##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727576.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7777554
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.860154747880888	31.0	31.0	34.0	30.0	34.0
2	32.087172393788585	33.0	31.0	34.0	30.0	34.0
3	32.082013702508526	33.0	31.0	34.0	30.0	34.0
4	35.7294565875081	37.0	35.0	37.0	35.0	37.0
5	35.66409143543073	37.0	35.0	37.0	33.0	37.0
6	35.73546708901025	37.0	35.0	37.0	35.0	37.0
7	35.634465283044	37.0	35.0	37.0	33.0	37.0
8	35.65388038450135	37.0	35.0	37.0	33.0	37.0
9	37.24147951913931	39.0	37.0	39.0	34.0	39.0
10	37.14610300359213	39.0	37.0	39.0	33.0	39.0
11	37.21388613952407	39.0	37.0	39.0	34.0	39.0
12	37.0926469941578	39.0	37.0	39.0	33.0	39.0
13	37.08356328994952	39.0	37.0	39.0	33.0	39.0
14	38.07087639635803	40.0	37.0	41.0	33.0	41.0
15	38.080791338767945	40.0	37.0	41.0	33.0	41.0
16	37.954970290145205	40.0	37.0	41.0	33.0	41.0
17	38.02811166595565	40.0	37.0	41.0	33.0	41.0
18	38.02797511916986	40.0	37.0	41.0	33.0	41.0
19	38.05011948486632	40.0	37.0	41.0	33.0	41.0
20	37.97153153806454	40.0	37.0	41.0	33.0	41.0
21	37.84928654947301	40.0	37.0	41.0	32.0	41.0
22	37.709430882768544	40.0	37.0	41.0	32.0	41.0
23	37.87001273150916	40.0	37.0	41.0	33.0	41.0
24	37.85707691647014	40.0	37.0	41.0	32.0	41.0
25	37.8455928431998	40.0	37.0	41.0	32.0	41.0
26	37.6014838341206	40.0	37.0	41.0	32.0	41.0
27	37.52783741520792	40.0	36.0	41.0	32.0	41.0
28	37.34938799524889	40.0	36.0	41.0	32.0	41.0
29	37.20698795533917	39.0	36.0	41.0	31.0	41.0
30	36.99979672272285	39.0	35.0	41.0	31.0	41.0
31	36.75634897552624	39.0	35.0	41.0	31.0	41.0
32	36.52302677679898	38.0	35.0	41.0	30.0	41.0
33	36.307469289187836	38.0	35.0	40.0	30.0	41.0
34	36.13739692453437	38.0	35.0	40.0	30.0	41.0
35	35.91913421623302	38.0	35.0	40.0	30.0	41.0
36	35.74791791866697	38.0	35.0	40.0	29.0	41.0
37	35.5471968436349	38.0	35.0	40.0	27.0	41.0
38	35.30007403355862	38.0	35.0	40.0	26.0	41.0
39	35.03679858731935	38.0	34.0	40.0	25.0	41.0
40	34.73165612222043	38.0	34.0	40.0	24.0	41.0
41	34.42233547976652	37.0	33.0	40.0	23.0	41.0
42	34.0680307716282	37.0	33.0	40.0	21.0	41.0
43	33.6889018064034	37.0	33.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	5.0
12	18.0
13	38.0
14	51.0
15	120.0
16	262.0
17	567.0
18	1053.0
19	2077.0
20	4056.0
21	7331.0
22	12180.0
23	19203.0
24	29941.0
25	43826.0
26	61944.0
27	84675.0
28	111720.0
29	142273.0
30	177470.0
31	218344.0
32	271630.0
33	344485.0
34	442738.0
35	560742.0
36	770317.0
37	1272010.0
38	1516797.0
39	1681679.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.92136653760295	19.905975580497415	14.435566760449364	21.737091121450266
2	20.65715005000287	25.532667468461163	30.59929895697285	23.210883524563123
3	21.253134340179443	24.554069312794226	31.50225636491884	22.690539982107484
4	15.059760433678763	17.721342725489272	31.334362962957247	35.884533877874716
5	14.818412061169875	37.73055127614672	31.494516142221578	15.956520520461831
6	32.768888522021186	35.20154279867423	12.59714815223398	19.432420527070594
7	28.028220697664075	31.680281486955923	19.99919254819703	20.29230526718297
8	30.07748965805959	33.424840251832386	17.00047341362079	19.497196676487235
