FastQCFastQC Report
Thu 9 Feb 2017
SRR2727575.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727575.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9317332
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT182750.19613983917284475No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT180260.19346740032447057No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT122300.1312607514683388No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA114350.015.0297341
TAATCCC33250.013.4090225
TTAATCC35450.012.4724974
TTTAATC38700.011.9031013
CGTATAC2052.8177055E-711.7317073
CCTTTAA44950.011.4416011
GTATCAA155900.011.000322
TAATACC9250.010.84
CTTTAAT47900.010.4279752
GGCGGGT8350.010.41317433
TATACTG10300.010.23786455
CGCAATC4550.010.16483522
GCACTCG23200.010.04741513
GTATAGA9850.09.9543151
GGGTAAG30500.09.8868861
GCGGGTT9500.09.73684234
TCTACAC19800.09.7171723
CTAACAC12600.09.6904753
GTATTAG10700.09.6822421
GTCTTAC9200.09.6521741
Produced by FastQC (version 0.11.5)