##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727577.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2310240
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.765560288108595	31.0	31.0	34.0	30.0	34.0
2	32.02760449130826	31.0	31.0	34.0	30.0	34.0
3	32.09358638063578	33.0	31.0	34.0	30.0	34.0
4	35.76219180691184	37.0	35.0	37.0	35.0	37.0
5	35.57507055544013	37.0	35.0	37.0	33.0	37.0
6	35.62294956368169	37.0	35.0	37.0	33.0	37.0
7	35.56032533416442	37.0	35.0	37.0	33.0	37.0
8	35.535764249601776	37.0	35.0	37.0	33.0	37.0
9	37.16229396080061	39.0	37.0	39.0	33.0	39.0
10	36.99313404667913	39.0	37.0	39.0	33.0	39.0
11	37.081071663550105	39.0	37.0	39.0	33.0	39.0
12	36.88702688898123	39.0	37.0	39.0	33.0	39.0
13	36.902600595609115	39.0	37.0	39.0	33.0	39.0
14	38.01426864741325	40.0	37.0	41.0	33.0	41.0
15	37.96404269686266	40.0	37.0	41.0	33.0	41.0
16	37.89413567421567	40.0	37.0	41.0	33.0	41.0
17	37.790200152365124	40.0	37.0	41.0	32.0	41.0
18	37.682301838769995	39.0	37.0	41.0	32.0	41.0
19	37.61967414640903	39.0	37.0	41.0	32.0	41.0
20	37.493528378004015	39.0	36.0	41.0	32.0	41.0
21	37.36098327446499	39.0	36.0	41.0	31.0	41.0
22	37.24234624974029	39.0	36.0	40.0	31.0	41.0
23	37.56885301959969	39.0	37.0	41.0	32.0	41.0
24	37.665335203268924	39.0	37.0	41.0	32.0	41.0
25	37.69813049726435	40.0	37.0	41.0	32.0	41.0
26	37.5618113269617	40.0	37.0	41.0	32.0	41.0
27	37.452385466445044	40.0	37.0	41.0	32.0	41.0
28	37.35273564651292	40.0	37.0	41.0	31.0	41.0
29	37.265168121061016	39.0	36.0	41.0	31.0	41.0
30	37.17896322460004	39.0	36.0	41.0	31.0	41.0
31	37.06857642496018	39.0	36.0	41.0	31.0	41.0
32	36.95363641872706	39.0	36.0	41.0	30.0	41.0
33	36.84390972366507	39.0	36.0	41.0	30.0	41.0
34	36.719281979361455	39.0	36.0	40.0	30.0	41.0
35	36.61738131103262	39.0	35.0	40.0	30.0	41.0
36	36.52604274880532	39.0	35.0	40.0	30.0	41.0
37	36.42700628506129	39.0	35.0	40.0	30.0	41.0
38	36.290972799362834	39.0	35.0	40.0	29.0	41.0
39	36.17780576909758	39.0	35.0	40.0	29.0	41.0
40	36.04904858369693	38.0	35.0	40.0	28.0	41.0
41	35.94294315742087	38.0	35.0	40.0	28.0	41.0
42	35.81208056305838	38.0	35.0	40.0	27.0	41.0
43	35.65974227785858	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	6.0
13	9.0
14	15.0
15	43.0
16	52.0
17	146.0
18	260.0
19	514.0
20	909.0
21	1673.0
22	3000.0
23	4863.0
24	7260.0
25	10724.0
26	15248.0
27	21156.0
28	29107.0
29	38510.0
30	48540.0
31	62653.0
32	77791.0
33	97791.0
34	124743.0
35	161745.0
36	219862.0
37	323168.0
38	546629.0
39	513822.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.58471847080823	20.83783503012674	13.536861971050627	26.040584528014403
2	21.30687720756285	22.662364083385274	31.69480227162546	24.335956437426415
3	20.934664796731077	23.669488884271765	29.8739957753307	25.521850543666456
4	17.197520603919937	17.088484313318098	32.73984521088718	32.974149871874786
5	15.33689140522197	36.122783780040166	33.578935521850546	14.96138929288732
6	35.321914606274674	35.57180206385483	14.212679202160814	14.893604127709676
7	29.357252926102916	30.48626982478011	20.42389535286377	19.732581896253205
8	27.417541034697695	33.959415471985594	18.984521088718058	19.638522404598657
9	26.804920700879563	14.105634046679134	18.83760994528707	40.25183530715423
10	16.65930812383129	27.702836068979845	32.84598136990096	22.791874437287902
11	36.6941962739802	22.012951035390262	20.650495186647273	20.64235750398227
12	22.044809197312834	25.543926172172586	28.705978599626015	23.705286030888566
13	31.020673176812796	20.53531234850059	23.47838319828243	24.96563127640418
14	22.675869173765495	21.16520361520881	24.792489092042384	31.366438118983307
15	25.32957614793268	28.387310409308125	21.654806427037883	24.628307015721308
