##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727581.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10192763
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.13477356434168	33.0	31.0	34.0	30.0	34.0
2	32.34574943025753	34.0	31.0	34.0	31.0	34.0
3	32.35080144608484	34.0	31.0	34.0	30.0	34.0
4	35.923314021919275	37.0	35.0	37.0	35.0	37.0
5	35.88757974653193	37.0	35.0	37.0	35.0	37.0
6	35.947137297315756	37.0	35.0	37.0	35.0	37.0
7	35.862117268889705	37.0	35.0	37.0	35.0	37.0
8	35.87751368299253	37.0	35.0	37.0	35.0	37.0
9	37.532372625557954	39.0	37.0	39.0	35.0	39.0
10	37.47532185335811	39.0	37.0	39.0	35.0	39.0
11	37.5275832470548	39.0	37.0	39.0	35.0	39.0
12	37.42263702197334	39.0	37.0	39.0	34.0	39.0
13	37.406005712092	39.0	37.0	39.0	34.0	39.0
14	38.50405420002408	40.0	38.0	41.0	34.0	41.0
15	38.49364298963882	40.0	38.0	41.0	34.0	41.0
16	38.37376950685501	40.0	38.0	41.0	34.0	41.0
17	38.45538349120842	40.0	38.0	41.0	34.0	41.0
18	38.44587193874713	40.0	38.0	41.0	34.0	41.0
19	38.48585834871271	40.0	38.0	41.0	34.0	41.0
20	38.41509588715052	40.0	38.0	41.0	34.0	41.0
21	38.29079269281548	40.0	38.0	41.0	33.0	41.0
22	38.14310516196639	40.0	38.0	41.0	33.0	41.0
23	38.275691095731354	40.0	38.0	41.0	33.0	41.0
24	38.25656124840732	40.0	38.0	41.0	33.0	41.0
25	38.250820116194205	40.0	38.0	41.0	33.0	41.0
26	38.03012392223777	40.0	37.0	41.0	33.0	41.0
27	37.99008816353329	40.0	37.0	41.0	33.0	41.0
28	37.8315287032574	40.0	37.0	41.0	33.0	41.0
29	37.71831514183151	40.0	37.0	41.0	33.0	41.0
30	37.5431914781105	40.0	36.0	41.0	33.0	41.0
31	37.307673787764905	39.0	35.0	41.0	32.0	41.0
32	37.07785906529956	39.0	35.0	41.0	32.0	41.0
33	36.872936219551065	39.0	35.0	41.0	31.0	41.0
34	36.696519089083104	39.0	35.0	41.0	31.0	41.0
35	36.487986231015086	39.0	35.0	41.0	30.0	41.0
36	36.30950822657213	39.0	35.0	41.0	30.0	41.0
37	36.11578725022842	39.0	35.0	41.0	30.0	41.0
38	35.88650516057324	38.0	35.0	41.0	28.0	41.0
39	35.654561672826105	38.0	35.0	41.0	27.0	41.0
40	35.36206904840228	38.0	35.0	41.0	25.0	41.0
41	35.09779536716394	38.0	35.0	40.0	24.0	41.0
42	34.76020162540814	38.0	34.0	40.0	23.0	41.0
43	34.38561330230086	38.0	33.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	6.0
10	14.0
11	16.0
12	46.0
13	48.0
14	82.0
15	151.0
16	258.0
17	537.0
18	1100.0
19	2247.0
20	4364.0
21	7993.0
22	13099.0
23	20479.0
24	31327.0
25	46330.0
26	66456.0
27	91387.0
28	121950.0
29	156970.0
30	195785.0
31	241677.0
32	301569.0
33	385895.0
34	504966.0
35	650318.0
36	881590.0
37	1530824.0
38	1888831.0
39	3046447.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.54343988965504	21.122604341923775	14.480205220115488	21.853750548305694
2	19.8677336066776	25.269644746964097	32.31782196839071	22.544799677967596
3	18.74916546180854	26.27231693702679	32.82171870375088	22.156798897413783
4	12.932518886194059	18.74824323885486	34.5597950231944	33.75944285175668
5	13.410711109441081	38.29493533794517	33.30440431117647	14.989949241437284
6	29.500008976957474	38.50727226758829	13.699778951006708	18.292939804447528
7	26.35283484958887	32.774528358993535	21.710962964605375	19.16167382681222
8	28.204756649399187	34.687307062864114	17.951707500704174	19.156228787032525
9	25.86691165094293	13.576652375808207	21.50414956180184	39.05228641144702
