FastQCFastQC Report
Thu 9 Feb 2017
SRR2727579.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727579.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2977277
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT135340.4545764468673892No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT134040.4502100409199412No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT93990.31569115000048703No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48260.16209442386449094No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA42200.024.7251171
GTATCAA66500.015.5789472
GTCTTAC2950.014.4237291
GGGTATA3750.013.8133331
CGCGTAT1501.3073804E-613.5666677
GTCGTAC1102.4584177E-413.4545451
TTAATCC10250.013.175614
TAATCCC10300.012.7524285
ACCGCTA2653.274181E-1112.5660388
TAGTACT1851.0408239E-612.04
TTTAATC11550.011.8528143
TACGTAT1100.003747446211.77272734
ATTTCGC1100.003747446211.77272718
TACACTT4150.011.5903625
TTACACT4950.011.5858584
TAGAGTC5600.011.5625015
TTGCACG4250.011.31764726
ATACGCG1801.0044212E-511.3055554
TATACAC3600.011.3055553
TAGCGCG2952.0918378E-1011.2881364
Produced by FastQC (version 0.11.5)