##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727583.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10265486
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.950127543888325	31.0	31.0	34.0	30.0	34.0
2	32.12980973331413	33.0	31.0	34.0	30.0	34.0
3	32.19524024483595	34.0	31.0	34.0	30.0	34.0
4	35.84539972096791	37.0	35.0	37.0	35.0	37.0
5	35.75522571459354	37.0	35.0	37.0	35.0	37.0
6	35.79985000223078	37.0	35.0	37.0	35.0	37.0
7	35.71676801273705	37.0	35.0	37.0	33.0	37.0
8	35.69674655442519	37.0	35.0	37.0	33.0	37.0
9	37.360715021188476	39.0	37.0	39.0	34.0	39.0
10	37.23585020718941	39.0	37.0	39.0	33.0	39.0
11	37.30791752090452	39.0	37.0	39.0	34.0	39.0
12	37.15652098692648	39.0	37.0	39.0	33.0	39.0
13	37.19147539629395	39.0	37.0	39.0	33.0	39.0
14	38.337490597132955	40.0	38.0	41.0	33.0	41.0
15	38.33982044298731	40.0	38.0	41.0	33.0	41.0
16	38.26726187147886	40.0	38.0	41.0	33.0	41.0
17	38.244635373327675	40.0	38.0	41.0	33.0	41.0
18	38.181023480037865	40.0	38.0	41.0	33.0	41.0
19	38.18671342009526	40.0	38.0	41.0	33.0	41.0
20	38.10713102136616	40.0	37.0	41.0	33.0	41.0
21	38.013593998374745	40.0	37.0	41.0	33.0	41.0
22	37.913259245592464	40.0	37.0	41.0	32.0	41.0
23	37.81520465762654	40.0	37.0	41.0	32.0	41.0
24	37.70630099734197	40.0	37.0	41.0	32.0	41.0
25	37.938145549075806	40.0	37.0	41.0	33.0	41.0
26	37.875038551511345	40.0	37.0	41.0	33.0	41.0
27	37.81704860344654	40.0	37.0	41.0	32.0	41.0
28	37.74831050375988	40.0	37.0	41.0	32.0	41.0
29	37.67807242638098	40.0	37.0	41.0	32.0	41.0
30	37.56486707010267	40.0	37.0	41.0	32.0	41.0
31	37.44682102727528	40.0	36.0	41.0	32.0	41.0
32	37.32662739981332	40.0	36.0	41.0	31.0	41.0
33	37.23579721408222	40.0	36.0	41.0	31.0	41.0
34	37.12847886597868	40.0	36.0	41.0	31.0	41.0
35	36.985252427405776	39.0	35.0	41.0	31.0	41.0
36	36.88162577008044	39.0	35.0	41.0	30.0	41.0
37	36.798682984906904	39.0	35.0	41.0	30.0	41.0
38	36.671586907819076	39.0	35.0	41.0	30.0	41.0
39	36.53153089878063	39.0	35.0	41.0	30.0	41.0
40	36.400208329152655	39.0	35.0	41.0	30.0	41.0
41	36.2771399230392	39.0	35.0	41.0	29.0	41.0
42	36.11037061469861	39.0	35.0	41.0	28.0	41.0
43	35.952696832863055	39.0	35.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	3.0
12	3.0
13	11.0
14	21.0
15	49.0
16	119.0
17	292.0
18	638.0
19	1343.0
20	2736.0
21	5325.0
22	9367.0
23	15911.0
24	24995.0
25	38321.0
26	55882.0
27	80660.0
28	112218.0
29	151164.0
30	198376.0
31	255710.0
32	323256.0
33	407168.0
34	523763.0
35	671616.0
36	889723.0
37	1284375.0
38	1940664.0
39	3271777.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.76608043691258	19.058181950664586	12.935062207478534	25.240675404944295
2	19.44588887462318	22.325392095415648	33.98050516069088	24.24821386927029
3	20.261583328836064	23.650015206294178	30.57481155787461	25.51358990699515
4	15.077737186529697	16.62630488220431	34.29857095903691	33.99738697222908
5	15.075662272589918	36.431153868409154	33.501336419922055	14.991847439078871
6	33.76623376623377	35.17969826270281	14.964639764741777	16.08942820632165
7	28.63307202406199	31.09240030135933	20.996492518717574	19.278035155861104
8	27.256498133649004	34.12904172291501	19.403513871627702	19.21094627180827
9	27.139932780581454	14.232623764719957	19.549468968152116	39.07797448654647
10	17.15254397112811	27.56498815545606	32.280556419832436	23.001911453583396
11	35.24198464641616	22.538406851852898	20.915950788886175	21.30365771284477
12	21.667907393765866	26.282038668213076	29.326463452387934	22.72359048563312
13	31.557268696289686	20.46805187791401	23.874894963570163	24.099784462226143
14	22.08248104376159	21.70254774104217	26.270261339794338	29.944709875401905
15	25.61354620716447	28.19269345844902	22.94822670840913	23.245533625977377
16	23.693101330029577	26.283012806212973	25.564342496789727	24.459543366967722
