##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727570.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1606569
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.189528118618	31.0	31.0	34.0	30.0	34.0
2	31.36787464466201	31.0	31.0	34.0	30.0	34.0
3	31.4653208172198	31.0	31.0	34.0	30.0	34.0
4	35.30561214613253	37.0	35.0	37.0	33.0	37.0
5	34.355025523335755	35.0	35.0	37.0	30.0	37.0
6	34.31000722658037	35.0	35.0	37.0	30.0	37.0
7	34.293708517965925	35.0	35.0	37.0	30.0	37.0
8	34.197688365703556	35.0	35.0	37.0	30.0	37.0
9	35.796943050687524	37.0	35.0	39.0	30.0	39.0
10	35.491262435662584	37.0	35.0	39.0	30.0	39.0
11	35.67308220188489	37.0	35.0	39.0	30.0	39.0
12	35.60146311798622	37.0	35.0	39.0	30.0	39.0
13	35.74277108546225	37.0	35.0	39.0	30.0	39.0
14	36.68372102287546	38.0	36.0	40.0	31.0	41.0
15	36.63626772332841	38.0	36.0	40.0	31.0	41.0
16	36.59636467528005	38.0	36.0	40.0	31.0	41.0
17	36.579225044177996	38.0	36.0	40.0	31.0	41.0
18	36.51932721221435	38.0	36.0	40.0	31.0	41.0
19	36.572811998737684	38.0	35.0	40.0	31.0	41.0
20	36.53887508099559	38.0	35.0	40.0	31.0	41.0
21	36.49056903251588	38.0	35.0	40.0	30.0	41.0
22	36.47257229536982	38.0	35.0	40.0	30.0	41.0
23	36.38691273141708	38.0	35.0	40.0	30.0	41.0
24	36.29532064915979	38.0	35.0	40.0	30.0	41.0
25	36.18683542381311	38.0	35.0	40.0	30.0	41.0
26	35.99179369202319	38.0	34.0	40.0	29.0	41.0
27	35.87961861582042	38.0	34.0	40.0	29.0	41.0
28	35.75409023826552	38.0	34.0	40.0	29.0	41.0
29	35.602747220941026	38.0	34.0	40.0	28.0	41.0
30	35.506572080004034	38.0	34.0	40.0	27.0	41.0
31	35.39599295143875	38.0	34.0	40.0	27.0	41.0
32	35.24721814002386	38.0	34.0	40.0	27.0	41.0
33	35.184082351894006	38.0	34.0	40.0	27.0	40.0
34	35.09129330890861	38.0	33.0	40.0	27.0	40.0
35	35.086059173306595	38.0	33.0	40.0	27.0	40.0
36	35.008424785988026	38.0	33.0	40.0	26.0	40.0
37	34.949785536755655	38.0	33.0	40.0	26.0	40.0
38	34.896750777588764	37.0	33.0	40.0	26.0	40.0
39	34.83117874177829	37.0	33.0	39.0	26.0	40.0
40	34.740300603335434	37.0	33.0	39.0	26.0	40.0
41	34.70621616625243	37.0	33.0	39.0	26.0	40.0
42	34.646442200739585	37.0	33.0	39.0	26.0	40.0
43	33.46659620595194	36.0	31.0	39.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	2.0
12	1.0
13	3.0
14	10.0
15	22.0
16	58.0
17	88.0
18	217.0
19	411.0
20	859.0
21	1433.0
22	2590.0
23	4107.0
24	6405.0
25	9770.0
26	14315.0
27	20045.0
28	28082.0
29	38313.0
30	51347.0
31	67117.0
32	88526.0
33	115410.0
34	151260.0
35	195261.0
36	240144.0
37	263648.0
38	228460.0
39	78664.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.19383045483885	17.980927056354258	12.376250257536402	27.44899223127049
2	19.274553411649297	20.248679017209966	34.236189046346595	26.240578524794145
3	20.655321993639863	21.840767498937176	28.846566814123765	28.657343693299197
4	16.16158409629465	15.097204041656473	32.912311889498675	35.8288999725502
5	16.24144372261633	34.96009197239584	33.18568950353206	15.612774801455773
6	37.19852679841327	33.21394848276047	14.506255255765549	15.081269463060721
7	30.956715833555858	29.321180727376166	20.165769412953942	19.556334026114037
8	27.916385788596692	34.39254709881742	18.766078518880917	18.92498859370497
9	28.138411733327356	14.01010476362982	17.481539852941268	40.369943650101554
10	16.98588731638666	26.132148697005857	32.51618822471988	24.3657757618876
11	38.24329985204495	20.48576811826943	20.084291430993627	21.186640598691994
12	22.619009827775837	23.443313047867846	29.186608231579225	24.75106889277709
