FastQCFastQC Report
Thu 9 Feb 2017
SRR2727583.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727583.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10265486
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT678470.6609234087894134No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT644760.6280852168129205No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT462040.4500907214719303No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT262010.255233897352741No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA265900.018.8548341
ACGGACT11600.017.22413827
TATCTCG13350.015.10486936
CGGACTC13700.014.44890628
CACGAGA14900.013.6577221
ACGAGAC15350.013.61889322
GTATCAA380750.013.1479982
CGAGACG15900.013.14779923
TAATCCC53300.012.9812375
TTAATCC56850.012.8540014
GGACCGT11100.012.56
TATACAC23050.012.4403483
CCCACGA16550.012.1842919
GGCGGGT10350.012.1545933
CCACGAG16650.012.11111120
TCTCCGA17350.012.0489911
GCCCACG16900.011.931951518
TTTAATC64200.011.5552963
TCTATAC9950.011.5276383
CCTTTAA70450.011.5017741
Produced by FastQC (version 0.11.5)