##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727571.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1538251
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.65032039634624	34.0	31.0	34.0	31.0	34.0
2	32.82404627073215	34.0	31.0	34.0	31.0	34.0
3	32.917991277106275	34.0	31.0	34.0	31.0	34.0
4	36.35757688439663	37.0	37.0	37.0	35.0	37.0
5	36.218498151472026	37.0	35.0	37.0	35.0	37.0
6	36.23838112245661	37.0	36.0	37.0	35.0	37.0
7	36.21297759598401	37.0	36.0	37.0	35.0	37.0
8	36.202966225928016	37.0	36.0	37.0	35.0	37.0
9	37.934600400064745	39.0	38.0	39.0	35.0	39.0
10	37.90235078670516	39.0	38.0	39.0	35.0	39.0
11	37.983106138074994	39.0	38.0	39.0	35.0	39.0
12	37.956704074952654	39.0	38.0	39.0	35.0	39.0
13	37.99098911686064	39.0	38.0	39.0	35.0	39.0
14	39.351048040924404	40.0	39.0	41.0	37.0	41.0
15	39.37474638404266	40.0	39.0	41.0	37.0	41.0
16	39.3554114380553	40.0	39.0	41.0	36.0	41.0
17	39.37639761001293	40.0	39.0	41.0	37.0	41.0
18	39.34130645778875	40.0	39.0	41.0	36.0	41.0
19	39.388136266448065	40.0	39.0	41.0	37.0	41.0
20	39.35713872443444	40.0	39.0	41.0	37.0	41.0
21	39.34504186897977	40.0	39.0	41.0	36.0	41.0
22	39.31833881466679	40.0	39.0	41.0	36.0	41.0
23	39.28015258888179	40.0	39.0	41.0	36.0	41.0
24	39.240014145935874	40.0	39.0	41.0	36.0	41.0
25	39.18146843395519	40.0	39.0	41.0	36.0	41.0
26	39.09070756333004	40.0	39.0	41.0	36.0	41.0
27	39.05198176370436	40.0	39.0	41.0	36.0	41.0
28	38.96366002687468	40.0	38.0	41.0	36.0	41.0
29	38.89195716433794	40.0	38.0	41.0	35.0	41.0
30	38.850831236254685	40.0	38.0	41.0	35.0	41.0
31	38.78504483338545	40.0	38.0	41.0	35.0	41.0
32	38.69931630143585	40.0	38.0	41.0	35.0	41.0
33	38.660962352697965	40.0	38.0	41.0	35.0	41.0
34	38.62091037158435	40.0	38.0	41.0	35.0	41.0
35	38.622583700579426	40.0	38.0	41.0	35.0	41.0
36	38.54806400255875	40.0	38.0	41.0	35.0	41.0
37	38.55098420218807	40.0	38.0	41.0	35.0	41.0
38	38.52284575144108	40.0	38.0	41.0	35.0	41.0
39	38.47323941281364	40.0	38.0	41.0	35.0	41.0
40	38.4343068848972	40.0	38.0	41.0	34.0	41.0
41	38.37911498188527	40.0	38.0	41.0	34.0	41.0
42	38.35818796802342	40.0	38.0	41.0	34.0	41.0
43	37.27149665431714	39.0	36.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	0.0
12	1.0
13	0.0
14	2.0
15	2.0
16	1.0
17	9.0
18	16.0
19	36.0
20	69.0
21	119.0
22	236.0
23	461.0
24	781.0
25	1302.0
26	2011.0
27	3202.0
28	4631.0
29	6913.0
30	9742.0
31	13881.0
32	19325.0
33	27368.0
34	40275.0
35	60440.0
36	94301.0
37	165242.0
38	402911.0
39	684971.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.20276729870483	16.41357619790268	12.066821344500996	28.316835158891497
2	18.234930450232113	19.11528092619475	35.9385431896355	26.711245433937634
3	19.80372513978538	20.907576201803217	29.602451095432407	29.686247562978995
4	15.547202634680557	14.285444963143206	32.98128848932976	37.18606391284647
5	16.26633104740384	35.185870186335	33.13360433375308	15.414194432508088
6	37.51552900014367	32.44210470202847	14.760399960734627	15.28196633709323
7	30.98089973612889	30.081371635708347	19.89356743470344	19.044161193459324
8	28.029365818712293	35.16344211705372	18.81604497575493	17.99114708847906
9	29.521645037123328	13.816795828509132	16.94274861514798	39.71881051921955
10	16.83359867797908	26.272045329403326	32.233556162160795	24.6607998304568
11	38.481366174961046	20.486838623865676	19.874389810245532	21.157405390927746
