##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727572.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1966479
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.6934495613734	34.0	31.0	34.0	31.0	34.0
2	32.8689296961727	34.0	31.0	34.0	31.0	34.0
3	32.96036062424262	34.0	31.0	34.0	31.0	34.0
4	36.38608345169209	37.0	37.0	37.0	35.0	37.0
5	36.253021771399546	37.0	37.0	37.0	35.0	37.0
6	36.26964895124738	37.0	37.0	37.0	35.0	37.0
7	36.246893050980965	37.0	36.0	37.0	35.0	37.0
8	36.233683654897916	37.0	36.0	37.0	35.0	37.0
9	37.9655404405539	39.0	38.0	39.0	35.0	39.0
10	37.94465641382389	39.0	38.0	39.0	35.0	39.0
11	38.01831089983671	39.0	38.0	39.0	35.0	39.0
12	37.99211433226594	39.0	38.0	39.0	35.0	39.0
13	38.02534377432965	39.0	38.0	39.0	35.0	39.0
14	39.405897037293556	40.0	39.0	41.0	37.0	41.0
15	39.43056905260621	40.0	39.0	41.0	37.0	41.0
16	39.41812040708291	40.0	39.0	41.0	37.0	41.0
17	39.42718279727371	40.0	39.0	41.0	37.0	41.0
18	39.404483851594655	40.0	39.0	41.0	37.0	41.0
19	39.43871813530681	40.0	39.0	41.0	37.0	41.0
20	39.41042238437329	40.0	39.0	41.0	37.0	41.0
21	39.394199988914195	40.0	39.0	41.0	37.0	41.0
22	39.36369317953561	40.0	39.0	41.0	37.0	41.0
23	39.32768465872252	40.0	39.0	41.0	36.0	41.0
24	39.293096442931756	40.0	39.0	41.0	36.0	41.0
25	39.229528004112936	40.0	39.0	41.0	36.0	41.0
26	39.14491840492576	40.0	39.0	41.0	36.0	41.0
27	39.10369243709188	40.0	39.0	41.0	36.0	41.0
28	39.03768207033993	40.0	39.0	41.0	36.0	41.0
29	38.96335429974081	40.0	39.0	41.0	35.0	41.0
30	38.909965984889745	40.0	38.0	41.0	35.0	41.0
31	38.85193892230733	40.0	38.0	41.0	35.0	41.0
32	38.76881065091465	40.0	38.0	41.0	35.0	41.0
33	38.7318283083623	40.0	38.0	41.0	35.0	41.0
34	38.69301324855236	40.0	38.0	41.0	35.0	41.0
35	38.681180424504916	40.0	38.0	41.0	35.0	41.0
36	38.625834804236405	40.0	38.0	41.0	35.0	41.0
37	38.612166211792754	40.0	38.0	41.0	35.0	41.0
38	38.57417902759195	40.0	38.0	41.0	35.0	41.0
39	38.53407537024296	40.0	38.0	41.0	35.0	41.0
40	38.48204379502654	40.0	38.0	41.0	35.0	41.0
41	38.43781804941726	40.0	38.0	41.0	34.0	41.0
42	38.405995182252134	40.0	38.0	41.0	34.0	41.0
43	37.31834868310315	39.0	36.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	1.0
13	1.0
14	1.0
15	0.0
16	2.0
17	11.0
18	18.0
19	30.0
20	57.0
21	145.0
22	326.0
23	565.0
24	1000.0
25	1714.0
26	2759.0
27	4028.0
28	6050.0
29	8499.0
30	12387.0
31	17278.0
32	24038.0
33	33524.0
34	49698.0
35	73284.0
36	115923.0
37	202002.0
38	492161.0
39	920976.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.26901024623197	16.634197466639613	12.014417646972076	28.08237464015634
2	18.34842884159963	19.174473767581553	35.65977567011903	26.817321720699788
3	19.890779408272348	20.96859412177806	29.188666647342785	29.9519598226068
4	15.35292266024707	14.25354656724023	32.991148138373205	37.4023826341395
5	16.229260520961578	35.07883887903201	33.15051927836504	15.54138132164137
6	37.51435942107696	32.60411120586591	14.636311905695406	15.245217467361716
7	31.15456610520631	29.642218401518655	19.978194529410178	19.225020963864857
8	28.099766130225646	34.72226248030108	18.759976587596412	18.41799480187686
9	28.846176338521794	13.95585714365625	17.198861518480495	39.99910499934146
10	16.950041164945063	26.061452982716826	32.23141462481928	24.757091227518828
11	38.313554327302754	20.48656507392146	20.00733290312279	21.19254769565299
12	22.697826928230608	22.98956663152772	29.210329731464206	25.102276708777467
13	32.23665241276413	18.499155088866956	22.856486135880424	26.407706362488486
