FastQCFastQC Report
Thu 9 Feb 2017
SRR2727571.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727571.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1538251
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC11550.020.34199137
TACTTAC5700.018.8245631
CGACTTA1502.5102054E-1017.26666619
TACCGTC1107.811832E-716.8181827
GTATTAT1005.8803944E-616.651
ATACCGT1005.8803944E-616.656
GCTTATA2450.015.8571421
GTATAAA957.062161E-515.5789461
GTACTTA6800.015.50735330
GTTATAC1201.9364397E-615.4166663
CTCGTTA6750.015.34814722
AGTGTAC7600.015.33552527
ACTTACA4900.015.1020432
TCGTTAC5950.014.92436923
GGTATCA12500.014.81
GTACCGT1252.9601379E-614.7999996
GTGTACT7550.014.70198528
TCTTATA18450.014.53929537
ATCTCGT7650.014.50980420
CGTTACG4850.014.49484524
Produced by FastQC (version 0.11.5)