##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727575.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9317332
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.30656672961745	33.0	31.0	34.0	31.0	34.0
2	32.539948345728156	34.0	31.0	34.0	31.0	34.0
3	32.61601218031085	34.0	31.0	34.0	31.0	34.0
4	36.14315578751514	37.0	35.0	37.0	35.0	37.0
5	36.04460128714958	37.0	35.0	37.0	35.0	37.0
6	36.03797889782182	37.0	35.0	37.0	35.0	37.0
7	35.97422137581874	37.0	35.0	37.0	35.0	37.0
8	35.963781048051096	37.0	35.0	37.0	35.0	37.0
9	37.64777685285873	39.0	37.0	39.0	35.0	39.0
10	37.595269439792425	39.0	37.0	39.0	35.0	39.0
11	37.61669424251492	39.0	37.0	39.0	35.0	39.0
12	37.494389702975056	39.0	37.0	39.0	35.0	39.0
13	37.48264835899376	39.0	37.0	39.0	35.0	39.0
14	38.763403622410365	40.0	38.0	41.0	35.0	41.0
15	38.736338149161156	40.0	38.0	41.0	34.0	41.0
16	38.6615676032581	40.0	38.0	41.0	34.0	41.0
17	38.57967302227719	40.0	38.0	41.0	34.0	41.0
18	38.47298711691287	40.0	38.0	41.0	34.0	41.0
19	38.43087634958162	40.0	38.0	41.0	34.0	41.0
20	38.30901270878831	40.0	38.0	41.0	33.0	41.0
21	38.20780959613761	40.0	38.0	41.0	33.0	41.0
22	38.094162577871	40.0	38.0	41.0	33.0	41.0
23	38.268000861190735	40.0	38.0	41.0	33.0	41.0
24	38.25159187200799	40.0	38.0	41.0	33.0	41.0
25	38.23904021022327	40.0	38.0	41.0	33.0	41.0
26	38.10665113146124	40.0	38.0	41.0	33.0	41.0
27	38.15312065728687	40.0	38.0	41.0	33.0	41.0
28	38.03975365480161	40.0	38.0	41.0	33.0	41.0
29	38.016861157249735	40.0	38.0	41.0	33.0	41.0
30	37.956149356918914	40.0	38.0	41.0	33.0	41.0
31	37.86843154242008	40.0	37.0	41.0	33.0	41.0
32	37.76030434463428	40.0	37.0	41.0	32.0	41.0
33	37.66275238448088	40.0	37.0	41.0	32.0	41.0
34	37.57058136384965	40.0	37.0	41.0	32.0	41.0
35	37.459060920014444	40.0	37.0	41.0	31.0	41.0
36	37.36407503778979	40.0	37.0	41.0	31.0	41.0
37	37.26855016006728	40.0	37.0	41.0	31.0	41.0
38	37.14089108341315	40.0	36.0	41.0	31.0	41.0
39	37.02665237215975	40.0	36.0	41.0	30.0	41.0
40	36.91552624721326	40.0	36.0	41.0	30.0	41.0
41	36.796392465139164	40.0	36.0	41.0	30.0	41.0
42	36.67393884858885	39.0	35.0	41.0	30.0	41.0
43	36.54131408003922	39.0	35.0	41.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	0.0
12	11.0
13	16.0
14	33.0
15	58.0
16	139.0
17	322.0
18	612.0
19	1292.0
20	2530.0
21	4866.0
22	8444.0
23	13582.0
24	21178.0
25	31202.0
26	44121.0
27	61585.0
28	83721.0
29	111831.0
30	145432.0
31	187093.0
32	238207.0
33	302774.0
34	394810.0
35	516719.0
36	705742.0
37	1052645.0
38	1842578.0
39	3545787.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.273653874306504	17.749029443192534	11.757239089473252	27.220077593027703
2	20.366066165722117	20.467135871084125	32.71944157404717	26.44735638914659
3	20.45038214802263	21.465286414608816	29.234377394730593	28.849954042637954
4	15.96679178116654	15.11230897428577	32.276653874735814	36.64424536981187
5	16.004055667437846	34.829412539984624	33.08639211310705	16.080139679470477
6	36.87354920915129	33.311123828151665	14.078590308899585	15.736736653797461
7	30.783554777268858	29.247299548840804	19.652385468286415	20.316760205603924
8	28.153273920044924	32.90806853292337	18.555086370218426	20.383571176813277
9	27.225508332213554	13.876590423095367	18.236314859232237	40.66158638545884
10	17.674780720489515	26.284251757906663	31.77892555508379	24.26204196652003
11	37.18502249356361	20.979009871066097	20.073257022503867	21.762710612866428
12	22.497835217205957	24.33575405491615	28.242795255122388	24.923615472755507
13	31.851972216939355	19.22107101045664	22.760871889077258	26.166084883526743
14	23.209873813662536	19.995037205929766	23.859287186503604	32.93580179390409
15	26.278531236195086	26.893460488474595	21.12254881547636	25.70545945985396
