##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727582.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9064156
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.95773395780037	31.0	31.0	34.0	30.0	34.0
2	32.148692939530164	33.0	31.0	34.0	30.0	34.0
3	32.22160353374324	34.0	31.0	34.0	30.0	34.0
4	35.86546976905517	37.0	35.0	37.0	35.0	37.0
5	35.757453424234974	37.0	35.0	37.0	35.0	37.0
6	35.792261629212916	37.0	35.0	37.0	35.0	37.0
7	35.70823902412977	37.0	35.0	37.0	33.0	37.0
8	35.685141341344966	37.0	35.0	37.0	33.0	37.0
9	37.3574930749206	39.0	37.0	39.0	34.0	39.0
10	37.22807672330441	39.0	37.0	39.0	33.0	39.0
11	37.29604278655398	39.0	37.0	39.0	34.0	39.0
12	37.137858064225725	39.0	37.0	39.0	33.0	39.0
13	37.173103375537664	39.0	37.0	39.0	33.0	39.0
14	38.33838285660573	40.0	38.0	41.0	33.0	41.0
15	38.33757892075114	40.0	38.0	41.0	33.0	41.0
16	38.2513573243885	40.0	38.0	41.0	33.0	41.0
17	38.20576300760931	40.0	38.0	41.0	33.0	41.0
18	38.12346455643526	40.0	37.0	41.0	33.0	41.0
19	38.1130363378565	40.0	37.0	41.0	33.0	41.0
20	38.02251737503194	40.0	37.0	41.0	33.0	41.0
21	37.920632875250604	40.0	37.0	41.0	32.0	41.0
22	37.83536536661549	40.0	37.0	41.0	32.0	41.0
23	37.726226909598644	40.0	37.0	41.0	32.0	41.0
24	37.61323039894724	40.0	37.0	41.0	32.0	41.0
25	37.86094215501145	40.0	37.0	41.0	32.0	41.0
26	37.81464407717608	40.0	37.0	41.0	32.0	41.0
27	37.768230930712136	40.0	37.0	41.0	32.0	41.0
28	37.69881685619709	40.0	37.0	41.0	32.0	41.0
29	37.63965194332489	40.0	37.0	41.0	32.0	41.0
30	37.544513576332974	40.0	37.0	41.0	32.0	41.0
31	37.444895586527856	40.0	37.0	41.0	31.0	41.0
32	37.336112816240146	40.0	36.0	41.0	31.0	41.0
33	37.254914081355174	40.0	36.0	41.0	31.0	41.0
34	37.16085248312143	40.0	36.0	41.0	31.0	41.0
35	37.025384161525906	39.0	36.0	41.0	30.0	41.0
36	36.93444331717151	39.0	36.0	41.0	30.0	41.0
37	36.866623213457494	39.0	35.0	41.0	30.0	41.0
38	36.74538092680665	39.0	35.0	41.0	30.0	41.0
39	36.612519356462975	39.0	35.0	41.0	30.0	41.0
40	36.50365284975236	39.0	35.0	41.0	30.0	41.0
41	36.3971395682069	39.0	35.0	41.0	30.0	41.0
42	36.25659531896847	39.0	35.0	41.0	29.0	41.0
43	36.11979581993072	39.0	35.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	5.0
14	16.0
15	37.0
16	106.0
17	259.0
18	498.0
19	1124.0
20	2382.0
21	4694.0
22	8467.0
23	14159.0
24	22730.0
25	34775.0
26	50453.0
27	71795.0
28	99639.0
29	133895.0
30	175325.0
31	224851.0
32	284716.0
33	358114.0
34	459925.0
35	589607.0
36	780749.0
37	1099806.0
38	1773657.0
39	2872370.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.53972945743652	17.438876824273546	12.091594628336052	26.92979908995388
2	20.42449401797586	20.925588659330224	32.48776830407596	26.162149018617946
3	21.120057951341526	21.766461212715228	29.42479145328037	27.688689382662872
4	16.292217389021108	15.165813562785107	32.24972076826568	36.2922482799281
5	16.40981245247765	35.084457946222464	32.45819026062659	16.047539340673307
6	36.83918282077228	32.49182825185268	14.24633468356017	16.42265424381487
7	30.520326437453193	29.722822510998263	19.576130419644144	20.180720631904396
8	28.53344536435604	32.55547455273277	18.832784872634583	20.07829521027661
9	27.89438972586085	13.6278325306846	18.104807551855902	40.37297019159865
10	17.684470567364464	26.392970288684353	31.57099237921324	24.351566764737942
11	37.0497815792226	20.902817647886906	19.87144749053304	22.17595328235745
12	22.720394485708322	24.452447641015887	27.945624501608314	24.881533371667476
13	32.32272260097906	19.108375892912697	22.671884729256643	25.89701677685159
14	23.273452045617926	20.06981124331929	24.227859714682758	32.428876996380026
15	26.792996501825435	26.70976757240277	21.41568393130039	25.08155199447141
16	25.45838796243136	24.8453799780145	23.854653428294924	25.841578631259214