9	26.71013277439154	13.59796408999539	19.645752379218454	40.046150756394624
10	19.64154797253738	29.513777724976258	28.70586305154551	22.138811250940847
11	33.744516592234525	23.422788192791717	18.902896720485643	23.929798494488114
12	22.6223694493153	29.79502295966058	27.717171748341446	19.865435842682675
13	34.395003879111606	19.268410093970417	24.206505027158926	22.13008099975905
14	21.64458131695389	23.32487823292516	29.1688620869749	25.861678363146044
15	26.52695178972721	27.720347553999623	25.00395368518174	20.748746971091425
16	21.626516511489342	24.98531029164182	28.475147842110772	24.913025354758066
17	23.080508344911525	26.400665299141608	27.913608828688304	22.60521752725857
18	23.894697484581915	22.061061871122977	29.531263942365428	24.51297670192968
19	24.36980829705586	23.699636158103175	31.678172854858992	20.252382689981964
20	23.81779670060793	23.492295906913665	31.446994774963954	21.242912617514452
21	22.183002522386857	25.634653259880935	31.43303151607819	20.749312701654016
22	22.003241636123644	25.368669892873775	30.796018902601	21.832069568401582
23	21.376797898156667	24.91569971741758	31.40635217704692	22.30115020737882
24	21.10502865039574	26.005888226555545	31.622075526573006	21.267007596475704
25	22.147322924405284	24.696273404209087	31.16533809987047	21.991065571515158
26	21.098497033900376	26.328521794898496	31.642081816468266	20.930899354732862
27	22.365489201360734	24.865683992679447	32.12764578683735	20.641181019122463
28	20.98105651211165	26.165938545717587	31.440977458980036	21.412027483190734
29	21.249328516394744	26.539217856925195	31.31058942181565	20.90086420486441
30	20.714700277233693	26.484406279917827	31.48127290405184	21.319620538796645
31	20.87926358338367	26.361411312605483	31.213309480075612	21.54601562393524
32	20.43130783791408	26.69039649226479	30.496413139658046	22.381882530163082
33	20.316323101067507	27.201019755054094	31.1937274881023	21.288929655776094
34	21.596725654363826	27.41648595432446	30.34115867276524	20.645629718546473
35	20.93793241422689	28.171684825331972	30.667958075250905	20.22242468519023
36	20.702357064958985	27.914971725043632	30.350313221868987	21.0323579881284
37	20.901069925068988	27.40079978872535	29.829853961798275	21.86827632440739
38	20.56717317552536	27.786628032412246	30.504307652508743	21.141891139553643
39	20.824760586683166	28.376890729398983	29.58637124216688	21.211977441750967
40	21.048095583778654	28.806447374071592	29.700918823578725	20.444538218571033
41	20.964650325796516	28.284560930081614	29.497500113788988	21.25328863033288
42	20.23010062032356	28.386315286271234	29.501987385751356	21.88159670765385
43	20.867948972131856	27.983077970271886	29.45955759355705	21.689415464039207
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1636.0
1	2796.0
2	3956.0
3	7280.0
4	10604.0
5	10604.0
6	15678.5
7	20753.0
8	20196.0
9	19639.0
10	29831.5
11	40024.0
12	40024.0
13	98513.5
14	157003.0
15	248176.0
16	339349.0
17	279787.0
18	220225.0
19	220225.0
20	250952.0
21	281679.0
22	194474.0
23	107269.0
24	112660.0
25	118051.0
26	118051.0
27	129366.5
28	140682.0
29	167169.5
30	193657.0
31	208613.0
32	223569.0
33	223569.0
34	226806.5
35	230044.0
36	242886.0
37	255728.0
38	262324.0