12	22.642858675209702	22.89873369170571	29.150509247190477	25.307898385894113
13	32.25715439157849	18.52252980820425	22.783212882682996	26.437102917534265
14	23.470551945033677	18.67484565262756	24.027613178863525	33.82698922347524
15	27.246463678554413	25.78798908630646	20.95087212685056	26.01467510828857
16	25.713878944333533	25.05676901884023	23.426930975504	25.80242106132224
17	25.807231719660834	24.132992600037316	23.559354097608256	26.50042158269359
18	25.521972681961525	25.229562665650796	24.1532103668387	25.09525428554898
19	27.48394117735012	24.083065767550288	23.815294123000733	24.617698932098858
20	25.886477564454697	23.49525532569132	23.975411035000143	26.642856074853842
21	25.56474853583713	24.50952412837697	25.02159920585132	24.904128129934584
22	26.83073178564487	23.056900336811093	23.161239615641403	26.95112826190264
23	25.708613223719667	23.891679576350022	24.45394152189727	25.94576567803304
24	25.082642559634287	23.2781906203864	24.70416076440061	26.935006055578707
25	26.094896086529438	22.913620728996765	23.88400852656686	27.107474657906934
26	25.58821674746189	23.476012692336944	24.653063771777166	26.282706788423997
27	26.734453609976526	23.997709086488488	23.649521437008655	25.618315866526338
28	25.57235457672383	25.151942043268622	22.646889226790687	26.62881415321687
29	25.733121577687907	24.737315301599025	24.18194429907733	25.34761882163574
30	27.5299674760491	23.53244041447072	23.006713468738198	25.930878640741984
31	26.091580632809602	23.9135225655631	24.21314856938172	25.781748232245583
32	25.732796533205573	24.04224018056871	23.863530724179604	26.361432562046115
33	25.524182984441417	23.24490606539505	24.05543698655161	27.175473963611918
34	26.08611988550633	23.659662824857584	25.173785032481693	25.08043225715439
35	26.10887299926995	23.234049579684978	24.232326193839626	26.424751227205444
36	25.3599055030681	23.03421223194394	25.33617725585746	26.2697050091305
37	24.775475523825435	23.04643390447983	25.858523738973677	26.31956683272106
38	25.909620731597123	22.107705439489393	25.22826248772144	26.754411341192043
39	24.190070411135764	22.98135999911588	26.76864828951842	26.059921300229938
40	26.042238880390784	21.64367193650451	26.47539315755361	25.838696025551094
41	24.60944280224749	22.645589048861336	26.579797445280384	26.16517070361079
42	24.146124397123746	22.591111593621587	27.133023154218655	26.12974085503601
43	23.390656011275144	22.07786635601082	26.608661395311948	27.92281623740209
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	15.0
1	24.0
2	33.0
3	64.0
4	95.0
5	95.0
6	130.5
7	166.0
8	163.0
9	160.0
10	214.0
11	268.0
12	268.0
13	438.5
14	609.0
15	943.0
16	1277.0
17	1324.5
18	1372.0
19	1372.0
20	1665.5
21	1959.0
22	2464.0
23	2969.0
24	3962.5
25	4956.0
26	4956.0
27	6493.0
28	8030.0
29	9931.5
30	11833.0
31	15395.5
32	18958.0
33	18958.0
34	23681.0
35	28404.0
36	34028.0
37	39652.0
38	46790.5
39	53929.0
40	53929.0
41	61908.5
42	69888.0
43	80017.5
44	90147.0
45	104018.5
46	117890.0
47	117890.0
48	132376.0
49	146862.0
50	157667.5
51	168473.0
52	168445.0
53	168417.0
54	168417.0
55	157228.0
56	146039.0
57	135475.0
58	124911.0
59	116236.0
60	107561.0
61	107561.0
62	93769.0
63	79977.0
64	66776.0
65	53575.0
66	44339.5
67	35104.0
68	35104.0
69	28658.0
70	22212.0
71	17905.5
72	13599.0
73	11007.5
74	8416.0