16	24.8758137682665	26.375831082484936	23.905957822563888	24.842397326684672
17	24.339852136574557	26.497853036913916	24.688387353694853	24.473907472816677
18	24.72582935106309	25.423202784126325	25.452896668744373	24.39807119606621
19	25.50154962255004	25.259193849989614	25.518733984347946	23.720522543112406
20	24.942213795969252	25.284342752268163	25.621796869589307	24.15164658217328
21	24.861789251333192	25.320226469977147	25.598465960246553	24.219518318443107
22	25.090077221414226	25.244130479950133	25.199632938569156	24.466159360066484
23	24.89390712653231	25.256553431678093	25.17517660502805	24.67436283676155
24	24.580822771660088	25.613529330286035	25.219024863217676	24.586623034836208
25	24.89975067525452	25.357711752891475	25.377969388461807	24.3645681833922
26	24.622030611538197	25.500467483897776	25.428137336380637	24.449364568183395
27	24.837765773252997	25.756544774568873	25.226772975967865	24.17891647621026
28	24.658303899161993	25.31715319620472	25.478954567490824	24.545588337142462
29	24.551302029226402	25.532455502458618	25.378315672830524	24.537926795484452
30	24.526888981231387	25.66949754138098	25.214393309785997	24.58922016760163
31	24.796125077913985	24.941564512777894	25.581541311725186	24.680769097582935
32	24.390929080961286	25.49890920423852	25.297111988364847	24.81304972643535
33	24.461441235542626	25.201321074866676	25.559508968765147	24.777728720825543
34	24.83144608352379	25.071637578779693	25.622532723872844	24.474383613823672
35	24.912866195719925	25.559898538679963	25.340224392270933	24.18701087332918
36	24.668605859131517	25.422207216566246	25.489126670822078	24.42006025348016
37	24.869580649629476	25.26304626359166	25.39008934136713	24.477283745411732
38	24.486416995636816	25.176128887042037	25.829437634185194	24.508016483135954
39	24.65609633631138	25.278152919177227	25.56565551630999	24.5000952282014
40	24.694793614516243	24.994459450100422	25.979335480296417	24.33141145508692
41	24.607400096959623	24.904252372047925	25.933972227993628	24.554375302998825
42	24.55606343929635	24.993204169263798	25.66737654962255	24.7833558418173
43	24.54913775192188	24.586363321559666	25.91743714938708	24.94706177713138
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	107.0
1	133.5
2	160.0
3	192.0
4	224.0
5	224.0
6	372.0
7	520.0
8	571.5
9	623.0
10	919.5
11	1216.0
12	1216.0
13	2791.5
14	4367.0
15	7015.5
16	9664.0
17	8951.0
18	8238.0
19	8238.0
20	10532.5
21	12827.0
22	12831.0
23	12835.0
24	16253.0
25	19671.0
26	19671.0
27	24855.0
28	30039.0
29	35336.0
30	40633.0
31	48235.5
32	55838.0
33	55838.0
34	64342.5
35	72847.0
36	81849.5
37	90852.0
38	102228.0
39	113604.0
40	113604.0
41	123935.0
42	134266.0
43	145128.5
44	155991.0
45	165244.5
46	174498.0
47	174498.0
48	183626.5
49	192755.0
50	197009.5
51	201264.0
52	197936.5
53	194609.0
54	194609.0
55	188339.0
56	182069.0
57	171863.0
58	161657.0
59	148279.0
60	134901.0
61	134901.0
62	118993.0
63	103085.0
64	87864.5
65	72644.0
66	60294.0
67	47944.0
68	47944.0
69	39679.0
70	31414.0
71	25403.0
72	19392.0
73	15482.5
74	11573.0
75	11573.0
76	9312.0
77	7051.0
78	5681.0
79	4311.0
80	3445.0
81	2579.0
82	2579.0
83	2108.0
84	1637.0
85	1271.5
86	906.0
87	838.0
88	770.0
89	770.0
90	577.0
91	384.0
92	260.5
93	137.0
94	122.5
95	108.0
96	108.0
97	63.0
98	18.0
99	15.0
100	12.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2310240.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.17551246840702
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.81987893788352	50.665485365964535
2	9.465990399298294	11.581736273961878
3	2.9368576609373704	5.389913173638324
4	1.3754430097809738	3.36573723977757