17	23.689487278049963	26.105982707491883	25.998730113703335	24.20579990075482
18	23.911074448886296	24.954181419174894	26.850604053232356	24.284140078706454
19	25.00945400928899	24.881608138182646	27.581139363494334	22.52779848903403
20	24.411781380832824	24.92593141717791	27.6507707477269	23.01151645426237
21	24.170565329298583	25.22078350698642	27.554165482277217	23.054485681437782
22	23.98101755727883	25.317340065536108	27.078396483128024	23.62324589405704
23	23.520669162667993	25.33164041137458	27.2200751138329	23.92761531212453
24	23.294698370832126	25.43237602194382	27.39212736737452	23.88079823984953
25	23.65573339635357	25.214938678987043	27.320927620962127	23.808400303697262
26	23.427561052637934	25.59323542986664	27.400992023173576	23.578211494321845
27	23.977111263899246	25.644874485241125	27.163029592558992	23.21498465830064
28	23.20742534742145	26.016323045981455	26.98989604583748	23.786355560759617
29	23.340531563727232	26.231266595658504	27.215009596233436	23.21319224438083
30	23.691766760969717	25.958459248787634	27.079468034927913	23.270305955314733
31	23.459337434194545	25.536189908592732	27.43996728454941	23.564505372663312
32	23.030015334880392	26.040958995998825	27.102262864125475	23.8267628049953
33	23.075234820835565	25.635610432862116	27.43472642210997	23.854428324192348
34	23.483759073851935	25.869637345957123	27.48321901174479	23.163384568446148
35	23.596057702479943	26.2059487490412	26.952011819021525	23.24598172945733
36	23.180198190324354	26.126439605489697	27.33388365636074	23.35947854782521
37	23.44923562313562	26.19781469674207	26.93574371442326	23.417205965699043
38	23.205214054161683	26.110346845731414	27.391689005274568	23.292750094832336
39	23.045270335958765	26.387810572241783	27.416899696711877	23.15001939508758
40	23.619641583457422	25.890844330214858	27.422637369531262	23.066876716796457
41	22.987562400844926	26.02772046058024	27.456888061607604	23.527829076967226
42	22.929766793311103	26.006503734942505	27.222831924372603	23.840897547373793
43	22.860544547038494	25.899358296333947	27.195906750055478	24.04419040657208
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2564.0
1	2689.5
2	2815.0
3	5422.0
4	8029.0
5	8029.0
6	10644.5
7	13260.0
8	11910.5
9	10561.0
10	15578.5
11	20596.0
12	20596.0
13	42047.0
14	63498.0
15	93229.0
16	122960.0
17	112634.0
18	102308.0
19	102308.0
20	121742.0
21	141176.0
22	117629.0
23	94082.0
24	109061.0
25	124040.0
26	124040.0
27	147701.5
28	171363.0
29	196740.5
30	222118.0
31	252221.0
32	282324.0
33	282324.0
34	316394.0
35	350464.0
36	388020.5
37	425577.0
38	461346.5
39	497116.0
40	497116.0
41	538121.5
42	579127.0
43	625241.5
44	671356.0
45	701011.0
46	730666.0
47	730666.0
48	774104.5
49	817543.0
50	829698.5
51	841854.0
52	831283.0
53	820712.0
54	820712.0
55	770952.5
56	721193.0
57	668310.5
58	615428.0
59	551903.0
60	488378.0
61	488378.0
62	425977.5
63	363577.0
64	317598.0
65	271619.0
66	237757.0
67	203895.0
68	203895.0
69	177174.5
70	150454.0
71	135816.0
72	121178.0
73	100152.0
74	79126.0
75	79126.0
76	66866.5
77	54607.0
78	44689.5
79	34772.0
80	27023.0
81	19274.0
82	19274.0
83	15328.5
84	11383.0
85	9631.0
86	7879.0
87	5853.0
88	3827.0
89	3827.0
90	2778.0
91	1729.0
92	1196.5
93	664.0
94	480.5
95	297.0
96	297.0
97	181.5
98	66.0
99	48.5
100	31.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1.0265486E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.60195932494628
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.2324708290365	43.78191045063756
2	9.724141789400619	10.230178217521233
3	2.7256969508772415	4.301310004265243
4	1.2305912046326644	2.5892603396689626
5	0.6945135960282542	1.8266387964450201
6	0.4196129417351251	1.3243477736023261
7	0.2919371636957728	1.0749526767115656
8	0.2177483544373882	0.9163192066551584
9	0.1725523481640496	0.8168932439594375
