FastQCFastQC Report
Thu 9 Feb 2017
SRR2727573.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727573.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2138833
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC11550.020.82251237
TCTTATA21650.018.62817637
TACTTAC6950.018.1007231
CTCGTTA7150.016.300722
CGTAGTA700.00259347215.8571432
TCGTTAC6150.015.34146323
GTATAGA2300.015.2826091
CGTTACA2450.015.10204124
GTACTTA8450.014.88757330
TCGTTAG1001.09406275E-414.823
GGTATCA12500.014.81
CATACCG750.00410594214.85
TACGGAT11350.014.66960327
TAGTACT1904.5656634E-1014.6052644
ACGGATG11500.014.47826128
CTAGTAC2059.276846E-1114.4390243
CGACTTA908.27812E-414.38888919
AGTGTAC9400.014.17021427
CGTTAGG1051.6566826E-414.09523824
CTATAGT2650.013.9622644
Produced by FastQC (version 0.11.5)