13	32.13189100499263	18.746596006769707	22.933655510594317	26.18785747764335
14	23.556784675914948	18.799566031711056	24.0184517440583	33.6251975483157
15	26.637075656258773	26.10525909562552	20.92060782948009	26.337057418635613
16	26.009278157365166	24.761525959980553	23.14292134355885	26.086274539095427
17	25.61607998162544	24.63821970920639	23.874853803353606	25.870846505814566
18	25.745672921611213	24.549272393529318	24.257159200756394	25.44789548410308
19	26.8256140881593	24.169394529584476	24.08262576957479	24.922365612681435
20	26.12785383011872	23.875165025591805	24.029406766842882	25.967574377446596
21	25.801506191143986	24.343181027394404	24.452855744135483	25.402457037326126
22	26.43341182358181	23.700195883276724	23.73959661863263	26.126795674508845
23	25.935829709150372	23.928135050533157	24.109266393164564	26.02676884715191
24	25.75301776643269	23.81765115597276	24.147795706253515	26.281535371341036
25	26.259936548010078	23.504499339897635	23.88338129268024	26.35218281941205
26	25.93626542028385	23.832029623377522	24.155514017760833	26.076190938577803
27	26.327720751489665	24.171448596356583	23.771465775824133	25.72936487632962
28	26.05079520394082	24.43405792094831	23.41947342442186	26.095673450689016
29	25.969628444218706	24.46630054482565	24.0627075463301	25.50136346462555
30	26.580059742220847	24.176614885510674	23.515765584920413	25.72755978734807
31	26.130530341367226	23.878837448002542	24.080011502773925	25.910620707856307
32	25.60873513680396	24.194416797535617	23.997911076337214	26.198936989323208
33	25.70309771942568	23.825431711927717	24.07341359132412	26.398056977322483
34	26.108495806902788	23.783727932009143	24.672329666512923	25.435446594575144
35	26.005294512716233	23.748933285778577	24.277264157344007	25.968508044161194
36	25.86586695000339	23.53026854122045	24.5818884840925	26.021976024683656
37	25.313758699439614	23.257513371663464	25.145200735231416	26.283527193665506
38	25.66431942854617	22.951644155962178	24.989714105027545	26.3943223104641
39	24.95759597004548	23.258633771720977	25.726314898395273	26.057455359838265
40	25.733846476559673	22.567533669577838	25.87352301706307	25.825096836799418
41	24.901638211617428	22.89020888614183	26.03822182551761	26.16993107672313
42	24.501593146637337	22.964777734414145	26.311848417341555	26.221780701606967
43	24.255291867327205	22.558943935803565	26.16239949855873	27.0233646983105
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	20.0
1	27.5
2	35.0
3	70.5
4	106.0
5	106.0
6	148.5
7	191.0
8	200.0
9	209.0
10	290.5
11	372.0
12	372.0
13	577.0
14	782.0
15	1178.5
16	1575.0
17	1633.0
18	1691.0
19	1691.0
20	2184.5
21	2678.0
22	3104.0
23	3530.0
24	4783.5
25	6037.0
26	6037.0
27	8109.5
28	10182.0
29	12462.0
30	14742.0
31	18670.5
32	22599.0
33	22599.0
34	27768.0
35	32937.0
36	38784.0
37	44631.0
38	52751.0
39	60871.0
40	60871.0
41	69352.5
42	77834.0
43	87946.5
44	98059.0
45	109542.0
46	121025.0
47	121025.0
48	132841.5
49	144658.0
50	152038.0
51	159418.0
52	160636.0
53	161854.0
54	161854.0
55	155842.0
56	149830.0
57	140149.5
58	130469.0
59	121472.5
60	112476.0
61	112476.0
62	98898.0
63	85320.0
64	72328.5
65	59337.0
66	49484.5
67	39632.0
68	39632.0
69	32523.0
70	25414.0
71	20517.5
72	15621.0
73	12623.5
74	9626.0
75	9626.0
76	7790.0
77	5954.0
78	4697.5
79	3441.0
80	2700.0
81	1959.0
82	1959.0
83	1413.0
84	867.0
85	634.0
86	401.0
87	271.5
88	142.0
89	142.0
90	85.5
91	29.0
92	21.0
93	13.0
94	7.0
95	1.0
