##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727579.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2977277
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.23337197042801	33.0	31.0	34.0	31.0	34.0
2	32.45774377056619	34.0	31.0	34.0	31.0	34.0
3	32.52048465762507	34.0	31.0	34.0	31.0	34.0
4	36.07265901022982	37.0	35.0	37.0	35.0	37.0
5	35.955305468721924	37.0	35.0	37.0	35.0	37.0
6	35.966053880777636	37.0	35.0	37.0	35.0	37.0
7	35.89592537073305	37.0	35.0	37.0	35.0	37.0
8	35.87136568078818	37.0	35.0	37.0	35.0	37.0
9	37.54734275648521	39.0	37.0	39.0	35.0	39.0
10	37.4616077039523	39.0	37.0	39.0	35.0	39.0
11	37.503837231134355	39.0	37.0	39.0	35.0	39.0
12	37.35671857203747	39.0	37.0	39.0	34.0	39.0
13	37.34292375213996	39.0	37.0	39.0	34.0	39.0
14	38.56311656590905	40.0	38.0	41.0	34.0	41.0
15	38.51859568323673	40.0	38.0	41.0	34.0	41.0
16	38.446359542629054	40.0	38.0	41.0	34.0	41.0
17	38.37460672957202	40.0	38.0	41.0	33.0	41.0
18	38.2819529388767	40.0	38.0	41.0	33.0	41.0
19	38.23928038942967	40.0	38.0	41.0	33.0	41.0
20	38.11802630390118	40.0	38.0	41.0	33.0	41.0
21	37.99505454144845	40.0	37.0	41.0	33.0	41.0
22	37.870844399093535	40.0	37.0	41.0	32.0	41.0
23	38.21669733786947	40.0	38.0	41.0	33.0	41.0
24	38.31724659814992	40.0	38.0	41.0	34.0	41.0
25	38.35932665989762	40.0	38.0	41.0	34.0	41.0
26	38.24329076535371	40.0	38.0	41.0	33.0	41.0
27	38.134869882782155	40.0	38.0	41.0	33.0	41.0
28	38.034563797725234	40.0	38.0	41.0	33.0	41.0
29	37.94021550564492	40.0	38.0	41.0	33.0	41.0
30	37.84074877816206	40.0	37.0	41.0	33.0	41.0
31	37.72738008589728	40.0	37.0	41.0	32.0	41.0
32	37.592473592480644	40.0	37.0	41.0	32.0	41.0
33	37.48576031051192	40.0	37.0	41.0	32.0	41.0
34	37.36639251235273	40.0	37.0	41.0	31.0	41.0
35	37.24652560040601	40.0	36.0	41.0	31.0	41.0
36	37.14968912869041	40.0	36.0	41.0	31.0	41.0
37	37.04535217918924	40.0	36.0	41.0	30.0	41.0
38	36.905306761849836	40.0	36.0	41.0	30.0	41.0
39	36.78707725213341	40.0	36.0	41.0	30.0	41.0
40	36.6604743193193	39.0	35.0	41.0	30.0	41.0
41	36.533355142971246	39.0	35.0	41.0	30.0	41.0
42	36.38345138863465	39.0	35.0	41.0	29.0	41.0
43	36.22702959785065	39.0	35.0	41.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	3.0
11	2.0
12	7.0
13	10.0
14	17.0
15	43.0
16	83.0
17	141.0
18	307.0
19	564.0
20	1124.0
21	1823.0
22	3018.0
23	4856.0
24	7179.0
25	10698.0
26	15203.0
27	21072.0
28	28499.0
29	38189.0
30	49740.0
31	63207.0
32	80813.0
33	103332.0
34	133653.0
35	173562.0
36	235442.0
37	356063.0
38	601794.0
39	1046833.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.875646773880966	19.09093443438417	12.601447564334794	26.43197122740007
2	20.235671722852793	21.68897956085376	32.97869160309907	25.09665711319437
3	19.716103002844545	22.889270968069145	30.15705290438209	27.237573124704216
4	15.485962508695025	16.30983613550234	33.16946995526449	35.03473140053814
5	15.025138742548979	35.96195449734774	33.563386947200414	15.449519812902865
6	34.9541544169387	35.20062123880311	14.318184031919099	15.527040312339095
7	29.490269128468732	30.482450910681138	20.283567837322494	19.74371212352764
8	27.48346895502165	33.81317895513249	18.853368363104945	19.84998372674091
9	26.916910989471255	14.27750928113172	18.944894949311063	39.86068478008596
10	17.36197203014701	27.221854063293407	32.095502030882585	23.320671875677
11	35.942070556417825	22.075641601369306	20.652764254048243	21.329523588164623
12	22.042759205811215	25.820943096661814	28.612990998150323	23.523306699376644
13	31.260578038254415	20.234193862378273	23.482027369304234	25.02320073006307
14	22.56548517319685	21.198229120098667	25.11764944948018	31.118636257224303
15	25.408284146889926	28.014289567279093	22.12011176655716	24.457314519273822
16	24.61346391350217	26.08292745350869	24.307748321704697	24.99586031128444
