##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727578.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1261589
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.229018325302455	31.0	31.0	33.0	30.0	34.0
2	31.500242154933183	31.0	31.0	34.0	30.0	34.0
3	31.498925561335746	31.0	31.0	34.0	30.0	34.0
4	35.28125958612512	37.0	35.0	37.0	33.0	37.0
5	34.940522626624045	35.0	35.0	37.0	32.0	37.0
6	35.11267457151259	36.0	35.0	37.0	32.0	37.0
7	35.08587345006971	36.0	35.0	37.0	32.0	37.0
8	35.06079079636871	36.0	35.0	37.0	32.0	37.0
9	36.67880744045802	38.0	35.0	39.0	32.0	39.0
10	36.37627309686435	38.0	35.0	39.0	32.0	39.0
11	36.523909133640196	38.0	35.0	39.0	32.0	39.0
12	36.24599612076516	38.0	35.0	39.0	32.0	39.0
13	36.30723952095334	38.0	35.0	39.0	32.0	39.0
14	37.25410256430581	39.0	36.0	40.0	32.0	41.0
15	37.18112871941655	39.0	36.0	40.0	32.0	41.0
16	37.069865859642086	39.0	36.0	40.0	31.0	41.0
17	36.91354474396971	38.0	36.0	40.0	31.0	41.0
18	36.771110084187484	38.0	36.0	40.0	31.0	41.0
19	36.64921539423695	38.0	36.0	40.0	30.0	41.0
20	36.461640835486044	38.0	35.0	40.0	30.0	41.0
21	36.297559664835376	38.0	35.0	40.0	30.0	41.0
22	36.15076225299999	38.0	34.0	40.0	29.0	41.0
23	36.50870846210612	38.0	35.0	40.0	30.0	41.0
24	36.5704092220208	38.0	35.0	40.0	30.0	41.0
25	36.58885262950137	38.0	35.0	40.0	30.0	41.0
26	36.396604599437694	38.0	35.0	40.0	30.0	41.0
27	36.23401044238655	38.0	35.0	40.0	29.0	41.0
28	36.05225869914845	38.0	35.0	40.0	29.0	41.0
29	35.914635431983	38.0	35.0	40.0	28.0	41.0
30	35.78483721719197	38.0	34.0	40.0	27.0	41.0
31	35.63917963774256	38.0	34.0	40.0	27.0	41.0
32	35.475595459377026	38.0	34.0	40.0	27.0	41.0
33	35.338913069153264	38.0	34.0	40.0	26.0	41.0
34	35.1541310204829	38.0	34.0	40.0	26.0	41.0
35	34.99842103886448	38.0	33.0	40.0	25.0	41.0
36	34.880920807014014	38.0	33.0	40.0	25.0	41.0
37	34.753815228255796	38.0	33.0	40.0	25.0	41.0
38	34.565230039260015	38.0	33.0	40.0	24.0	41.0
39	34.38661957261834	38.0	33.0	40.0	24.0	41.0
40	34.225444261165876	37.0	33.0	40.0	23.0	41.0
41	34.07584244948236	37.0	32.0	40.0	23.0	41.0
42	33.91734946959747	37.0	32.0	40.0	23.0	41.0
43	33.736466472044384	37.0	32.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	5.0
12	9.0
13	19.0
14	20.0
15	58.0
16	136.0
17	257.0
18	461.0
19	781.0
20	1444.0
21	2301.0
22	3483.0
23	5457.0
24	7674.0
25	10711.0
26	14449.0
27	18917.0
28	23917.0
29	30481.0
30	37562.0
31	46784.0
32	57565.0
33	71439.0
34	90974.0
35	117187.0
36	156880.0
37	211469.0
38	246684.0
39	104463.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.97743242846918	22.430759938458564	13.996872198473511	24.594935434598746
2	26.601611142773123	23.31702321437489	27.38958567330565	22.691779969546342
3	25.800320072543435	23.180132356892777	26.77076290297395	24.248784667589838
4	22.692414090484302	17.338689541522637	28.312310903154675	31.65658546483839
5	16.889018531391763	35.95021833576545	31.63787889716857	15.522884235674217
6	37.70586141762492	33.970254972102644	13.026825693629224	15.297057916643217
7	31.29521579531844	29.226792560810217	18.82189841541104	20.6560932284603
8	28.828564611771345	32.99045885783722	17.819670272965286	20.36130625742615
9	28.09092343068939	13.246469333515115	17.009263714252423	41.65334352154307
10	17.927470832418482	27.05611732505594	31.09277268587472	23.923639156650857
11	39.21031334293498	20.553444901628026	18.673197055459426	21.563044699977567
12	23.269543409145133	24.47936689365554	27.021874794406102	25.229214902793224
13	33.180695139225215	19.108996670072425	21.45904886615213	26.25125932455023