10	18.95234883809228	29.360900474189382	29.935533672273163	21.751217015445174
11	31.303239366990088	24.759478857695406	21.36129330192412	22.575988473390385
12	21.319165372529508	30.872345408207764	28.869002448109505	18.93948677115322
13	32.72136318680224	20.610172138800834	26.204572793461402	20.463891880935524
14	20.641311879811195	24.426556371417643	30.773226062452352	24.158905686318814
15	25.400355134324226	28.82630548753071	25.54095489123018	20.232384486914885
16	20.783805137036936	25.704051001676387	29.817538188614805	23.694605672671877
17	21.40037985774809	27.83941900738789	28.56201012424207	22.198191010621947
18	22.710289643740367	23.16342487311831	30.746295190028455	23.37999029311287
19	23.120845643129346	24.212276887042307	32.697395200889105	19.969482268939245
20	22.36015886958227	24.202456193673886	32.97747627409761	20.45990866264623
21	21.102550898122523	25.9301329776823	32.51233252455689	20.454983599638293
22	20.956623832026704	25.73056981703587	32.44571663247738	20.86708971846005
23	20.547009677356375	25.579953149111777	32.73105633869835	21.1419808348335
24	20.212370286643573	26.980309460741903	32.76279454354035	20.044525709074172
25	21.06076634961492	25.89856155784256	32.81569482190452	20.224977270638
26	19.58274709222612	27.15060675893278	33.31647169663417	19.950174452206923
27	21.05996185725107	26.0050586872274	33.42207603571279	19.51290341980874
28	19.678089248224452	26.022904682469317	33.41512993091275	20.883876138393486
29	19.930199495465555	26.598577834096602	32.73910126233682	20.732121408101023
30	19.456304438747374	26.796973499727205	32.44608944601185	21.300632615513575
31	19.51920200636471	26.33782419938539	33.31292996805675	20.830043826193155
32	19.550999076501633	27.12752175244337	32.18109751006671	21.140381660988293
33	19.52341087495118	27.70725660941984	32.582725606393474	20.186606909235504
34	20.68465635863406	27.64385868679572	31.755540671356723	19.91594428321349
35	20.45638655583378	28.973880781884166	31.64536446103966	18.92436820124239
36	19.919770527383008	28.629263723683167	31.41934135032866	20.031624398605167
37	20.107256491689252	27.96514546644516	30.843295385166908	21.08430265669868
38	19.36582848046207	27.964027025841766	32.52871669830839	20.141427795387767
39	19.983374478539332	28.7867578202299	30.37494347705328	20.854924224177488
40	19.54269906991853	28.92298192354713	31.69836284822869	19.835956158305653
41	19.901610583901537	28.49647342923602	30.994147514270665	20.60776847259178
42	19.74039816289263	28.882453167997724	30.456746615221014	20.920402053888626
43	20.152916338778798	28.683861284717405	30.83323923061882	20.329983145884977
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1095.0
1	2422.0
2	3749.0
3	8913.0
4	14077.0
5	14077.0
6	19445.0
7	24813.0
8	23082.0
9	21351.0
10	32338.0
11	43325.0
12	43325.0
13	109545.0
14	175765.0
15	280792.5
16	385820.0
17	327357.0
18	268894.0
19	268894.0
20	317849.0
21	366804.0
22	264567.5
23	162331.0
24	181825.0
25	201319.0
26	201319.0
27	240708.0
28	280097.0
29	330915.5
30	381734.0
31	425819.5
32	469905.0
33	469905.0
34	505230.5
35	540556.0
36	544255.0
37	547954.0
38	560801.5
39	573649.0
40	573649.0
41	585449.0
42	597249.0
43	590963.5
44	584678.0
45	566673.0
46	548668.0
47	548668.0
48	560422.5
