FastQCFastQC Report
Thu 9 Feb 2017
SRR2727574.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727574.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1038169
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA4300.026.6744171
TGCGTAT450.003825563220.55555523
CTTATAC5600.020.48214137
GAATAAG852.7227048E-517.4117641
GTACTTA2950.016.93220530
TAGGAGT1107.805811E-716.8181824
TACTTAC2600.016.36538531
GTATCAA7050.016.2695032
TAGACTC1606.2937033E-1016.18755
TAAAGGT1302.588058E-715.6538464
ACCGTGC1302.588058E-715.6538468
CGTTACG3800.015.57894824
GTATAGC957.058622E-515.5789481
GTATTAA1201.9349645E-615.4166661
ATAAGAC855.3635304E-415.2352943
TCTTATA9800.014.91326537
TAATAGG750.004104051314.7999994
CTCGTTA5250.014.79999922
GTATTAG750.004104051314.7999991
ATAGTAC750.004104051314.7999993
Produced by FastQC (version 0.11.5)