FastQCFastQC Report
Thu 9 Feb 2017
SRR2727569.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727569.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1149569
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATACG502.7207898E-729.61
TTAGTAC400.001931187723.1253
TACATAT957.1449904E-921.42105335
CTTATAC8100.020.7839537
TTACATA1052.2593667E-819.3809534
TCGTTAC1850.018.023
TCTATAC1054.797257E-717.6190473
CCTTATA953.6063884E-617.5263162
CTCGTTA2550.016.68627422
GTATATA904.445959E-516.4444451
CGTTACG2150.016.34883724
TCTTATA13350.016.21348437
TAGACCG700.002592494215.8571425
GCTTATA1403.4726327E-815.8571421
GTGCGAT700.002592494215.85714211
ATAGACT1302.5887675E-715.6538484
TACTTAC2250.015.62222231
ATCTCGT3200.015.60937520
TATACTG1455.3445547E-815.3103455
TCTCGTT2850.014.92982421
Produced by FastQC (version 0.11.5)