17	25.420292854624304	24.708014734080038	24.32434966918045	25.54734274211521
18	25.45799079362712	24.191860775564763	24.90846362308857	25.44168480771955
19	26.694851677310055	23.913666093125492	25.190376246834234	24.201105982730216
20	25.944952845030468	23.681785706247773	25.25215806082773	25.121103387894028
21	25.54000615170348	24.158244849272233	25.56029485812027	24.74145414090402
22	25.84246122860198	23.918785157713526	24.551728809610076	25.687024804074422
23	25.399860726139313	23.928228949281102	24.985304754242975	25.686605570336607
24	25.01306244067291	23.99182008782726	25.163170183743528	25.8319472877563
25	25.509821322580944	23.668381259104542	24.971878242166177	25.849919176148333
26	25.188655182015847	24.226006260262952	25.161581508526552	25.423757049194652
27	25.95477174046872	24.324327604246882	24.826569622146838	24.894331033137558
28	25.0173540702521	24.93970756902242	24.4543672902364	25.58857107048908
29	25.223539841988597	24.89026005289406	24.952108061688257	24.934092043429086
30	25.8984399650668	24.33098018171797	24.618431103789476	25.152148749425763
31	25.582006752752267	24.047545077556034	25.00890320069513	25.36154496899656
32	25.130381692459842	24.473696172042935	24.76974138573961	25.626180749757616
33	25.027393615026046	23.922536196420275	25.126498264151675	25.923571924402005
34	25.67466844127572	24.318303877382515	25.09924807119383	24.90777961014793
35	25.567410799196306	24.51918303259564	24.749220997520343	25.16418517068771
36	25.315010024099323	24.422439331361907	25.026841991686815	25.235708652851958
37	25.402089284429792	24.631096375658142	24.90860704515677	25.0582072947553
38	25.52607214615459	24.311960208981397	25.029236036979064	25.13273160788495
39	24.899019831520995	24.999977935066433	25.132919159820283	24.96808307359229
40	26.005521087677664	24.1386291233293	25.044990399547405	24.810859389445636
41	25.153516775307043	24.777662696890918	24.823017167842213	25.245803359959822
42	25.335011886379714	24.512596649925268	24.658776834820586	25.49361462887444
43	25.217813991727418	24.276711477604753	24.758344847551168	25.747129683116665
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	417.0
1	652.0
2	887.0
3	1948.0
4	3009.0
5	3009.0
6	4342.5
7	5676.0
8	4960.5
9	4245.0
10	5878.0
11	7511.0
12	7511.0
13	16558.5
14	25606.0
15	38989.5
16	52373.0
17	46479.0
18	40585.0
19	40585.0
20	50392.0
21	60199.0
22	50436.0
23	40673.0
24	49761.5
25	58850.0
26	58850.0
27	73311.5
28	87773.0
29	105569.0
30	123365.0
31	143915.0
32	164465.0
33	164465.0
34	188213.0
35	211961.0
36	242861.0
37	273761.0
38	308685.5
39	343610.0
40	343610.0
41	384078.5
42	424547.0
43	470705.0
44	516863.0
45	563970.0
46	611077.0
47	611077.0
48	675258.5
49	739440.0
50	779676.0
51	819912.0
52	827328.5
53	834745.0
54	834745.0
55	801106.0
56	767467.0
57	720769.0
58	674071.0
59	621766.0
60	569461.0
61	569461.0
62	510135.5
63	450810.0
64	393121.0
65	335432.0
66	292283.5
67	249135.0
68	249135.0
69	214454.0
70	179773.0
71	159528.5
72	139284.0
73	115255.5
74	91227.0
75	91227.0
76	77090.5
77	62954.0
78	51592.0
79	40230.0
80	31572.5
81	22915.0
82	22915.0
83	18070.5
84	13226.0
85	11252.0
86	9278.0
87	6859.0
88	4440.0
89	4440.0
90	3138.5
91	1837.0
92	1262.0
93	687.0
94	494.5
95	302.0
96	302.0
97	182.0
98	62.0
99	38.5
100	15.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	9064156.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.09209737182642
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.58272756368955	35.7595063323746
2	11.191213165905541	10.31652973903568
3	4.009653048672011	5.54439956239893
4	1.9182305041208652	3.5366106871018634
5	1.1273085547739965	2.5980007837367984
6	0.7236858111799692	2.0013718125309796
7	0.5429202425933084	1.7517032880722476
8	0.37642986288477404	1.3880353514998618
