FastQCFastQC Report
Thu 9 Feb 2017
SRR2727572.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727572.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1966479
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC11700.020.08119837
TACTTAC4850.017.92783431
TCTTATA21050.016.8741137
TTAGAAC2201.8189894E-1215.1363643
TAACCGT750.004105786314.86
GTACTTA6200.014.62096830
TTTATAC1651.646913E-814.5757573
GTACCGT1406.0005914E-714.5357146
GTGTAAG2800.014.5357141
GTGTAGG3200.014.4531251
ACTTACA5000.014.42999932
TATACTG2201.8189894E-1114.2954545
GTACTAG800.00630072113.8751
TAGACAG2700.013.7037045
TTATACT2355.638867E-1113.3829794
GGTATCA13400.013.1156721
ATAGCGT850.00940783713.0588246
TAAGACT2551.6370905E-1113.0588244
ATAATAG2001.4639227E-812.9500013
TTAGACA3000.012.954
Produced by FastQC (version 0.11.5)