16	25.851520585506666	25.21707930982818	23.146508034703498	25.784892069961657
17	25.25303380839064	25.196912592574783	24.08385791125614	25.46619568777843
18	25.5514024830284	24.441599805609588	24.67693541455859	25.33006229680342
19	26.394669632894914	24.211394420634576	24.676087532353684	24.71784841411683
20	25.968979102601473	24.11989827130771	24.756325093921735	25.15479753216908
21	25.77529704855424	24.224874674423965	24.729139199934057	25.27068907708773
22	26.024585149482704	24.110131526922085	24.195102203077017	25.670181120518194
23	25.720227635979914	24.14977806951604	24.294680065065837	25.83531422943821
24	25.509695264695946	24.311970422434236	24.40298360088489	25.775350711984935
25	25.864228085894116	23.98522452564747	24.375228874531892	25.77531851392652
26	25.504071337159605	24.38756073090451	24.534823917404683	25.573544014531198
27	25.966993555665933	24.359612816201032	24.416764369886142	25.25662925824689
28	25.565430103810833	24.43240189358928	24.417504925229668	25.584663077370216
29	25.499155766908384	24.619987781910098	24.50827125189915	25.372585199282373
30	25.708507542717165	24.462152899563954	24.39395741184279	25.435382145876094
31	25.867082980406835	23.946479528689114	24.458246201809704	25.728191289094344
32	25.409752491378434	24.373747763844843	24.36409907900674	25.852400665769988
33	25.392537262813004	24.074466810885347	24.583936689172393	25.94905923712926
34	25.906128492577057	24.107341028526193	24.640186697221907	25.34634378167484
35	25.758586256237297	24.499889024025332	24.39842220927622	25.34310251046115
36	25.48812256555847	24.32507503220879	24.61295787248968	25.573844529743063
37	25.76513319478151	24.235993737262984	24.45993123353338	25.53894183442213
38	25.464639448288416	24.292683785444158	24.731500390884428	25.511176375382995
39	25.361047561683968	24.482663062773764	24.696050328570454	25.460239046971818
40	25.80023981113907	24.10693318645295	24.80065108767188	25.29217591473611
41	25.412822039613914	24.274567011243136	24.79706637050177	25.51554457864118
42	25.408893876487387	24.172660156362358	24.652153642265834	25.76629232488442
43	25.566063332292977	23.864471073908284	24.605369863390077	25.96409573040866
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	199.0
1	413.5
2	628.0
3	1247.0
4	1866.0
5	1866.0
6	2452.5
7	3039.0
8	2750.5
9	2462.0
10	3565.0
11	4668.0
12	4668.0
13	10743.5
14	16819.0
15	24867.0
16	32915.0
17	30118.5
18	27322.0
19	27322.0
20	34899.0
21	42476.0
22	39101.0
23	35726.0
24	44816.5
25	53907.0
26	53907.0
27	69023.5
28	84140.0
29	101378.5
30	118617.0
31	142369.0
32	166121.0
33	166121.0
34	195530.5
35	224940.0
36	260918.0
37	296896.0
38	339927.0
39	382958.0
40	382958.0
41	426996.5
42	471035.0
43	519387.0
44	567739.0
45	610892.0
46	654045.0
47	654045.0
48	703153.0
49	752261.0
50	782507.5
51	812754.0
52	820178.5
53	827603.0
54	827603.0
55	804854.0
56	782105.0
57	737360.0
58	692615.0
59	641965.5
60	591316.0
61	591316.0
62	531674.5
63	472033.0
64	409181.0
65	346329.0
66	300852.0
67	255375.0
68	255375.0
69	221250.5
70	187126.0
71	164267.5
72	141409.0
73	118316.0
74	95223.0
75	95223.0
76	80526.0
77	65829.0
78	54901.5
79	43974.0
80	35115.5
81	26257.0
82	26257.0
83	20726.5
84	15196.0
85	12968.0
86	10740.0
87	8288.5
88	5837.0
89	5837.0
90	4307.5
91	2778.0
92	2012.5
93	1247.0
94	920.5
95	594.0
96	594.0
97	370.0
98	146.0
99	106.5
100	67.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	9317332.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.48140819251888
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.94182127117793	34.83401385196415
2	12.431028912347667	11.556234582556717
3	4.579869508204405	6.386363522379589
4	2.207311179609491	4.1039572778935645
5	1.331314205547396	3.094067951027374
