FastQCFastQC Report
Thu 9 Feb 2017
SRR2727580.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727580.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1252940
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT113020.9020384056698645No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT103830.8286909189586094No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52690.4205309112966303No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT48210.3847750091784124No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT16300.13009401886762334No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15860.12658227848101267No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA30200.022.1142371
CGACGCG500.00703522718.517
ACGGATA609.237449E-418.514
GTATCAA40150.016.679952
GTAAGAC1251.6579543E-716.2799993
CGATAAC700.002592668615.8571421
AATCGGG855.364629E-415.23529425
ACGTCGG1101.45199665E-515.13636415
GACCGGT750.004104679514.86
TGCGTAC908.2747464E-414.38888910
TATACAC3000.014.1833333
GTAGCAC1702.4365363E-814.1470593
CGCAATC800.006299037513.87500122
TAAGGTG1753.565401E-813.7428575
ATAGACG950.001245182913.6315793
CGTACGT950.001245182913.63157912
GTACGTC950.001245182913.63157913
TACGTCG950.001245182913.63157914
GGGTAAG6850.013.503651
GTAAGAG6950.013.3093533
Produced by FastQC (version 0.11.5)