5	0.8191125301074335	2.505481439930786
6	0.5068778582462243	1.8605107642260803
7	0.35666562187568696	1.5273441538675765
8	0.2576833088070387	1.26111267766603
9	0.19791087176188898	1.089656910279247
>10	1.14943633863696	12.88920217479167
>50	0.07799685263328147	3.2906929541555474
>100	0.03466351873051812	3.4462743017925006
>500	0.0010593509291708465	0.41921058531607364
>1k	4.237403716678641E-4	0.7076419846321574
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	4920	0.2129648867650114	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	4367	0.18902797977699287	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2976	0.12881778516517764	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	4.3285546090449475E-5	1.2985663827134842E-4
2	1.2985663827134842E-4	0.0	0.0	1.731421843617979E-4	1.2985663827134842E-4
3	3.0299882263314635E-4	0.0	0.0	2.1642773045224737E-4	1.2985663827134842E-4
4	3.895699148140453E-4	0.0	0.0	2.1642773045224737E-4	1.2985663827134842E-4
5	0.001168709744442136	0.0	0.0	3.462843687235958E-4	1.2985663827134842E-4
6	0.0012552808366230348	0.0	0.0	6.492831913567421E-4	1.2985663827134842E-4
7	0.0013851374748943832	0.0	0.0	6.492831913567421E-4	1.2985663827134842E-4
8	0.0017747073897084287	0.0	0.0	7.791398296280906E-4	1.2985663827134842E-4
9	0.001817992935798878	0.0	0.0	7.791398296280906E-4	2.1642773045224737E-4
10	0.0032031304106932613	0.0	0.0	0.0010821386522612368	2.1642773045224737E-4
11	0.003462843687235958	0.0	0.0	0.0011254241983516865	2.1642773045224737E-4
12	0.0035927003255073068	0.0	0.0	0.0012985663827134843	2.1642773045224737E-4
13	0.003938984694230902	0.0	0.0	0.0016015652053466306	2.1642773045224737E-4
14	0.004285269062954498	0.0	0.0	0.001817992935798878	2.1642773045224737E-4
15	0.004674838977768544	0.0	0.0	0.001904564027979777	2.1642773045224737E-4
16	0.005021123346492139	0.0	0.0	0.001991135120160676	2.5971327654269685E-4
17	0.005107694438673038	0.0	0.0	0.0020344206662511253	2.5971327654269685E-4
18	0.005150979984763488	0.0	0.0	0.0021642773045224737	2.5971327654269685E-4
19	0.005540549899577533	0.0	0.0	0.0023807050349747214	2.5971327654269685E-4
20	0.006925687374471916	0.0	0.0	0.002813560495879216	2.5971327654269685E-4
21	0.009652676778170234	0.0	0.0	0.003982270240321352	3.0299882263314635E-4
22	0.009825818962532032	0.0	0.0	0.004068841332502251	3.0299882263314635E-4
23	0.00999896114689383	0.0	0.0	0.004415125701225846	3.0299882263314635E-4
24	0.010301959969526975	0.0	0.0	0.004674838977768544	3.0299882263314635E-4
25	0.01073481543043147	0.0	0.0	0.004847981162130342	3.0299882263314635E-4
26	0.011903525174873607	0.0	0.0	0.0057136920839393306	3.0299882263314635E-4
27	0.012076667359235405	0.0	0.0	0.00744511392755731	3.462843687235958E-4
28	0.012596093912320798	0.0	0.0	0.01164381189833091	3.462843687235958E-4
29	0.012639379458411247	0.0	0.0	0.01908892582588822	3.462843687235958E-4
30	0.012942378281044394	0.0	0.0	0.0289580303345107	3.462843687235958E-4
31	0.013375233741948888	0.0	0.0	0.044281113650529814	3.462843687235958E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2120	0.0	22.426888	1
TAAGGCG	465	0.0	16.311827	29
GCGAATC	425	0.0	16.105883	33
GGCGAAT	425	0.0	16.105883	32
TATACAC	610	0.0	15.467213	3
GGGTATA	530	0.0	15.009434	1
CGAATCT	470	0.0	14.957446	34
GTATCAA	3280	0.0	14.382621	2
GAGACTA	530	0.0	13.962264	24
ATCTCCG	575	0.0	13.834783	10
CTTATAC	620	0.0	13.725806	1
AATCTCG	475	0.0	13.631578	36
CGAGACT	560	0.0	13.544642	23
ACGAGAC	565	0.0	13.424778	22
AGGCGAA	545	0.0	13.238532	31
TACACAT	785	0.0	13.197453	5
ATACCGA	85	0.009408487	13.058824	6
CACGAGA	585	0.0	12.965813	21
GCCCACG	645	0.0	12.906977	18
CGAGCCC	625	0.0	12.728001	15
>>END_MODULE