##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727569.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1149569
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.47632808469957	34.0	31.0	34.0	31.0	34.0
2	32.65424693950516	34.0	31.0	34.0	31.0	34.0
3	32.75307876256232	34.0	31.0	34.0	31.0	34.0
4	36.24185325108802	37.0	37.0	37.0	35.0	37.0
5	36.08255180854738	37.0	35.0	37.0	35.0	37.0
6	36.09968344657867	37.0	35.0	37.0	35.0	37.0
7	36.07003581342225	37.0	35.0	37.0	35.0	37.0
8	36.05503366914035	37.0	35.0	37.0	35.0	37.0
9	37.759863044323566	39.0	37.0	39.0	35.0	39.0
10	37.71763765376415	39.0	37.0	39.0	35.0	39.0
11	37.80325495903247	39.0	37.0	39.0	35.0	39.0
12	37.76620542133617	39.0	37.0	39.0	35.0	39.0
13	37.803418498585124	39.0	37.0	39.0	35.0	39.0
14	39.13406850741452	40.0	39.0	41.0	36.0	41.0
15	39.149464712427005	40.0	39.0	41.0	36.0	41.0
16	39.12910229833964	40.0	38.0	41.0	36.0	41.0
17	39.13590397792564	40.0	38.0	41.0	36.0	41.0
18	39.10732196153515	40.0	38.0	41.0	36.0	41.0
19	39.14346594245322	40.0	39.0	41.0	36.0	41.0
20	39.12389860895692	40.0	39.0	41.0	36.0	41.0
21	39.101812940328074	40.0	39.0	41.0	36.0	41.0
22	39.069544324873064	40.0	39.0	41.0	36.0	41.0
23	39.02979290499309	40.0	39.0	41.0	36.0	41.0
24	38.983090184234264	40.0	38.0	41.0	36.0	41.0
25	38.92335127339029	40.0	38.0	41.0	35.0	41.0
26	38.816346822156824	40.0	38.0	41.0	35.0	41.0
27	38.77693900931567	40.0	38.0	41.0	35.0	41.0
28	38.709140556156264	40.0	38.0	41.0	35.0	41.0
29	38.63169065971682	40.0	38.0	41.0	35.0	41.0
30	38.566125217364075	40.0	38.0	41.0	35.0	41.0
31	38.50487269576685	40.0	38.0	41.0	34.0	41.0
32	38.41519212852817	40.0	38.0	41.0	34.0	41.0
33	38.368824315895786	40.0	38.0	41.0	34.0	41.0
34	38.327126949317524	40.0	38.0	41.0	34.0	41.0
35	38.313174763759285	40.0	38.0	41.0	34.0	41.0
36	38.263866718744154	40.0	38.0	41.0	34.0	41.0
37	38.2484574653631	40.0	38.0	41.0	34.0	41.0
38	38.20514732043053	40.0	38.0	41.0	34.0	41.0
39	38.166481524814955	40.0	38.0	41.0	34.0	41.0
40	38.114256734480485	40.0	38.0	41.0	33.0	41.0
41	38.07214268999947	40.0	38.0	41.0	33.0	41.0
42	38.03201460721366	40.0	38.0	41.0	33.0	41.0
43	36.92533984475921	39.0	36.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	1.0
13	0.0
14	1.0
15	1.0
16	3.0
17	9.0
18	11.0
19	25.0
20	69.0
21	116.0
22	277.0
23	439.0
24	861.0
25	1374.0
26	2112.0
27	3266.0
28	4728.0
29	6803.0
30	9668.0
31	13310.0
32	18195.0
33	24706.0
34	35750.0
35	51154.0
36	78418.0
37	134758.0
38	317790.0
39	445723.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.357380026775246	16.56151131424038	11.934559821985458	28.146548836998907
2	18.384194424171145	19.140999800794905	35.04635215459011	27.42845362044384
3	19.95147746677233	20.865037244393335	29.0703733312224	30.113111957611938
4	15.42673819492349	14.277002946321621	32.94104138159606	37.35521747715883
5	16.362306220853206	34.76807394771432	33.21949356671936	15.650126264713125
6	37.838529048713035	32.61892065635033	14.447327650623842	15.09522264431278
7	31.531295642105867	29.29767591158077	19.82386442223129	19.34716402408207
8	28.518340351905803	34.26545079068764	18.617673232315763	18.598535625090797
9	28.69031784955927	13.785514397134927	17.035080103934604	40.4890876493712
10	16.99471715051467	25.836204699326444	32.24947784778469	24.919600302374196
11	38.62612857514425	20.204180871265663	19.86675006023997	21.302940493350118
12	23.031762338754785	22.94999256242992	28.979382707780044	25.03886239103525
13	32.46608076592183	18.19412318877771	22.78732290101769	26.552473144282768
14	23.744986164379867	18.30807894089002	23.891127892279627	34.055807002450486
