FastQCFastQC Report
Thu 9 Feb 2017
SRR2727570.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727570.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1606569
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTATA11000.017.4909137
CTTATAC6000.017.26666637
ATTAGAC650.00158030417.0769233
TACTTAC3150.015.85714331
GTATTAT957.062471E-515.5789481
TATTCCG855.3658016E-415.2352935
TATACCG1001.0937943E-414.7999995
ATAGACC908.27654E-414.3888884
GAGTAAT1051.6562776E-414.0952391
ATACCGG1051.6562776E-414.0952396
GTACTTA3450.013.94202830
GTATATA1203.301964E-513.8750011
ATTATAC1203.301964E-513.8750013
TATACTG2009.840733E-1013.8749995
CGTGTAA950.00124545113.6315791
CTCTTAT18400.013.47282537
AGGGCGT4000.013.41249910
TAGACAG3050.013.3442615
TTATACT1951.0264557E-813.282054
TAGACCG850.0094068613.0588235
Produced by FastQC (version 0.11.5)