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1606569.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	71.03400278832858
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.44153638689309	60.69254333945642
2	8.034039096373464	11.413799111466668
3	2.621427862582013	5.586315423001588
4	1.2263633000306842	3.4845397629553387
5	0.7376240380892356	2.6198193989184473
6	0.4532411342862734	1.931731919800581
7	0.31517390888257335	1.567164502566119
8	0.23322220212273087	1.3253365244708957
9	0.16219264319154353	1.0369073401843036
>10	0.7505315853548594	8.573382816698322
>50	0.018503405471034143	0.873942110887275
>100	0.005793366981723382	0.7515613011929881
>500	3.5106974083207697E-4	0.14295644840108004
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.224444763965943E-5
2	0.0	0.0	0.0	0.0	6.224444763965943E-5
3	0.0	0.0	0.0	0.0	6.224444763965943E-5
4	0.0	0.0	0.0	6.224444763965943E-5	1.2448889527931885E-4
5	0.0	0.0	0.0	6.224444763965943E-5	1.2448889527931885E-4
6	0.0	0.0	0.0	6.224444763965943E-5	1.2448889527931885E-4
7	0.0	0.0	0.0	6.224444763965943E-5	1.2448889527931885E-4
8	0.0	0.0	0.0	6.224444763965943E-5	1.8673334291897828E-4
9	0.0	0.0	0.0	1.2448889527931885E-4	1.8673334291897828E-4
10	0.0	0.0	0.0	1.2448889527931885E-4	1.8673334291897828E-4
11	0.0	0.0	0.0	1.2448889527931885E-4	1.8673334291897828E-4
12	0.0	0.0	0.0	1.2448889527931885E-4	1.8673334291897828E-4
13	0.0	0.0	0.0	1.2448889527931885E-4	1.8673334291897828E-4
14	0.0	0.0	0.0	1.2448889527931885E-4	3.1122223819829713E-4
15	0.0	0.0	0.0	1.2448889527931885E-4	3.7346668583795655E-4
16	0.0	0.0	0.0	2.489777905586377E-4	3.7346668583795655E-4
17	0.0	0.0	0.0	3.1122223819829713E-4	3.7346668583795655E-4
18	0.0	0.0	0.0	3.1122223819829713E-4	3.7346668583795655E-4
19	0.0	0.0	0.0	4.35711133477616E-4	3.7346668583795655E-4
20	0.0	0.0	0.0	4.35711133477616E-4	3.7346668583795655E-4
21	6.224444763965943E-5	0.0	0.0	4.979555811172754E-4	4.35711133477616E-4
22	6.224444763965943E-5	0.0	0.0	7.469333716759131E-4	4.35711133477616E-4
23	6.224444763965943E-5	0.0	0.0	9.336667145948914E-4	4.979555811172754E-4
24	6.224444763965943E-5	0.0	0.0	0.001307133400432848	4.979555811172754E-4
25	6.224444763965943E-5	6.224444763965943E-5	0.0	0.0015561111909914856	4.979555811172754E-4
26	6.224444763965943E-5	6.224444763965943E-5	0.0	0.0021785556673880797	5.602000287569348E-4
27	6.224444763965943E-5	6.224444763965943E-5	0.0	0.008216267088435043	5.602000287569348E-4
28	6.224444763965943E-5	6.224444763965943E-5	0.0	0.032055890534424605	5.602000287569348E-4
29	6.224444763965943E-5	6.224444763965943E-5	0.0	0.06772195903194946	5.602000287569348E-4
30	6.224444763965943E-5	6.224444763965943E-5	0.0	0.11639711708616313	5.602000287569348E-4
31	6.224444763965943E-5	6.224444763965943E-5	0.0	0.3493158401537687	5.602000287569348E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTTATA	1100	0.0	17.49091	37
CTTATAC	600	0.0	17.266666	37
ATTAGAC	65	0.001580304	17.076923	3
TACTTAC	315	0.0	15.857143	31
GTATTAT	95	7.062471E-5	15.578948	1
TATTCCG	85	5.3658016E-4	15.235293	5
TATACCG	100	1.0937943E-4	14.799999	5
ATAGACC	90	8.27654E-4	14.388888	4
GAGTAAT	105	1.6562776E-4	14.095239	1
ATACCGG	105	1.6562776E-4	14.095239	6
GTACTTA	345	0.0	13.942028	30
GTATATA	120	3.301964E-5	13.875001	1
ATTATAC	120	3.301964E-5	13.875001	3
TATACTG	200	9.840733E-10	13.874999	5
CGTGTAA	95	0.001245451	13.631579	1
CTCTTAT	1840	0.0	13.472825	37
AGGGCGT	400	0.0	13.412499	10
TAGACAG	305	0.0	13.344261	5
TTATACT	195	1.0264557E-8	13.28205	4
TAGACCG	85	0.00940686	13.058823	5
>>END_MODULE