15	27.03621966145573	25.517476549906963	20.778483066262226	26.66782072237508
16	26.403721742670516	24.285014644618983	22.963562865734897	26.347700746975605
17	25.897792998941345	24.203766803036615	23.59919239297511	26.29924780504693
18	26.103870233104754	24.23864944166031	24.114168005574264	25.54331231966067
19	27.24777721041538	23.86677093762967	23.7270664048874	25.158385447067555
20	26.431819229641718	23.495849313960278	23.79143835646229	26.280893099935714
21	26.065073083912317	24.187151880400393	24.028309740433155	25.719465295254135
22	26.816485134863587	23.336311261003036	23.2357518339482	26.611451770185173
23	26.19138129159711	23.58979756761012	23.734460480406135	26.48436066038663
24	25.94346228891002	23.255237397668168	23.887735316453384	26.913564996968432
25	26.488623127450374	22.895972316581258	23.594494980292612	27.020909575675756
26	26.139622762965946	23.381893561847964	24.068672693853088	26.409810981333003
27	26.670256417840076	23.723934796432403	23.448353252392852	26.157455533334666
28	26.291853729528196	24.23769256129906	22.99896743910109	26.471486270071654
29	26.2293955386758	24.101728560878033	23.87686167598465	25.792014224461518
30	26.965497503847097	23.670001539707492	23.197824575993266	26.166676380452152
31	26.244009711465775	23.56022126553517	23.84189204823721	26.353876974761846
32	25.802800875806497	23.752554218146106	23.709929547508676	26.734715358538725
33	25.846295437681427	23.41303566815041	23.903915293470856	26.836753600697307
34	26.384062200703045	23.372933682101728	24.572513698612262	25.670490418582965
35	26.150322425187177	23.18790781588578	24.16444771910168	26.49732203982536
36	25.78218445347778	23.101701594249672	24.67055044107835	26.445563511194198
37	25.335147346527265	22.61899894656171	25.08783726770642	26.958016439204606
38	25.667358810127972	22.361424151138383	24.992236220705326	26.97898081802832
39	24.870016501836776	22.57733115628553	25.91606071492881	26.63659162694888
40	25.756261694600326	21.84427381044548	26.19407795443336	26.205386540520838
41	24.89994076040673	22.19301320755866	26.33091184609188	26.576134185942728
42	24.25074092986154	22.269215679963533	26.89033890092722	26.589704489247712
43	23.87773156722215	21.769028218401854	26.720536131367496	27.632704083008502
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	14.0
1	14.5
2	15.0
3	34.0
4	53.0
5	53.0
6	72.0
7	91.0
8	92.5
9	94.0
10	120.5
11	147.0
12	147.0
13	267.5
14	388.0
15	596.5
16	805.0
17	760.5
18	716.0
19	716.0
20	1002.5
21	1289.0
22	1478.0
23	1667.0
24	2374.5
25	3082.0
26	3082.0
27	4116.5
28	5151.0
29	6567.5
30	7984.0
31	10549.0
32	13114.0
33	13114.0
34	16726.5
35	20339.0
36	24469.0
37	28599.0
38	34083.5
39	39568.0
40	39568.0
41	45560.5
42	51553.0
43	59150.5
44	66748.0
45	75979.0
46	85210.0
47	85210.0
48	94336.5
49	103463.0
50	110614.0
51	117765.0
52	120368.0
53	122971.0
54	122971.0
55	117594.5
56	112218.0
57	104911.0
58	97604.0
59	91028.0
60	84452.0
61	84452.0
62	74319.5
63	64187.0
64	54250.5
65	44314.0
66	37001.0
67	29688.0
68	29688.0
69	24190.5
70	18693.0
71	15066.5
72	11440.0
73	9198.0
74	6956.0
75	6956.0
76	5580.0
77	4204.0
78	3383.5
79	2563.0
80	1989.0
81	1415.0
82	1415.0
83	1006.0
84	597.0
85	430.5
86	264.0
87	181.0
88	98.0
89	98.0
90	67.5
91	37.0
92	23.5
93	10.0
94	6.0
95	2.0
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1149569.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	72.2384002315717
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.53361441119225	61.78811471088635
2	8.281662361045521	11.965080804398989
3	2.608284364851772	5.652548695977393