75	8416.0
76	6671.0
77	4926.0
78	3911.5
79	2897.0
80	2214.5
81	1532.0
82	1532.0
83	1093.0
84	654.0
85	477.0
86	300.0
87	208.5
88	117.0
89	117.0
90	80.5
91	44.0
92	28.0
93	12.0
94	10.0
95	8.0
96	8.0
97	5.0
98	2.0
99	2.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1538251.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	67.93272449336735
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.66860313031233	57.51768889689768
2	8.565143660726322	11.637070891004662
3	2.6803032543228444	5.462409076037691
4	1.3074690614452231	3.552797421390397
5	0.7214117059074253	2.4503731331849634
6	0.4752896066993784	1.9372630743881878
7	0.339844666376179	1.6160601864031319
8	0.2267870833685847	1.2325011562505916
9	0.16936837752514777	1.0355089795474044
>10	0.7940620588999395	8.923967784957332
>50	0.030867461159560072	1.4304261928612734
>100	0.01951103208281502	2.555877374361698
>500	0.001147629577895743	0.4983045851552413
>1k	1.9127159631595716E-4	0.14975124755969116
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	6.500889646748157E-5
4	0.0	0.0	0.0	6.500889646748157E-5	6.500889646748157E-5
5	0.0	0.0	0.0	6.500889646748157E-5	6.500889646748157E-5
6	0.0	0.0	0.0	6.500889646748157E-5	6.500889646748157E-5
7	0.0	0.0	0.0	6.500889646748157E-5	6.500889646748157E-5
8	0.0	0.0	0.0	6.500889646748157E-5	6.500889646748157E-5
9	0.0	0.0	0.0	1.3001779293496314E-4	6.500889646748157E-5
10	0.0	0.0	0.0	1.9502668940244472E-4	6.500889646748157E-5
11	0.0	0.0	0.0	2.600355858699263E-4	6.500889646748157E-5
12	0.0	0.0	0.0	2.600355858699263E-4	6.500889646748157E-5
13	0.0	0.0	0.0	2.600355858699263E-4	6.500889646748157E-5
14	0.0	0.0	0.0	3.250444823374079E-4	1.3001779293496314E-4
15	0.0	0.0	0.0	3.9005337880488943E-4	1.3001779293496314E-4
16	0.0	0.0	0.0	5.200711717398526E-4	1.9502668940244472E-4
17	0.0	0.0	0.0	6.500889646748158E-4	1.9502668940244472E-4
18	0.0	0.0	0.0	6.500889646748158E-4	1.9502668940244472E-4
19	0.0	0.0	0.0	7.150978611422974E-4	1.9502668940244472E-4
20	0.0	0.0	0.0	7.150978611422974E-4	1.9502668940244472E-4
21	0.0	0.0	0.0	9.101245505447421E-4	2.600355858699263E-4
22	0.0	0.0	0.0	0.0011701601364146684	2.600355858699263E-4
23	0.0	0.0	0.0	0.0016252224116870394	3.250444823374079E-4
24	0.0	0.0	0.0	0.002145293583426892	3.250444823374079E-4
25	0.0	0.0	0.0	0.002600355858699263	3.250444823374079E-4
26	0.0	0.0	0.0	0.003965542684516376	3.250444823374079E-4
27	0.0	0.0	0.0	0.011311547985341794	3.250444823374079E-4
28	0.0	0.0	0.0	0.04466111187315984	3.250444823374079E-4
29	0.0	0.0	0.0	0.09855348704470207	3.250444823374079E-4
30	0.0	0.0	0.0	0.17558902935866774	3.250444823374079E-4
31	0.0	0.0	0.0	0.5688278440904638	3.250444823374079E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	1155	0.0	20.341991	37
TACTTAC	570	0.0	18.82456	31
CGACTTA	150	2.5102054E-10	17.266666	19
TACCGTC	110	7.811832E-7	16.818182	7
GTATTAT	100	5.8803944E-6	16.65	1
ATACCGT	100	5.8803944E-6	16.65	6
GCTTATA	245	0.0	15.857142	1
GTATAAA	95	7.062161E-5	15.578946	1
GTACTTA	680	0.0	15.507353	30
GTTATAC	120	1.9364397E-6	15.416666	3
CTCGTTA	675	0.0	15.348147	22
AGTGTAC	760	0.0	15.335525	27
ACTTACA	490	0.0	15.10204	32
TCGTTAC	595	0.0	14.924369	23
GGTATCA	1250	0.0	14.8	1
GTACCGT	125	2.9601379E-6	14.799999	6
GTGTACT	755	0.0	14.701985	28
TCTTATA	1845	0.0	14.539295	37
ATCTCGT	765	0.0	14.509804	20
CGTTACG	485	0.0	14.494845	24
>>END_MODULE