49	572177.0
50	567852.5
51	563528.0
52	539350.5
53	515173.0
54	515173.0
55	472086.0
56	428999.0
57	368257.5
58	307516.0
59	282971.5
60	258427.0
61	258427.0
62	253510.5
63	248594.0
64	225035.5
65	201477.0
66	195497.5
67	189518.0
68	189518.0
69	198420.0
70	207322.0
71	191627.5
72	175933.0
73	138794.0
74	101655.0
75	101655.0
76	90021.5
77	78388.0
78	67032.0
79	55676.0
80	46546.0
81	37416.0
82	37416.0
83	30309.0
84	23202.0
85	19218.5
86	15235.0
87	12445.5
88	9656.0
89	9656.0
90	7318.5
91	4981.0
92	3804.5
93	2628.0
94	1764.5
95	901.0
96	901.0
97	639.5
98	378.0
99	264.0
100	150.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1.0192763E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.580468005360775
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.01341689032587	11.774356867013944
2	12.676522419458205	4.203653487901306
3	5.123170219311492	2.54833679721934
4	2.7339803387706176	1.8132269413708653
5	1.6079290324568416	1.3330107938770686
6	1.034115763124036	1.0287673994590392
7	0.7159199707600342	0.83092017187099
8	0.5191225205525934	0.6885835474307613
9	0.41579268801160557	0.6204633624395443
>10	2.9064876169832674	9.851878433847244
>50	0.5152582297251007	6.074210592455269
>100	0.5976664268757365	21.15441361740954
>500	0.08897083944972684	10.071287659482163
>1k	0.046951858359315785	14.371697557244653
>5k	0.003090502069219137	3.50270403112676
>10k+	0.0016046837667099366	10.132488739851562
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	226432	2.2214977430555387	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	209528	2.055654585513271	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	135570	1.3300613386183904	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	85336	0.837221467819864	No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGT	35347	0.346785263230392	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	28817	0.28272020059722763	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	25533	0.2505012625134127	No Hit
ATCTAGTAGCTGGTTCCCTCCGAAGTTTCCCTCAGGATAGCTG	20051	0.19671800472550968	No Hit
TATCAACGCAGAGTACGGGTCATCTCCCCCAGATCTTGACTAT	19076	0.18715239430172173	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	18218	0.17873465712878833	No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTG	16603	0.16289008191400114	No Hit
ATACTAAAGTTTGATTAGCCAATAGATTCCTGTGGGGAAAAAA	16407	0.16096714894675762	No Hit
GGTATCAACGCAGAGTACGGGTCATCTCCCCCAGATCTTGACT	15522	0.15228451794670395	No Hit
CCTCTAATCATTCGCTTTACCGGATAAAACTGCGTACGTCGGG	15433	0.15141134940545561	No Hit
GATCTTGACTATATACTAAAGTTTGATTAGCCAATAGATTCCT	14811	0.1453089804992032	No Hit
GTATCAACGCAGAGTACGGGTCATCTCCCCCAGATCTTGACTA	14682	0.1440433766585174	No Hit
GGGTATAGGGGCGAAAGACTAATCGAACCATCTAGTAGCTGGT	14493	0.14218911986867547	No Hit
GAGTACGGGTCATCTCCCCCAGATCTTGACTATATACTAAAGT	14052	0.13786252069237753	No Hit
CTTCTTACCCATTTAAAGTTTGAGAATAGGTTGAGATCGTTTC	12562	0.12324430578833237	No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT	11893	0.11668082540524097	No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11836	0.11612160510354258	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11623	0.11403188713403814	No Hit
CCCCCAGATCTTGACTATATACTAAAGTTTGATTAGCCAATAG	11480	0.11262893093854924	No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCG	10874	0.10668353615207182	No Hit