4	1.170194378522881	3.381318794578848
5	0.6760809344442625	2.4419502565659816
6	0.4167604819861027	1.8063666299048888
7	0.29834626311374135	1.508643973368297
8	0.20639393298252587	1.1927654028927914
9	0.15263454759067924	0.9923467984218328
>10	0.630904143892324	7.256160154397567
>50	0.01728375194555804	0.8581580679500079
>100	0.007478564025718647	1.0149513040320695
>500	3.6186440666807014E-4	0.14159440662492817
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	8.698912374985756E-5	8.698912374985756E-5
5	0.0	8.698912374985756E-5	0.0	1.7397824749971512E-4	8.698912374985756E-5
6	0.0	8.698912374985756E-5	0.0	1.7397824749971512E-4	8.698912374985756E-5
7	0.0	8.698912374985756E-5	0.0	1.7397824749971512E-4	8.698912374985756E-5
8	0.0	8.698912374985756E-5	0.0	1.7397824749971512E-4	1.7397824749971512E-4
9	0.0	8.698912374985756E-5	0.0	1.7397824749971512E-4	1.7397824749971512E-4
10	0.0	8.698912374985756E-5	0.0	3.4795649499943024E-4	1.7397824749971512E-4
11	0.0	8.698912374985756E-5	0.0	3.4795649499943024E-4	1.7397824749971512E-4
12	0.0	8.698912374985756E-5	0.0	3.4795649499943024E-4	1.7397824749971512E-4
13	0.0	8.698912374985756E-5	0.0	3.4795649499943024E-4	1.7397824749971512E-4
14	8.698912374985756E-5	8.698912374985756E-5	0.0	3.4795649499943024E-4	2.6096737124957267E-4
15	8.698912374985756E-5	8.698912374985756E-5	0.0	3.4795649499943024E-4	3.4795649499943024E-4
16	8.698912374985756E-5	8.698912374985756E-5	0.0	4.3494561874928776E-4	3.4795649499943024E-4
17	8.698912374985756E-5	8.698912374985756E-5	0.0	5.219347424991453E-4	3.4795649499943024E-4
18	8.698912374985756E-5	8.698912374985756E-5	0.0	6.959129899988605E-4	4.3494561874928776E-4
19	8.698912374985756E-5	8.698912374985756E-5	0.0	6.959129899988605E-4	5.219347424991453E-4
20	8.698912374985756E-5	8.698912374985756E-5	0.0	7.829021137487179E-4	5.219347424991453E-4
21	8.698912374985756E-5	8.698912374985756E-5	0.0	9.568803612484331E-4	5.219347424991453E-4
22	8.698912374985756E-5	8.698912374985756E-5	0.0	0.001391825979997721	5.219347424991453E-4
23	8.698912374985756E-5	8.698912374985756E-5	0.0	0.0015658042274974359	6.089238662490029E-4
24	8.698912374985756E-5	8.698912374985756E-5	0.0	0.0018267715987470086	6.089238662490029E-4
25	8.698912374985756E-5	8.698912374985756E-5	0.0	0.0022617172174962965	6.959129899988605E-4
26	8.698912374985756E-5	8.698912374985756E-5	0.0	0.003653543197494017	6.959129899988605E-4
27	8.698912374985756E-5	8.698912374985756E-5	0.0	0.011917509953730485	6.959129899988605E-4
28	8.698912374985756E-5	8.698912374985756E-5	0.0	0.04593025733992479	6.959129899988605E-4
29	8.698912374985756E-5	8.698912374985756E-5	0.0	0.0977757750948399	6.959129899988605E-4
30	8.698912374985756E-5	8.698912374985756E-5	0.0	0.16962879131222222	6.959129899988605E-4
31	8.698912374985756E-5	8.698912374985756E-5	0.0	0.5217607642516456	6.959129899988605E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATACG	50	2.7207898E-7	29.6	1
TTAGTAC	40	0.0019311877	23.125	3
TACATAT	95	7.1449904E-9	21.421053	35
CTTATAC	810	0.0	20.78395	37
TTACATA	105	2.2593667E-8	19.38095	34
TCGTTAC	185	0.0	18.0	23
TCTATAC	105	4.797257E-7	17.619047	3
CCTTATA	95	3.6063884E-6	17.526316	2
CTCGTTA	255	0.0	16.686274	22
GTATATA	90	4.445959E-5	16.444445	1
CGTTACG	215	0.0	16.348837	24
TCTTATA	1335	0.0	16.213484	37
TAGACCG	70	0.0025924942	15.857142	5
GCTTATA	140	3.4726327E-8	15.857142	1
GTGCGAT	70	0.0025924942	15.857142	11
ATAGACT	130	2.5887675E-7	15.653848	4
TACTTAC	225	0.0	15.622222	31
ATCTCGT	320	0.0	15.609375	20
TATACTG	145	5.3445547E-8	15.310345	5
TCTCGTT	285	0.0	14.929824	21
>>END_MODULE