17	24.204835492297157	26.22698526203642	25.017121349474703	24.551057896191722
18	24.62616007848783	24.956193192638775	25.847846874845708	24.56979985402769
19	25.608567828925562	24.68859968353633	26.164142604131225	23.538689883406885
20	25.089603688202338	24.668581391654186	26.318948488837286	23.92286643130619
21	24.915552029589456	24.75369271989136	26.192389891837408	24.138365358681778
22	24.92777796624231	24.65910964952203	25.82184996558936	24.591262418646302
23	24.659814992021232	24.672208867364372	25.90309870394995	24.76487743666444
24	24.216624788355265	25.082751789638653	26.094280108972058	24.606343313034024
25	24.680840916045096	24.77545757415249	26.090954922904388	24.452746586898026
26	24.240169792733425	25.219588234484057	26.161489172824698	24.378752799957816
27	24.755640808698686	25.123997531973007	26.023980973218148	24.096380686110162
28	24.36575434532964	24.935738260161884	26.14452736510577	24.553980029402705
29	24.289039951606785	25.24091644815044	26.06586488257559	24.404178717667182
30	24.466215269859003	25.226238606619404	25.889495670036748	24.418050453484845
31	24.822043766837954	24.452041244398824	26.101367121702147	24.624547867061075
32	24.143840159985114	25.151841766822503	25.87139187922387	24.832926193968515
33	24.18481720041501	24.711304994463063	26.276460000194806	24.827417804927123
34	24.78385450866681	24.67560122890816	26.276292061504524	24.264252200920506
35	24.627940228604864	25.341175846251456	26.021025252269105	24.009858672874575
36	24.330252106203083	25.132159352320933	26.196655534570684	24.340933006905303
37	24.80074914090963	24.957838991803584	25.89507123455426	24.346340632732527
38	24.336969653814542	25.07438844286239	26.348069057732953	24.240572845590115
39	24.424096246335157	25.288611036191792	25.985153548023916	24.30213916944913
40	24.621928023492607	24.990587036409444	26.163638788060368	24.223846152037584
41	24.41281076634791	25.066092271562233	26.095153390161546	24.42594357192831
42	24.510181618976	25.0652525781108	25.789437798364073	24.635128004549124
43	24.465677866050083	24.681344732115956	25.939474224266	24.913503177567957
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	159.0
1	269.0
2	379.0
3	568.5
4	758.0
5	758.0
6	1060.5
7	1363.0
8	1313.5
9	1264.0
10	2014.0
11	2764.0
12	2764.0
13	6663.0
14	10562.0
15	16559.0
16	22556.0
17	20452.5
18	18349.0
19	18349.0
20	22568.0
21	26787.0
22	22801.0
23	18815.0
24	23075.5
25	27336.0
26	27336.0
27	34233.5
28	41131.0
29	48320.5
30	55510.0
31	64803.0
32	74096.0
33	74096.0
34	84716.0
35	95336.0
36	106484.5
37	117633.0
38	130602.0
39	143571.0
40	143571.0
41	154865.5
42	166160.0
43	178565.5
44	190971.0
45	199762.5
46	208554.0
47	208554.0
48	220435.5
49	232317.0
50	237541.5
51	242766.0
52	239605.5
53	236445.0
54	236445.0
55	229805.0
56	223165.0
57	211376.0
58	199587.0
59	184358.0
60	169129.0
61	169129.0
62	150472.0
63	131815.0
64	113639.0
65	95463.0
66	82154.5
67	68846.0
68	68846.0
69	59145.0
70	49444.0
71	43119.0
72	36794.0
73	30359.5
74	23925.0
75	23925.0
76	19948.5
77	15972.0
78	13286.0
79	10600.0
80	8542.5
81	6485.0
82	6485.0
83	5290.0
84	4095.0
85	3491.0
86	2887.0
87	2319.0
88	1751.0
89	1751.0
90	1339.5
91	928.0
92	677.0
93	426.0
94	352.0
95	278.0
96	278.0
97	173.5
98	69.0
99	52.5
100	36.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2977277.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.20352195353122
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.56995630765451	45.845188300858425
2	10.19801243891005	11.46328431985331
3	3.139228655551958	5.2930711997840625
4	1.4030247764054555	3.1541973528820897
5	0.8248621379650951	2.318007863987896
6	0.5182532312059779	1.7476594114544202
7	0.37789753599299686	1.486742072225743
8	0.28256299184389494	1.270482825228305
9	0.2186211343750031	1.1058549952816248
>10	1.2862487647389884	13.567568970635119