39	268920.0
40	268920.0
41	298166.0
42	327412.0
43	355804.5
44	384197.0
45	398410.5
46	412624.0
47	412624.0
48	463623.0
49	514622.0
50	531110.5
51	547599.0
52	568195.5
53	588792.0
54	588792.0
55	559294.5
56	529797.0
57	459583.5
58	389370.0
59	359594.5
60	329819.0
61	329819.0
62	302289.0
63	274759.0
64	241640.0
65	208521.0
66	189810.5
67	171100.0
68	171100.0
69	162963.5
70	154827.0
71	132594.0
72	110361.0
73	87385.5
74	64410.0
75	64410.0
76	55903.0
77	47396.0
78	40095.5
79	32795.0
80	26973.5
81	21152.0
82	21152.0
83	17370.5
84	13589.0
85	11698.0
86	9807.0
87	7971.5
88	6136.0
89	6136.0
90	4699.0
91	3262.0
92	2356.5
93	1451.0
94	1062.0
95	673.0
96	673.0
97	437.5
98	202.0
99	147.5
100	93.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	7777554.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.910000801278475
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.69447481946347	23.19680749737597
2	13.29343150354637	8.483868198598104
3	4.9859028790880435	4.773004946003884
4	2.454832505121738	3.1333482882175643
5	1.409730359913999	2.2492248457221153
6	0.9413682900500345	1.802343773387685
7	0.6543917066052907	1.461714791748737
8	0.4917200572099419	1.2552629935659156
9	0.3586619334642073	1.030041232580785
>10	2.2916807387720572	13.92771410663047
>50	0.22485712466462945	4.97676616480213
>100	0.16820000505446114	10.655980263884942
>500	0.016278442363158226	3.562465332941003
>1k	0.012660930347503315	8.042126376568506
>5k	0.001245996319799689	2.7518488063513216
>10k+	5.627080153934079E-4	8.697482381620777
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	191574	2.463165154494588	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	163727	2.105121995938569	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	100243	1.2888756542223945	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	73605	0.946377228624835	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	26844	0.34514707323150695	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	22378	0.28772542112854504	No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTG	16653	0.21411616042781573	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15344	0.19728567619074067	No Hit
TATCAACGCAGAGTACGGGTCATCTCCCCCAGATCTTGACTAT	12353	0.1588288554473553	No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAG	11593	0.14905714572987858	No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCG	11136	0.1431812623866064	No Hit
ATACTAAAGTTTGATTAGCCAATAGATTCCTGTGGGGAAAAAA	11087	0.14255124426008486	No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT	10846	0.13945258367862184	No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10745	0.1381539748872203	No Hit
GGTATCAACGCAGAGTACGGGTCATCTCCCCCAGATCTTGACT	9933	0.12771367450486362	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9504	0.12219780151960372	No Hit
GTATCAACGCAGAGTACGGGTCATCTCCCCCAGATCTTGACTA	9277	0.11927914611714685	No Hit
GATCTTGACTATATACTAAAGTTTGATTAGCCAATAGATTCCT	9218	0.11852055286276379	No Hit
GAGTACGGGTCATCTCCCCCAGATCTTGACTATATACTAAAGT	9164	0.11782624717231151	No Hit
CCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTT	8496	0.10923742863116091	No Hit
GAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTG	8429	0.10837597527448861	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.6714766621999668E-4	0.0	0.0	1.6714766621999668E-4	0.0
2	0.00282865281295379	0.0	0.0	2.3143523015076463E-4	0.0
3	0.0041786916554999165	0.0	0.0	2.82865281295379E-4	0.0
4	0.004538702013512218	0.0	0.0	6.557331520938331E-4	0.0
5	0.014168979090341257	0.0	0.0	0.001941484430709192	0.0
6	0.020752025636851894	0.0	0.0	0.0026486476339476397	0.0
7	0.023644966013736453	0.0	0.0	0.0029958004791737864	0.0
8	0.03040801773925324	0.0	0.0	0.003934398912562999	0.0
9	0.03225949958045936	0.0	0.0	0.004217264193858377	0.0
10	0.04735421959140367	0.0	0.0	0.00633875380357372	0.0
11	0.05089003560759591	0.0	0.0	0.006955914417309092	1.285751278615359E-5
12	0.05347439567761278	0.0	1.285751278615359E-5	0.0074573574159690825	1.285751278615359E-5
13	0.05747308215410655	0.0	1.285751278615359E-5	0.008190235644779838	1.285751278615359E-5
14	0.06531616495366024	0.0	1.285751278615359E-5	0.009694564640759807	1.285751278615359E-5
15	0.06977772189045553	0.0	1.285751278615359E-5	0.010504587946287483	1.285751278615359E-5
16	0.07434213892954006	0.0	1.285751278615359E-5	0.011340326277387467	1.285751278615359E-5
17	0.07589789797666464	0.0	1.285751278615359E-5	0.011623191558682846	1.285751278615359E-5
18	0.07763366220279538	0.0	2.571502557230718E-5	0.012073204506198221	1.285751278615359E-5
19	0.08087375542490609	0.0	2.571502557230718E-5	0.012806082735008975	1.285751278615359E-5
20	0.09968429663104879	0.0	3.857253835846077E-5	0.01693334433936428	1.285751278615359E-5
21	0.14257695928565717	0.0	3.857253835846077E-5	0.025162152522502576	1.285751278615359E-5
22	0.14528989448353558	0.0	5.143005114461436E-5	0.025753598110665642	1.285751278615359E-5
23	0.14847855765450166	0.0	5.143005114461436E-5	0.02657647892897947	1.285751278615359E-5
24	0.15398157312697539	0.0	6.428756393076796E-5	0.028337958180682514	1.285751278615359E-5
25	0.1574145290408784	0.0	6.428756393076796E-5	0.02964942448487018	1.285751278615359E-5
26	0.17729224380827185	0.0	6.428756393076796E-5	0.035075294880626995	1.285751278615359E-5
27	0.18274382922960097	0.0	6.428756393076796E-5	0.040424020199666885	1.285751278615359E-5
28	0.19113978507895926	0.0	6.428756393076796E-5	0.056984496668232715	1.285751278615359E-5
29	0.19230981874249925	0.0	6.428756393076796E-5	0.09996716191234416	1.285751278615359E-5
30	0.19458559850564844	0.0	6.428756393076796E-5	0.1496357338052555	1.285751278615359E-5
31	0.1998057486968268	0.0	6.428756393076796E-5	0.2438813025277613	1.285751278615359E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAGACTA	1575	0.0	19.733334	24
CACGAGA	1670	0.0	19.27545	21
ACGAGAC	1705	0.0	19.096775	22
CGAGACT	1770	0.0	18.29096	23
CCCACGA	1845	0.0	17.547426	19
GCCCACG	1850	0.0	17.5	18
GACTAGG	1830	0.0	17.084698	26
CCACGAG	1985	0.0	16.775818	20
ATCTCCG	1945	0.0	16.455013	10
TAGGCAT	1995	0.0	15.949875	29
CCGAGCC	2115	0.0	15.919622	14
CTAGGCA	2040	0.0	15.779411	28
ACTAGGC	1975	0.0	15.73671	27
TCCGAGC	2070	0.0	15.550725	13
GGCATGA	2040	0.0	15.507353	31
TACACAT	2290	0.0	15.43013	5
ATACACA	2405	0.0	15.076923	4
AGGCATG	2130	0.0	15.025822	30
ACGTTTA	260	0.0	14.9423065	26
CTCCGAG	2235	0.0	14.899329	12
>>END_MODULE