14	23.593132700628892	18.614284719033357	23.95138722559458	33.84119535474318
15	26.927976347573505	25.804394554938035	20.98329043940973	26.284338658078727
16	25.93055913640573	24.81719865810924	23.157531811933918	26.09471039355111
17	25.70421550395402	24.352103429530647	23.738417750710788	26.205263315804544
18	25.72262404022621	24.73212274323804	24.1457447549656	25.399508461570147
19	27.122588138495253	24.037022515877364	23.928910504510853	24.911478841116534
20	26.041925695621465	23.623440677474818	24.004426185074948	26.330207441828772
21	25.78669795100787	24.30384458720383	24.624773516523696	25.284683945264607
22	26.663137516342662	23.378993622611784	23.405335119266464	26.55253374177909
23	25.797173526897566	23.869718415503037	24.215870090654413	26.117237966944984
24	25.47304090203862	23.412759556547513	24.370715375043414	26.74348416637045
25	26.281948599501952	23.21387617157366	23.856751076416273	26.647424152508115
26	25.806784613514814	23.582809681669623	24.363290937762365	26.24711476705319
27	26.455100715542855	24.06636429883055	23.65578274672651	25.822752238900087
28	25.863332382395132	24.787144942814034	22.946596429455894	26.402926245334935
29	25.865468179421192	24.629604485987393	24.058889009239355	25.44603832535206
30	27.044428137803656	23.77747232490151	23.240980452880503	25.937119084414327
31	26.05235041920102	23.828731453526835	24.139235659267147	25.979682468005
32	25.633683349784054	24.069466289749343	23.959981266008942	26.33686909445766
33	25.577237285524024	23.477494547361044	24.14442259490185	26.800845572213078
34	26.11037290507552	23.646425921659983	24.83377651121624	25.40942466204826
35	25.97119013221092	23.49875081300131	24.26824797010291	26.26181108468486
36	25.68641719540356	23.19531507837104	24.886154390664736	26.232113335560665
37	25.070544867247502	23.122138604073577	25.31056777112799	26.496748757550932
38	25.776578341289174	22.500774226421946	25.10237841339775	26.620269018891126
39	24.662811044511535	22.93668022897778	26.174345111236885	26.226163615273794
40	25.860382948406773	22.071224762634127	26.171395677248523	25.896996611710577
41	24.673490029641812	22.648398482770475	26.376076225578814	26.3020352620089
42	24.348442063200267	22.74100053954301	26.70270061363483	26.207856783621896
43	23.832494524477504	22.230341641075242	26.46186407279203	27.47529976165522
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	29.0
1	34.5
2	40.0
3	97.5
4	155.0
5	155.0
6	196.5
7	238.0
8	220.5
9	203.0
10	285.0
11	367.0
12	367.0
13	622.0
14	877.0
15	1229.5
16	1582.0
17	1683.0
18	1784.0
19	1784.0
20	2309.0
21	2834.0
22	3446.0
23	4058.0
24	5432.0
25	6806.0
26	6806.0
27	8710.0
28	10614.0
29	13406.0
30	16198.0
31	20637.0
32	25076.0
33	25076.0
34	30983.5
35	36891.0
36	44028.0
37	51165.0
38	60322.5
39	69480.0
40	69480.0
41	80025.0
42	90570.0
43	103310.0
44	116050.0
45	133205.5
46	150361.0
47	150361.0
48	165991.0
49	181621.0
50	193199.0
51	204777.0
52	205220.0
53	205663.0
54	205663.0
55	196283.5
56	186904.0
57	174803.0
58	162702.0
59	151893.0
60	141084.0
61	141084.0
62	123194.0
63	105304.0
64	88896.5
65	72489.0
66	60269.0
67	48049.0
68	48049.0
69	39092.0
70	30135.0
71	23979.0
72	17823.0
73	14252.0
74	10681.0
75	10681.0
76	8552.0
77	6423.0
78	5124.5
79	3826.0
80	2915.5
81	2005.0
82	2005.0
83	1458.0
84	911.0
85	690.5
86	470.0
87	320.5
88	171.0
89	171.0
90	107.5
91	44.0
92	28.0
93	12.0
94	8.5
95	5.0
96	5.0