14	23.7685173222024	19.820242567111794	22.769063458860213	33.642176651825594
15	27.40797518050649	26.744288353814117	20.011509295023973	25.83622717065542
16	26.715911441840408	25.100963943090814	22.255187703760893	25.92793691130788
17	26.450690359538648	24.791512925366344	22.78087396132972	25.97692275376529
18	26.568161263295732	24.024622916020984	23.4206227226141	25.986593098069182
19	27.697055063098997	23.778504726975267	23.366009056832297	25.158431153093442
20	27.1154076327552	23.612127245878014	23.558068435916926	25.71439668544986
21	27.1335593446043	23.596274222429017	23.417531383041545	25.852635049925134
22	27.217342573532267	23.886463816662953	22.730857672348126	26.165335937456653
23	27.02774041308223	23.68544747932964	23.035949108624123	26.250862998964003
24	26.54287569089458	23.88051893286958	23.084538625495306	26.492066750740534
25	26.890056904427674	23.787303154989463	23.273189604538402	26.049450336044465
26	26.575057328496047	24.02311687879333	23.1084766909033	26.293349101807323
27	27.125870628231542	24.148038703571448	22.98720106151845	25.738889606678562
28	26.573709821502884	24.32361093826912	23.006303954774495	26.096375285453504
29	26.74626998174524	24.296581533288574	23.04712549015567	25.91002299481051
30	26.889184988137977	24.140349987198682	22.929020465460624	26.04144455920272
31	27.181356210303036	23.516374984246056	23.29498751178078	26.007281293670125
32	26.64988359917533	23.954631817493652	23.03206511787912	26.363419465451905
33	26.769019070394556	23.470004890657734	23.20922265492169	26.551753384026018
34	27.08084804163638	23.5941340642634	23.332796972706642	25.992220921393578
35	27.2057698664145	23.83438663463299	23.170065687002662	25.78977781194985
36	26.787487842712643	23.691867953826485	23.40461116893061	26.11603303453026
37	26.9869981428183	24.021135250862205	23.07241106255682	25.919455543762666
38	26.837662661928725	23.563696259241322	23.445274174077294	26.153366904752655
39	26.67294974829362	24.05339615358092	23.37924633141221	25.894407766713247
40	27.139345698163194	23.50321697478339	23.551410166068347	25.806027160985074
41	26.629750259395095	23.646766102114082	23.463663681278135	26.259819957212688
42	26.890770290482873	23.4745230023407	23.146920272767122	26.487786434409305
43	26.786615926422947	23.252897734523685	23.458907774243436	26.501578564809936
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	38.0
1	45.0
2	52.0
3	47.5
4	43.0
5	43.0
6	72.0
7	101.0
8	122.5
9	144.0
10	206.0
11	268.0
12	268.0
13	778.5
14	1289.0
15	1915.0
16	2541.0
17	2251.5
18	1962.0
19	1962.0
20	2711.0
21	3460.0
22	3455.5
23	3451.0
24	4336.5
25	5222.0
26	5222.0
27	6852.5
28	8483.0
29	10362.0
30	12241.0
31	14902.5
32	17564.0
33	17564.0
34	20899.5
35	24235.0
36	28659.5
37	33084.0
38	38833.5
39	44583.0
40	44583.0
41	50074.5
42	55566.0
43	63020.0
44	70474.0
45	78111.5
46	85749.0
47	85749.0
48	93956.5
49	102164.0
50	108384.5
51	114605.0
52	116159.0
53	117713.0
54	117713.0
55	115228.0
56	112743.0
57	107759.5
58	102776.0
59	95576.5
60	88377.0
61	88377.0
62	79424.5
63	70472.0
64	61554.0
65	52636.0
66	45414.5
67	38193.0
68	38193.0
69	32862.0
70	27531.0
71	24254.5
72	20978.0
73	17745.5
74	14513.0
75	14513.0
76	12554.0
77	10595.0
78	8812.5
79	7030.0
80	5611.0
81	4192.0
82	4192.0
83	3402.0
84	2612.0
85	2171.0
86	1730.0
87	1434.5
88	1139.0
89	1139.0
90	860.5
91	582.0
92	429.0
93	276.0
94	212.0
95	148.0
96	148.0
97	85.0
98	22.0
99	17.0
100	12.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1261589.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	67.5181728427792
