0
29	211533.0
30	239968.0
31	272662.5
32	305357.0
33	305357.0
34	334325.5
35	363294.0
36	389041.5
37	414789.0
38	444002.5
39	473216.0
40	473216.0
41	503112.0
42	533008.0
43	555766.5
44	578525.0
45	598417.0
46	618309.0
47	618309.0
48	653141.5
49	687974.0
50	701221.5
51	714469.0
52	699926.0
53	685383.0
54	685383.0
55	653235.0
56	621087.0
57	587532.0
58	553977.0
59	509173.5
60	464370.0
61	464370.0
62	410474.0
63	356578.0
64	308746.5
65	260915.0
66	223947.0
67	186979.0
68	186979.0
69	162293.0
70	137607.0
71	120305.5
72	103004.0
73	87185.5
74	71367.0
75	71367.0
76	62897.0
77	54427.0
78	47033.0
79	39639.0
80	32015.5
81	24392.0
82	24392.0
83	20135.5
84	15879.0
85	13365.0
86	10851.0
87	8892.5
88	6934.0
89	6934.0
90	4803.5
91	2673.0
92	1785.0
93	897.0
94	649.5
95	402.0
96	402.0
97	258.5
98	115.0
99	76.5
100	38.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	9589581.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.000292534014804
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.49462439072938	29.833127285180293
2	12.114789966933914	9.449607053971404
3	4.014273367577091	4.696735069410337
4	1.976730608428056	3.0837228795854097
5	1.1278313206629085	2.199287571743884
6	0.7666952599895662	1.7940803654421367
7	0.5120625703874265	1.3979413028580427
8	0.3801902393094633	1.1862024441316927
9	0.2974852339522958	1.0441810033820431
>10	1.9347403760308053	14.606033635646146
>50	0.1982521565600532	5.400292493109148
>100	0.15844240572329651	12.242039461815613
>500	0.01620684040519338	4.336103603841688
>1k	0.007275510048655121	4.819198719844025
>5k	2.3985195711987754E-4	0.6005666221460418
>10k+	1.59901304746585E-4	3.310880487892031
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	99015	1.032526864312424	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	90690	0.9457138951117885	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	66083	0.689112485728	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	37693	0.3930620117813281	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13912	0.14507411741972875	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11155	0.11632416473670748	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.1283952865093895E-5	0.0	0.0	2.0855968576729264E-5	0.0
2	6.361070415902425E-4	0.0	0.0	3.1283952865093895E-5	0.0
3	8.968066487993584E-4	0.0	0.0	3.1283952865093895E-5	0.0
4	9.698025388179108E-4	0.0	0.0	6.256790573018779E-5	0.0
5	0.003149251255086119	0.0	0.0	1.4599178003710486E-4	0.0
6	0.004327613479671323	0.0	0.0	1.7727573290219874E-4	1.0427984288364632E-5
7	0.004817728741224461	0.0	0.0	1.877037171905634E-4	1.0427984288364632E-5
8	0.006194222667288592	0.0	0.0	2.294156543440219E-4	1.0427984288364632E-5
9	0.0065383461488046244	0.0	0.0	2.606996072091158E-4	3.1283952865093895E-5
10	0.009739737325332566	0.0	0.0	3.545514658043975E-4	3.1283952865093895E-5
11	0.010459268241229726	0.0	0.0	3.9626340295785604E-4	3.1283952865093895E-5
12	0.011064091329954876	0.0	0.0	4.0669138724622064E-4	3.1283952865093895E-5
13	0.011846190151582222	0.0	0.0	4.484033243996792E-4	3.1283952865093895E-5
14	0.013587663527739116	0.0	0.0	5.213992144182316E-4	6.256790573018779E-5
15	0.01447404219225011	0.0	0.0	5.526831672833255E-4	7.299589001855243E-5
16	0.015370848841049467	0.0	0.0	5.839671201484194E-4	9.38518585952817E-5
17	0.015673260385412044	0.0	0.0	6.25679057301878E-4	9.38518585952817E-5
18	0.015944387976909523	0.0	0.0	6.361070415902425E-4	1.0427984288364632E-4
19	0.016778626719978693	0.0	0.0	7.09102931608795E-4	1.0427984288364632E-4
20	0.020918536482459452	0.0	0.0	8.863786645109937E-4	1.0427984288364632E-4
21	0.030480998074889822	0.0	0.0	0.0014599178003710485	1.1470782717201095E-4
22	0.031148389069345157	0.0	0.0	0.0017101894232917998	1.2513581146037558E-4
23	0.03186791998524232	0.0	0.0	0.001897893140482363	1.4599178003710486E-4
24	0.03334869375419009	0.0	0.0	0.0020855968576729265	1.4599178003710486E-4
25	0.03424550040298945	0.0	0.0	0.002325440496305313	1.564197643254695E-4
26	0.03909451309707901	0.0	0.0	0.0028989796321653677	1.6684774861383411E-4
27	0.040168595478780565	0.0	0.0	0.004025201935308748	1.6684774861383411E-4
28	0.042129056524993116	0.0	0.0	0.00765414046765964	1.6684774861383411E-4
29	0.04247318000650915	0.0	0.0	0.015297852951030916	1.7727573290219874E-4
30	0.04301543518950411	0.0	0.0	0.02510015818209367	1.7727573290219874E-4
31	0.04435021717841478	0.0	0.0	0.049897904819824764	1.7727573290219874E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	31350	0.0	20.0874	1
GCGGGTT	1140	0.0	14.280702	34
GTATCAA	45070	0.0	13.968383	2
GGCGGGT	1210	0.0	13.301653	33
TTAATCC	5985	0.0	13.19883	4
CCTTTAA	6635	0.0	12.881688	1
TAATCCC	6515	0.0	12.664619	5
CGGTAAA	355	0.0	12.507043	9
ACGTCAG	2850	0.0	12.398245	30
TTTAATC	6880	0.0	12.10029	3
TTGCACG	2815	0.0	12.026643	26
CGGGAGT	3690	0.0	11.932249	4
GCACGTC	2895	0.0	11.822106	28
GACCGAT	2935	0.0	11.534924	19
CGGGTTT	1365	0.0	11.520146	35
CCGGTAA	435	0.0	11.482759	8
CCGATTT	3015	0.0	11.351575	21
ACGACCG	3030	0.0	11.29538	17
CGATTTG	3050	0.0	11.281967	22
CACGTCA	3050	0.0	11.281967	29
>>END_MODULE