FastQCFastQC Report
Thu 9 Feb 2017
SRR2727576.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727576.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7777554
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1915742.463165154494588No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1637272.105121995938569No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1002431.2888756542223945No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT736050.946377228624835No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT268440.34514707323150695No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT223780.28772542112854504No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTG166530.21411616042781573No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT153440.19728567619074067No Hit
TATCAACGCAGAGTACGGGTCATCTCCCCCAGATCTTGACTAT123530.1588288554473553No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAG115930.14905714572987858No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCG111360.1431812623866064No Hit
ATACTAAAGTTTGATTAGCCAATAGATTCCTGTGGGGAAAAAA110870.14255124426008486No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT108460.13945258367862184No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107450.1381539748872203No Hit
GGTATCAACGCAGAGTACGGGTCATCTCCCCCAGATCTTGACT99330.12771367450486362No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95040.12219780151960372No Hit
GTATCAACGCAGAGTACGGGTCATCTCCCCCAGATCTTGACTA92770.11927914611714685No Hit
GATCTTGACTATATACTAAAGTTTGATTAGCCAATAGATTCCT92180.11852055286276379No Hit
GAGTACGGGTCATCTCCCCCAGATCTTGACTATATACTAAAGT91640.11782624717231151No Hit
CCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTT84960.10923742863116091No Hit
GAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTG84290.10837597527448861No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGACTA15750.019.73333424
CACGAGA16700.019.2754521
ACGAGAC17050.019.09677522
CGAGACT17700.018.2909623
CCCACGA18450.017.54742619
GCCCACG18500.017.518
GACTAGG18300.017.08469826
CCACGAG19850.016.77581820
ATCTCCG19450.016.45501310
TAGGCAT19950.015.94987529
CCGAGCC21150.015.91962214
CTAGGCA20400.015.77941128
ACTAGGC19750.015.7367127
TCCGAGC20700.015.55072513
GGCATGA20400.015.50735331
TACACAT22900.015.430135
ATACACA24050.015.0769234
AGGCATG21300.015.02582230
ACGTTTA2600.014.942306526
CTCCGAG22350.014.89932912
Produced by FastQC (version 0.11.5)