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.77977643780017	57.9169377194235
2	7.896044379998776	10.662529784460252
3	2.441102104724749	4.94456261501033
4	1.1717725352398254	3.1646376226697637
5	0.6535661986869836	2.206379778357296
6	0.4388113811362886	1.777664560616315
7	0.29559936625819583	1.3970830372265801
8	0.20863114761106022	1.1269115107832754
9	0.17076255836809145	1.037661833787477
>10	0.8684503871419407	10.490552246908328
>50	0.053467731813582615	2.477120097770825
>100	0.02130939022961628	2.3893049259805217
>500	4.709206604659938E-4	0.18505098883272805
>1k	2.354603302329969E-4	0.22360327817287973
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1459	0.11564780606045234	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1354	0.10732496874972754	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	1.5853023448999634E-4	0.0
3	0.0	0.0	0.0	2.3779535173499452E-4	0.0
4	0.0	0.0	0.0	3.170604689799927E-4	0.0
5	3.9632558622499086E-4	0.0	0.0	7.133860552049836E-4	0.0
6	6.341209379599853E-4	0.0	0.0	8.7191628969498E-4	7.926511724499817E-5
7	7.133860552049836E-4	0.0	0.0	8.7191628969498E-4	7.926511724499817E-5
8	7.133860552049836E-4	0.0	0.0	0.001347506993164969	7.926511724499817E-5
9	9.511814069399781E-4	0.0	0.0	0.0014267721104099672	7.926511724499817E-5
10	0.0015060372276549652	0.0	0.0	0.0021401581656149507	7.926511724499817E-5
11	0.00174383257938996	0.0	0.0	0.002219423282859949	7.926511724499817E-5
12	0.00174383257938996	0.0	0.0	0.002219423282859949	7.926511724499817E-5
13	0.0020608930483699524	0.0	0.0	0.0025364837518399414	7.926511724499817E-5
14	0.002219423282859949	0.0	0.0	0.0028535442208199343	2.3779535173499452E-4
15	0.002457218634594943	0.0	0.0	0.0030120744553099304	2.3779535173499452E-4
16	0.002695013986329938	0.0	0.0	0.0033291349242899233	3.170604689799927E-4
17	0.002695013986329938	0.0	0.0	0.0034084000415349216	3.170604689799927E-4
18	0.002774279103574936	0.0	0.0	0.00348766515877992	3.9632558622499086E-4
19	0.0030913395725549286	0.0	0.0	0.003646195393269916	4.7559070346998904E-4
20	0.004359581448474899	0.0	0.0	0.004518111682964896	4.7559070346998904E-4
21	0.00697533031755984	0.0	0.0	0.006658269848579847	5.548558207149872E-4
22	0.007133860552049835	0.0	0.0	0.0067375349658248445	5.548558207149872E-4
23	0.007213125669294834	0.0	0.0	0.00697533031755984	5.548558207149872E-4
24	0.007530186138274827	0.0	0.0	0.00737165590378483	5.548558207149872E-4
25	0.007530186138274827	0.0	0.0	0.007609451255519825	6.341209379599853E-4
26	0.009036223365929791	0.0	0.0	0.008798428014194797	6.341209379599853E-4
27	0.00911548848317479	0.0	0.0	0.009591079186644778	6.341209379599853E-4
28	0.009908139655624771	0.0	0.0	0.01212756293848472	6.341209379599853E-4
29	0.00998740477286977	0.0	0.0	0.014029925752364676	6.341209379599853E-4
30	0.01038373035909476	0.0	0.0	0.016804204855939614	6.341209379599853E-4
31	0.010700790828074753	0.0	0.0	0.024492921228704435	6.341209379599853E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGTAC	25	0.0054965434	29.6	31
ATACCGT	50	2.7023043E-4	22.2	6
CACCGTA	45	0.0038260121	20.555555	7
GGTATCA	1405	0.0	20.145908	1
TCTATAC	80	1.6168737E-5	18.5	3
TATACCC	245	0.0	17.367348	3
CCGATTT	225	0.0	17.266666	21
CTACTCG	285	0.0	16.228071	14
TACTCGG	290	0.0	15.9482765	15
GCACGTC	225	0.0	15.622222	28
TTGCACG	225	0.0	15.622222	26
GTATCAA	1830	0.0	15.467214	2
GACCGAT	240	0.0	15.416666	19
CTATACC	255	0.0	15.235294	2
TCGTACA	110	1.4520125E-5	15.136364	2
TAGTCCC	310	0.0	14.919354	5
ACGACCG	250	0.0	14.799999	17
TCGGACG	240	0.0	14.645833	13
ACCGATT	255	0.0	14.509804	20
CACGTCA	245	0.0	14.34694	29
>>END_MODULE