97	3.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1966479.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.20988873327082
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.4559990709852	52.944905257386395
2	9.409354954061538	12.083472693042834
3	3.082147853399817	5.937131121788752
4	1.5399155758171745	3.955112311274057
5	0.848535256800471	2.724217721270781
6	0.5968739727254588	2.2995126825892216
7	0.40840867307265666	1.835671281898864
8	0.3023528683731523	1.5531235229140306
9	0.21922796850583323	1.2668943118482494
>10	1.0834422754066173	11.693960626933407
>50	0.037480752322011986	1.58002459541487
>100	0.015468053475420665	1.7651381906380146
>500	7.134525492284499E-4	0.30270698360892523
>1k	7.927250546982552E-5	0.058128699391522626
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.0852310144171386E-5
2	0.0	0.0	0.0	0.0	5.0852310144171386E-5
3	0.0	0.0	0.0	0.0	5.0852310144171386E-5
4	0.0	0.0	0.0	0.0	5.0852310144171386E-5
5	0.0	5.0852310144171386E-5	0.0	0.0	5.0852310144171386E-5
6	0.0	5.0852310144171386E-5	0.0	0.0	1.5255693043251416E-4
7	0.0	5.0852310144171386E-5	0.0	0.0	1.5255693043251416E-4
8	0.0	5.0852310144171386E-5	0.0	0.0	2.0340924057668554E-4
9	0.0	5.0852310144171386E-5	0.0	0.0	2.0340924057668554E-4
10	0.0	5.0852310144171386E-5	0.0	0.0	2.0340924057668554E-4
11	0.0	5.0852310144171386E-5	0.0	0.0	2.0340924057668554E-4
12	0.0	5.0852310144171386E-5	0.0	0.0	2.0340924057668554E-4
13	0.0	5.0852310144171386E-5	0.0	0.0	2.0340924057668554E-4
14	0.0	5.0852310144171386E-5	0.0	0.0	3.0511386086502833E-4
15	0.0	5.0852310144171386E-5	0.0	5.0852310144171386E-5	4.068184811533711E-4
16	0.0	5.0852310144171386E-5	0.0	1.0170462028834277E-4	6.61080031874228E-4
17	0.0	5.0852310144171386E-5	0.0	2.0340924057668554E-4	6.61080031874228E-4
18	0.0	5.0852310144171386E-5	0.0	2.542615507208569E-4	6.61080031874228E-4
19	0.0	5.0852310144171386E-5	0.0	3.0511386086502833E-4	6.61080031874228E-4
20	0.0	5.0852310144171386E-5	0.0	4.5767079129754244E-4	6.61080031874228E-4
21	0.0	5.0852310144171386E-5	0.0	7.119323420183994E-4	7.119323420183994E-4
22	0.0	5.0852310144171386E-5	0.0	0.0012204554434601133	7.119323420183994E-4
23	0.0	5.0852310144171386E-5	0.0	0.0015255693043251415	8.136369623067422E-4
24	0.0	5.0852310144171386E-5	0.0	0.0020340924057668554	8.136369623067422E-4
25	0.0	5.0852310144171386E-5	0.0	0.0025934678173527405	8.136369623067422E-4
26	0.0	5.0852310144171386E-5	0.0	0.003966480191245368	8.136369623067422E-4
27	0.0	5.0852310144171386E-5	0.0	0.009356825066527534	8.644892724509136E-4
28	0.0	5.0852310144171386E-5	0.0	0.03788497105740768	8.644892724509136E-4
29	0.0	5.0852310144171386E-5	0.0	0.08283841322485519	8.644892724509136E-4
30	0.0	5.0852310144171386E-5	0.0	0.1476751086586737	8.644892724509136E-4
31	0.0	5.0852310144171386E-5	0.0	0.47958813696968033	9.153415825950849E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	1170	0.0	20.081198	37
TACTTAC	485	0.0	17.927834	31
TCTTATA	2105	0.0	16.87411	37
TTAGAAC	220	1.8189894E-12	15.136364	3
TAACCGT	75	0.0041057863	14.8	6
GTACTTA	620	0.0	14.620968	30
TTTATAC	165	1.646913E-8	14.575757	3
GTACCGT	140	6.0005914E-7	14.535714	6
GTGTAAG	280	0.0	14.535714	1
GTGTAGG	320	0.0	14.453125	1
ACTTACA	500	0.0	14.429999	32
TATACTG	220	1.8189894E-11	14.295454	5
GTACTAG	80	0.006300721	13.875	1
TAGACAG	270	0.0	13.703704	5
TTATACT	235	5.638867E-11	13.382979	4
GGTATCA	1340	0.0	13.115672	1
ATAGCGT	85	0.009407837	13.058824	6
TAAGACT	255	1.6370905E-11	13.058824	4
ATAATAG	200	1.4639227E-8	12.950001	3
TTAGACA	300	0.0	12.95	4
>>END_MODULE