6	0.8571144189035929	2.390393110365091
7	0.5856684173599126	1.9055884940940828
8	0.4320651958119173	1.6066398985851462
9	0.35360636029334586	1.4792509415039319
>10	2.019451198691399	17.52045614873062
>50	0.16901911645575385	5.37435632839534
>100	0.08685320314828086	7.330056976982627
>500	0.0038374299281051603	1.2019243789189715
>1k	9.471752602186448E-4	0.616396644674341
>5k	2.3101815174868286E-5	0.07917163536040187
>10k+	6.930544552460485E-5	0.5211282565679727
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	18275	0.19613983917284475	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	18026	0.19346740032447057	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	12230	0.1312607514683388	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.293074455219584E-5	0.0	0.0	2.146537227609792E-5	3.2198058414146884E-5
2	1.8245566434683233E-4	0.0	0.0	4.293074455219584E-5	3.2198058414146884E-5
3	2.0392103662293025E-4	0.0	0.0	6.439611682829377E-5	3.2198058414146884E-5
4	2.253864088990282E-4	0.0	0.0	6.439611682829377E-5	3.2198058414146884E-5
5	0.001073268613804896	0.0	0.0	1.0732686138048961E-4	3.2198058414146884E-5
6	0.0015562394900170992	0.0	0.0	1.3952491979463648E-4	5.3663430690244805E-5
7	0.0017494278405019805	0.0	0.0	1.5025760593268545E-4	7.512880296634273E-5
8	0.0022216660305761347	0.0	0.0	2.253864088990282E-4	9.659417524244065E-5
9	0.002457785125613212	0.0	0.0	2.575844673131751E-4	1.3952491979463648E-4
10	0.003777905520593234	0.0	0.0	3.756440148317136E-4	1.3952491979463648E-4
11	0.004003291929492263	0.0	0.0	4.185747593839095E-4	1.3952491979463648E-4
12	0.004260876396805438	0.0	0.0	4.185747593839095E-4	1.5025760593268545E-4
13	0.004604322353223004	0.0	0.0	4.615055039361053E-4	1.7172297820878336E-4
14	0.005280481579920089	0.0	0.0	5.47366993040497E-4	2.68317153451224E-4
15	0.005699056339303998	0.0	0.0	5.580996791785459E-4	2.7904983958927296E-4
16	0.00608543304027376	0.0	0.0	5.902977375926928E-4	3.005152118653709E-4
17	0.0062034925877923	0.0	0.0	5.902977375926928E-4	3.005152118653709E-4
18	0.006289354076896691	0.0	0.0	6.117631098687908E-4	3.6491132869366467E-4
19	0.006654265405590356	0.0	0.0	6.439611682829377E-4	3.756440148317136E-4
20	0.008618346968853316	0.0	0.0	8.2641683262977E-4	3.756440148317136E-4
21	0.01322266932207632	0.0	0.0	0.0013093877088419731	3.9710938710781155E-4
22	0.013566115278493886	0.0	0.0	0.001363051139532218	3.9710938710781155E-4
23	0.013931026607187551	0.0	0.0	0.001416714570222463	4.937035623502522E-4
24	0.014596453147746586	0.0	0.0	0.0015240414316029524	4.937035623502522E-4
25	0.014929166418026104	0.0	0.0	0.001631368292983442	5.044362484883011E-4
26	0.01715083244860224	0.0	0.0	0.001899685446434666	5.25901620764399E-4
27	0.01775186287233298	0.0	0.0	0.002769033023616632	5.25901620764399E-4
28	0.0185890123911008	0.0	0.0	0.0049585009957786195	5.47366993040497E-4
29	0.018760735369309584	0.0	0.0	0.009112050531203567	5.580996791785459E-4
30	0.019147112070279346	0.0	0.0	0.01486477030119781	5.688323653165949E-4
31	0.01985546935539058	0.0	0.0	0.029997857755846846	5.688323653165949E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11435	0.0	15.029734	1
TAATCCC	3325	0.0	13.409022	5
TTAATCC	3545	0.0	12.472497	4
TTTAATC	3870	0.0	11.903101	3
CGTATAC	205	2.8177055E-7	11.731707	3
CCTTTAA	4495	0.0	11.441601	1
GTATCAA	15590	0.0	11.00032	2
TAATACC	925	0.0	10.8	4
CTTTAAT	4790	0.0	10.427975	2
GGCGGGT	835	0.0	10.413174	33
TATACTG	1030	0.0	10.2378645	5
CGCAATC	455	0.0	10.164835	22
GCACTCG	2320	0.0	10.047415	13
GTATAGA	985	0.0	9.954315	1
GGGTAAG	3050	0.0	9.886886	1
GCGGGTT	950	0.0	9.736842	34
TCTACAC	1980	0.0	9.717172	3
CTAACAC	1260	0.0	9.690475	3
GTATTAG	1070	0.0	9.682242	1
GTCTTAC	920	0.0	9.652174	1
>>END_MODULE