9	0.31314106485916	1.2989995607345235
>10	1.9345974847534444	16.860858053905996
>50	0.16974306569281697	5.395403760509692
>100	0.1011421639215601	8.694536475373868
>500	0.006697095704847215	2.0821817940805185
>1k	0.002414738093573674	1.7804085430451195
>5k	0.0	0.0
>10k+	9.563315449824449E-5	0.9914542555991178
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	30009	0.3310732957376285	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	29465	0.3250716338068321	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	19090	0.2106097909171025	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11406	0.125836316144603	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.4129867138209006E-5	0.0	0.0	1.1032466784552252E-5	1.1032466784552252E-5
2	2.978766031829108E-4	0.0	0.0	1.1032466784552252E-5	1.1032466784552252E-5
3	4.5233113816664234E-4	0.0	0.0	1.1032466784552252E-5	1.1032466784552252E-5
4	4.6336360495119455E-4	0.0	0.0	5.516233392276126E-5	1.1032466784552252E-5
5	0.0016879674180364946	0.0	0.0	7.722726749186576E-5	1.1032466784552252E-5
6	0.002316818024755973	0.0	0.0	8.825973427641801E-5	3.309740035365675E-5
7	0.00254849982723157	0.0	0.0	9.929220106097027E-5	4.4129867138209006E-5
8	0.003420064703211198	0.0	0.0	1.1032466784552252E-4	6.61948007073135E-5
9	0.0035634867714103772	0.0	0.0	1.2135713463007477E-4	6.61948007073135E-5
10	0.005174226921955006	0.0	0.0	1.5445453498373152E-4	6.61948007073135E-5
11	0.0055824281929834395	0.0	0.0	1.7651946855283602E-4	6.61948007073135E-5
12	0.0058472073958126935	0.0	0.0	1.875519353373883E-4	1.1032466784552252E-4
13	0.006255408666841127	0.0	0.0	2.4271426926014954E-4	1.2135713463007477E-4
14	0.00710490860925165	0.0	0.0	2.8684413639835856E-4	1.6548700176828379E-4
15	0.0077227267491865766	0.0	0.0	3.199415367520153E-4	1.7651946855283602E-4
16	0.008197122820922323	0.0	0.0	3.5303893710567205E-4	2.4271426926014954E-4
17	0.008461902023751577	0.0	0.0	3.5303893710567205E-4	2.64779202829254E-4
18	0.008792876027288144	0.0	0.0	3.5303893710567205E-4	2.978766031829108E-4
19	0.009145914964393816	0.0	0.0	3.5303893710567205E-4	3.0890906996746304E-4
20	0.011837836859824567	0.0	0.0	5.29558405658508E-4	3.0890906996746304E-4
21	0.017188583250332408	0.0	0.0	8.936298095487324E-4	3.199415367520153E-4
22	0.017585752054576288	0.0	0.0	0.0010039544773942548	3.199415367520153E-4
23	0.01812634292701935	0.0	0.0	0.0011032466784552252	4.412986713820901E-4
24	0.018887583135153454	0.0	0.0	0.0012907986137926134	4.412986713820901E-4
25	0.01935094674010465	1.1032466784552252E-5	0.0	0.001445253148776345	4.5233113816664234E-4
26	0.022329712771933758	1.1032466784552252E-5	0.0	0.0019527466208657486	4.6336360495119455E-4
27	0.02298062831222234	1.1032466784552252E-5	0.0	0.0026367595615079883	4.743960717357468E-4
28	0.024227297058876746	1.1032466784552252E-5	0.0	0.004578473715589185	4.743960717357468E-4
29	0.024470011328136896	1.1032466784552252E-5	0.0	0.008561194224812548	4.854285385202991E-4
30	0.02488924506594988	2.2064933569104503E-5	0.0	0.013989167882812256	4.854285385202991E-4
31	0.025793907342283165	2.2064933569104503E-5	0.0	0.02965527071687645	4.854285385202991E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	19725	0.0	14.302916	1
TAATCCC	4790	0.0	14.097077	5
TTAATCC	5205	0.0	13.435159	4
TTTAATC	5625	0.0	12.366222	3
CCTTTAA	5760	0.0	12.365451	1
TATACTG	1005	0.0	12.149254	5
TAGTACT	655	0.0	11.5801525	4
TCGTTAC	2385	0.0	11.402516	23
CTTTAAT	6190	0.0	11.267367	2
GGCGGGT	1075	0.0	11.013954	33
ATGGTTG	9510	0.0	10.971608	32
GGGTAAG	6840	0.0	10.8728075	1
TTAGTAC	375	1.8189894E-12	10.8533325	3
TTTATAC	1060	0.0	10.820754	3
GCACTCG	3165	0.0	10.813586	13
TACGGAT	4675	0.0	10.763637	27
ACGGATG	4675	0.0	10.763637	28
GTATCAA	26175	0.0	10.736008	2
CTCGTTA	2615	0.0	10.682601	22
GGCGAAT	1005	0.0	10.676618	37
>>END_MODULE