FastQCFastQC Report
Thu 9 Feb 2017
SRR2727581.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727581.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10192763
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT2264322.2214977430555387No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2095282.055654585513271No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1355701.3300613386183904No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT853360.837221467819864No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGT353470.346785263230392No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT288170.28272020059722763No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT255330.2505012625134127No Hit
ATCTAGTAGCTGGTTCCCTCCGAAGTTTCCCTCAGGATAGCTG200510.19671800472550968No Hit
TATCAACGCAGAGTACGGGTCATCTCCCCCAGATCTTGACTAT190760.18715239430172173No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT182180.17873465712878833No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTG166030.16289008191400114No Hit
ATACTAAAGTTTGATTAGCCAATAGATTCCTGTGGGGAAAAAA164070.16096714894675762No Hit
GGTATCAACGCAGAGTACGGGTCATCTCCCCCAGATCTTGACT155220.15228451794670395No Hit
CCTCTAATCATTCGCTTTACCGGATAAAACTGCGTACGTCGGG154330.15141134940545561No Hit
GATCTTGACTATATACTAAAGTTTGATTAGCCAATAGATTCCT148110.1453089804992032No Hit
GTATCAACGCAGAGTACGGGTCATCTCCCCCAGATCTTGACTA146820.1440433766585174No Hit
GGGTATAGGGGCGAAAGACTAATCGAACCATCTAGTAGCTGGT144930.14218911986867547No Hit
GAGTACGGGTCATCTCCCCCAGATCTTGACTATATACTAAAGT140520.13786252069237753No Hit
CTTCTTACCCATTTAAAGTTTGAGAATAGGTTGAGATCGTTTC125620.12324430578833237No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT118930.11668082540524097No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118360.11612160510354258No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116230.11403188713403814No Hit
CCCCCAGATCTTGACTATATACTAAAGTTTGATTAGCCAATAG114800.11262893093854924No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCG108740.10668353615207182No Hit
GGTTGAGATCGTTTCGGCCCCAAGACCTCTAATCATTCGCTTT108530.10647750761986716No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAG108500.10644807497240934No Hit
CATTTAAAGTTTGAGAATAGGTTGAGATCGTTTCGGCCCCAAG103560.10160149902435679No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACACA59150.027.3043124
TATACAC59700.027.1457273
GAGACTA61100.025.88788824
GCGAATC59500.025.71344633
ATCTCCG64100.024.90717710
TAAGGCG64350.024.52292329
CGAGACT65200.024.51533723
ACATCTC66500.024.4812038
GCCCACG65350.024.34582918
GGCGAAT63400.024.33596232
CACATCT67100.024.2347267
CACGAGA66300.023.91327321
AGGCGAA66000.023.79772631
ACGAGAC68000.023.58750222
CGAATCT65450.023.48892234
CCGAGCC69850.023.38654114
TTATACA70550.023.364282
CTCCGAG68850.023.26942812
TACACAT70450.023.2136275
AAGGCGA67850.023.2033930
Produced by FastQC (version 0.11.5)