FastQCFastQC Report
Thu 9 Feb 2017
SRR2727568.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727568.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1310537
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC7950.021.17610237
TATACCG701.21936304E-418.55
TACTTAC3200.017.92187531
GCTTATA2200.017.659091
CTATACT1054.7985304E-717.6190474
TATACTA650.00158009417.0769235
GTATAGA1201.04149876E-716.9583321
TATAGCA2850.016.8771934
CTACACG1005.8790556E-616.654
TGTTACG2350.016.53191624
GTTACGG5600.016.51785725
TCTTATA14200.016.41549337
TAGTACC803.3830185E-416.18754
GTACTTA3700.016.030
TACGGAT7750.015.75483827
CGACTTA2600.015.65384619
TACATAT2500.015.5435
AGTGTAC4650.015.51612927
CTCGTTA4850.015.25773222
TTATAGC2750.014.83
Produced by FastQC (version 0.11.5)