>50	0.11698425653005227	4.546762718212657
>100	0.06225435990565801	6.017130736833103
>500	0.0015551037694985159	0.5832759464549764
>1k	3.588701005948686E-4	0.37925878779755107
>5k	5.9811683432478105E-5	0.31595934643761586
>10k+	1.1962336686495621E-4	0.905555152073252
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	13534	0.4545764468673892	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	13404	0.4502100409199412	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	9399	0.31569115000048703	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4826	0.16209442386449094	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.358773805729195E-5	0.0	0.0	0.0	0.0
2	3.694651186302114E-4	0.0	0.0	0.0	0.0
3	5.709915469739632E-4	0.0	0.0	0.0	0.0
4	6.045792850312551E-4	0.0	0.0	6.71754761145839E-5	0.0
5	0.001981676545380225	0.0	0.0	1.0076321417187584E-4	3.358773805729195E-5
6	0.0028885454729271074	0.0	0.0	1.0076321417187584E-4	6.71754761145839E-5
7	0.0030900719012708594	0.0	0.0	1.0076321417187584E-4	6.71754761145839E-5
8	0.0037954144004739903	0.0	0.0	1.0076321417187584E-4	6.71754761145839E-5
9	0.00396335309076045	0.0	0.0	1.6793869028645974E-4	6.71754761145839E-5
10	0.005945029636140675	0.0	0.0	2.687019044583356E-4	6.71754761145839E-5
11	0.006482433445057346	0.0	0.0	2.687019044583356E-4	6.71754761145839E-5
12	0.00671754761145839	0.0	0.0	2.687019044583356E-4	6.71754761145839E-5
13	0.007087012730088601	0.0	0.0	3.0228964251562753E-4	1.0076321417187584E-4
14	0.008296171300151112	0.0	0.0	4.7022833280208726E-4	2.0152642834375168E-4
15	0.00896792606129695	3.358773805729195E-5	0.0	6.381670230885471E-4	2.0152642834375168E-4
16	0.009505329870213622	3.358773805729195E-5	0.0	7.725179753177148E-4	2.0152642834375168E-4
17	0.009639680822442789	3.358773805729195E-5	0.0	8.061057133750067E-4	2.0152642834375168E-4
18	0.009874794988843832	3.358773805729195E-5	0.0	8.732811894895906E-4	2.3511416640104363E-4
19	0.01051296201193238	3.358773805729195E-5	0.0	8.732811894895906E-4	2.3511416640104363E-4
20	0.013468682960974072	3.358773805729195E-5	0.0	0.0010748076178333423	2.3511416640104363E-4
21	0.020622871167177256	3.358773805729195E-5	0.0	0.0017129746409218893	2.3511416640104363E-4
22	0.02099233628580747	3.358773805729195E-5	0.0	0.0017801501170364733	2.3511416640104363E-4
23	0.02159691557083872	3.358773805729195E-5	0.0	0.0018137378550937653	3.0228964251562753E-4
24	0.022235082593927268	3.358773805729195E-5	0.0	0.001948088807322933	3.358773805729195E-4
25	0.022638135450614775	3.358773805729195E-5	0.0	0.001981676545380225	3.358773805729195E-4
26	0.025090040328797084	3.358773805729195E-5	0.0	0.00258625583041148	3.694651186302114E-4
27	0.025627444137713756	3.358773805729195E-5	0.0	0.003291598329614611	3.694651186302114E-4
28	0.026937365921948144	3.358773805729195E-5	0.0	0.004937397494421916	3.694651186302114E-4
29	0.027172480088349184	3.358773805729195E-5	0.0	0.008766399632953198	3.694651186302114E-4
30	0.027407594254750228	3.358773805729195E-5	0.0	0.014341964150463661	3.694651186302114E-4
31	0.027844234849495025	3.358773805729195E-5	0.0	0.025963321518286675	3.694651186302114E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	4220	0.0	24.725117	1
GTATCAA	6650	0.0	15.578947	2
GTCTTAC	295	0.0	14.423729	1
GGGTATA	375	0.0	13.813333	1
CGCGTAT	150	1.3073804E-6	13.566667	7
GTCGTAC	110	2.4584177E-4	13.454545	1
TTAATCC	1025	0.0	13.17561	4
TAATCCC	1030	0.0	12.752428	5
ACCGCTA	265	3.274181E-11	12.566038	8
TAGTACT	185	1.0408239E-6	12.0	4
TTTAATC	1155	0.0	11.852814	3
TACGTAT	110	0.0037474462	11.772727	34
ATTTCGC	110	0.0037474462	11.772727	18
TACACTT	415	0.0	11.590362	5
TTACACT	495	0.0	11.585858	4
TAGAGTC	560	0.0	11.562501	5
TTGCACG	425	0.0	11.317647	26
ATACGCG	180	1.0044212E-5	11.305555	4
TATACAC	360	0.0	11.305555	3
TAGCGCG	295	2.0918378E-10	11.288136	4
>>END_MODULE