FastQCFastQC Report
Thu 9 Feb 2017
SRR2727582.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727582.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9064156
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT300090.3310732957376285No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT294650.3250716338068321No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT190900.2106097909171025No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114060.125836316144603No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA197250.014.3029161
TAATCCC47900.014.0970775
TTAATCC52050.013.4351594
TTTAATC56250.012.3662223
CCTTTAA57600.012.3654511
TATACTG10050.012.1492545
TAGTACT6550.011.58015254
TCGTTAC23850.011.40251623
CTTTAAT61900.011.2673672
GGCGGGT10750.011.01395433
ATGGTTG95100.010.97160832
GGGTAAG68400.010.87280751
TTAGTAC3751.8189894E-1210.85333253
TTTATAC10600.010.8207543
GCACTCG31650.010.81358613
TACGGAT46750.010.76363727
ACGGATG46750.010.76363728
GTATCAA261750.010.7360082
CTCGTTA26150.010.68260122
GGCGAAT10050.010.67661837
Produced by FastQC (version 0.11.5)