>10	1.059611385261418	10.784452270724245
>50	0.12146300187041374	4.491633155976118
>100	0.09858242954131574	10.168856835582044
>500	0.00795445231665121	2.80482645839605
>1k	0.002938727582687363	2.5184254958888546
>5k	1.1089525199605896E-4	0.3795927656496008
>10k+	7.393016799737265E-5	1.9904023083165527
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	67847	0.6609234087894134	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	64476	0.6280852168129205	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	46204	0.4500907214719303	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	26201	0.255233897352741	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.896551999583848E-5	0.0	0.0	4.8706899994798106E-5	2.9224139996878863E-5
2	3.019827799677482E-4	0.0	0.0	5.8448279993757726E-5	2.9224139996878863E-5
3	4.286207199542233E-4	0.0	0.0	5.8448279993757726E-5	2.9224139996878863E-5
4	4.675862399500618E-4	0.0	0.0	1.0715517998855582E-4	3.896551999583848E-5
5	0.0018118966798064894	0.0	0.0	1.7534483998127317E-4	3.896551999583848E-5
6	0.0025814656997242996	0.0	0.0	1.9482759997919242E-4	5.8448279993757726E-5
7	0.0029029312396899667	0.0	0.0	2.0456897997815204E-4	5.8448279993757726E-5
8	0.003545862319621302	0.0	0.0	2.2405173997607126E-4	5.8448279993757726E-5
9	0.0037406899196004944	0.0	0.0	2.6301725997190977E-4	6.818965999271735E-5
10	0.005455172799417388	0.0	0.0	3.6043105996150595E-4	6.818965999271735E-5
11	0.005844827999375773	0.0	0.0	3.6043105996150595E-4	7.793103999167696E-5
12	0.006117586639346642	0.0	0.0	3.7017243996046557E-4	8.767241999063659E-5
13	0.006692328059285259	0.0	0.0	3.8965519995838485E-4	9.741379998959621E-5
14	0.007510603979197867	0.0	0.0	4.8706899994798103E-4	1.1689655998751545E-4
15	0.008007414359144809	0.0	0.0	5.552586599406983E-4	1.1689655998751545E-4
16	0.008572414399084466	0.0	0.0	5.844827999375772E-4	1.363793199854347E-4
17	0.008786724759061578	0.0	0.0	5.942241799365369E-4	1.363793199854347E-4
18	0.008923104079047013	0.0	0.0	6.331896999323753E-4	1.7534483998127317E-4
19	0.009400431698996034	0.0	0.0	6.721552199282138E-4	1.7534483998127317E-4
20	0.011416897358780676	0.0	0.0	8.767241999063658E-4	1.9482759997919242E-4
21	0.016005087338290657	0.0	0.0	0.0013150862998595487	2.0456897997815204E-4
22	0.016307070118258406	0.0	0.0	0.0015488794198345796	2.0456897997815204E-4
23	0.01668698393821783	9.74137999895962E-6	0.0	0.0017047414998179336	2.4353449997399052E-4
24	0.017222759838160608	9.74137999895962E-6	0.0	0.0018995690997971261	2.4353449997399052E-4
25	0.01770008745810963	9.74137999895962E-6	0.0	0.002182069119766955	2.5327587997295016E-4
26	0.020047760037858898	9.74137999895962E-6	0.0	0.0029224139996878863	2.6301725997190977E-4
27	0.020632242837796477	9.74137999895962E-6	0.0	0.005669483159394499	2.82500019969829E-4
28	0.021606380837692438	9.74137999895962E-6	0.0	0.013725604418534106	2.82500019969829E-4
29	0.021684311877684114	9.74137999895962E-6	0.0	0.029087760676893427	2.82500019969829E-4
30	0.021888880857662267	9.74137999895962E-6	0.0	0.04845362411482515	2.82500019969829E-4
31	0.02237594985761025	9.74137999895962E-6	0.0	0.10177793822913012	2.922413999687886E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	26590	0.0	18.854834	1
ACGGACT	1160	0.0	17.224138	27
TATCTCG	1335	0.0	15.104869	36
CGGACTC	1370	0.0	14.448906	28
CACGAGA	1490	0.0	13.65772	21
ACGAGAC	1535	0.0	13.618893	22
GTATCAA	38075	0.0	13.147998	2
CGAGACG	1590	0.0	13.147799	23
TAATCCC	5330	0.0	12.981237	5
TTAATCC	5685	0.0	12.854001	4
GGACCGT	1110	0.0	12.5	6
TATACAC	2305	0.0	12.440348	3
CCCACGA	1655	0.0	12.18429	19
GGCGGGT	1035	0.0	12.15459	33
CCACGAG	1665	0.0	12.111111	20
TCTCCGA	1735	0.0	12.04899	11
GCCCACG	1690	0.0	11.9319515	18
TTTAATC	6420	0.0	11.555296	3
TCTATAC	995	0.0	11.527638	3
CCTTTAA	7045	0.0	11.501774	1
>>END_MODULE