GGTTGAGATCGTTTCGGCCCCAAGACCTCTAATCATTCGCTTT	10853	0.10647750761986716	No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAG	10850	0.10644807497240934	No Hit
CATTTAAAGTTTGAGAATAGGTTGAGATCGTTTCGGCCCCAAG	10356	0.10160149902435679	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.158394146905996E-4	0.0	0.0	2.9432647457809035E-5	0.0
2	0.0027764797435199857	0.0	0.0	9.810882485936345E-5	0.0
3	0.0037673788745995565	9.810882485936346E-6	0.0	1.3735235480310883E-4	0.0
4	0.004091137996635456	9.810882485936346E-6	0.0	1.962176497187269E-4	0.0
5	0.014637836669017027	9.810882485936346E-6	0.0	5.199767717546263E-4	0.0
6	0.02031833762837417	9.810882485936346E-6	0.0	7.456270689311622E-4	0.0
7	0.022888788839689492	9.810882485936346E-6	0.0	8.143032463327166E-4	0.0
8	0.02928548422051999	9.810882485936346E-6	0.0	0.0012263603107420431	0.0
9	0.030963145125615106	9.810882485936346E-6	0.0	0.0013440909005732794	0.0
10	0.04749448211441785	9.810882485936346E-6	0.0	0.002266313854251296	0.0
11	0.05067320803986122	9.810882485936346E-6	0.0	0.002482153268941895	0.0
12	0.05300819807151407	9.810882485936346E-6	0.0	0.00273723621357624	0.0
13	0.05718763401052296	9.810882485936346E-6	0.0	0.003129671513013694	0.0
14	0.06572310177328758	9.810882485936346E-6	0.0	0.00386548769945892	0.0
15	0.07113870890552444	9.810882485936346E-6	0.0	0.004326599176297928	0.0
16	0.07523965778464584	9.810882485936346E-6	0.0	0.004748467123193191	0.0
17	0.07658374868521911	9.810882485936346E-6	0.0	0.004925063007940046	0.0
18	0.07859497959483606	9.810882485936346E-6	0.0	0.005121280657658772	0.0
19	0.0825193325892106	9.810882485936346E-6	0.0	0.0056314465469274625	0.0
20	0.10537868878144228	9.810882485936346E-6	0.0	0.007623055691572541	0.0
21	0.15333428237269914	9.810882485936346E-6	0.0	0.012518686052054777	0.0
22	0.15703298506989713	9.810882485936346E-6	0.0	0.013725424597824947	0.0
23	0.16031963070268582	9.810882485936346E-6	0.0	0.014726134611390454	0.0
24	0.16643181049142416	9.810882485936346E-6	0.0	0.016492093458858995	0.0
25	0.17031691995585496	9.810882485936346E-6	0.0	0.01774788641705885	0.0
26	0.19347060262266472	9.810882485936346E-6	0.0	0.021162073522164697	0.0
27	0.19962202594134681	9.810882485936346E-6	0.0	0.02512567004648298	0.0
28	0.20827522429394268	9.810882485936346E-6	0.0	0.03550558371660363	0.0
29	0.2094427193097691	9.810882485936346E-6	0.0	0.06422203675293932	0.0
30	0.21212109022842973	9.810882485936346E-6	0.0	0.09957064634976796	0.0
31	0.2170853967663135	9.810882485936346E-6	0.0	0.17537933531859812	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATACACA	5915	0.0	27.304312	4
TATACAC	5970	0.0	27.145727	3
GAGACTA	6110	0.0	25.887888	24
GCGAATC	5950	0.0	25.713446	33
ATCTCCG	6410	0.0	24.907177	10
TAAGGCG	6435	0.0	24.522923	29
CGAGACT	6520	0.0	24.515337	23
ACATCTC	6650	0.0	24.481203	8
GCCCACG	6535	0.0	24.345829	18
GGCGAAT	6340	0.0	24.335962	32
CACATCT	6710	0.0	24.234726	7
CACGAGA	6630	0.0	23.913273	21
AGGCGAA	6600	0.0	23.797726	31
ACGAGAC	6800	0.0	23.587502	22
CGAATCT	6545	0.0	23.488922	34
CCGAGCC	6985	0.0	23.386541	14
TTATACA	7055	0.0	23.36428	2
CTCCGAG	6885	0.0	23.269428	12
TACACAT	7045	0.0	23.213627	5
AAGGCGA	6785	0.0	23.20339	30
>>END_MODULE