##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727568.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1310537
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.4818116543066	34.0	31.0	34.0	31.0	34.0
2	32.659857752966914	34.0	31.0	34.0	31.0	34.0
3	32.750796047727	34.0	31.0	34.0	31.0	34.0
4	36.23817946383811	37.0	37.0	37.0	35.0	37.0
5	36.079455215686394	37.0	35.0	37.0	35.0	37.0
6	36.100585485186606	37.0	35.0	37.0	35.0	37.0
7	36.077973380377664	37.0	35.0	37.0	35.0	37.0
8	36.06289177642447	37.0	35.0	37.0	35.0	37.0
9	37.765254243107975	39.0	37.0	39.0	35.0	39.0
10	37.725476655752566	39.0	37.0	39.0	35.0	39.0
11	37.80125017454677	39.0	37.0	39.0	35.0	39.0
12	37.769742479609505	39.0	37.0	39.0	35.0	39.0
13	37.806408365425774	39.0	37.0	39.0	35.0	39.0
14	39.14006929983663	40.0	39.0	41.0	36.0	41.0
15	39.149922512679915	40.0	39.0	41.0	36.0	41.0
16	39.13207105179022	40.0	38.0	41.0	36.0	41.0
17	39.14652237975731	40.0	39.0	41.0	36.0	41.0
18	39.114754486138125	40.0	38.0	41.0	36.0	41.0
19	39.146304148604735	40.0	39.0	41.0	36.0	41.0
20	39.12335630356106	40.0	39.0	41.0	36.0	41.0
21	39.102796792459884	40.0	39.0	41.0	36.0	41.0
22	39.070786250216514	40.0	39.0	41.0	36.0	41.0
23	39.02702708889562	40.0	39.0	41.0	36.0	41.0
24	38.9856577876092	40.0	38.0	41.0	36.0	41.0
25	38.92458816500412	40.0	38.0	41.0	35.0	41.0
26	38.817400805929175	40.0	38.0	41.0	35.0	41.0
27	38.769150355922804	40.0	38.0	41.0	35.0	41.0
28	38.70544822465905	40.0	38.0	41.0	35.0	41.0
29	38.62175199937125	40.0	38.0	41.0	35.0	41.0
30	38.567203367779776	40.0	38.0	41.0	35.0	41.0
31	38.49861469000875	40.0	38.0	41.0	34.0	41.0
32	38.408653857159315	40.0	38.0	41.0	34.0	41.0
33	38.36238503758383	40.0	38.0	41.0	34.0	41.0
34	38.32064413290124	40.0	38.0	41.0	34.0	41.0
35	38.30704817948673	40.0	38.0	41.0	34.0	41.0
36	38.25212565536112	40.0	38.0	41.0	34.0	41.0
37	38.22879170904751	40.0	38.0	41.0	34.0	41.0
38	38.19241043938477	40.0	38.0	41.0	34.0	41.0
39	38.146304148604735	40.0	38.0	41.0	34.0	41.0
40	38.09230872535457	40.0	38.0	41.0	33.0	41.0
41	38.041514280024145	40.0	38.0	41.0	33.0	41.0
42	38.00824165971659	40.0	37.0	41.0	33.0	41.0
43	36.901858551113015	39.0	36.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	0.0
12	4.0
13	0.0
14	0.0
15	2.0
16	2.0
17	8.0
18	21.0
19	41.0
20	82.0
21	145.0
22	328.0
23	579.0
24	979.0
25	1552.0
26	2535.0
27	3663.0
28	5389.0
29	7829.0
30	10917.0
31	15153.0
32	20399.0
33	28484.0
34	40627.0
35	58775.0
36	89966.0
37	153520.0
38	362372.0
39	507163.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.399003614548846	16.762060132602134	12.029648914910453	27.80928733793857
2	19.036776527484538	19.496816953660982	34.62572975810679	26.840676760747694
3	20.493507623210945	21.097763741122915	29.04969489606169	29.359033739604453
4	15.923014764176822	14.44323967961225	32.47371115809779	37.16003439811314
5	16.60830636601637	34.80123033535108	32.79663222022728	15.793831078405265
6	37.809005010923	32.129501112902574	14.462163220115112	15.59933065605931
7	30.9195390897014	29.27395411194037	20.028812616507587	19.77769418185065
8	28.126027727565113	33.8527641722439	18.913163077425512	19.108045022765477
9	28.806817358075353	13.665619513222444	17.310842807185146	40.21672032151706
10	17.16861103501847	25.84719088434741	32.33407374229038	24.650124338343748
11	38.24203360912359	20.331284046158178	19.788147911886504	21.638534432831733
12	22.754718104105418	22.96875250374465	28.961486779846734	25.315042612303202
13	32.49400818137909	18.626944527319715	22.51061969253825	26.36842759876295
14	23.74286265858957	18.88561711725804	23.778191687834834	33.59332853631756
15	27.24715135856523	25.951804489304763	20.591024900479727	26.21001925165028
16	26.059699192010605	24.729404816498885	23.071611102929563	26.139284888560947
17	25.87198987895801	24.201071774394773	23.57682385159671	26.3501144950505
18	25.88992145967645	24.41022267971068	24.111261261604973	25.588594599007884
19	27.096754994326755	24.044952565246156	24.020306179833153	24.83798626059394
20	26.153935371530906	23.422841171214547	24.025647501749283	26.397575955505264
21	25.806062705593202	24.3782510528127	24.51109735932675	25.304588882267343
22	26.664947269706996	23.430853154088744	23.360042486400612	26.544157089803644
23	25.99873181756791	23.7971152283377	24.040069070922833	26.164083883171557
24	25.69778647989336	23.501129689585262	24.12324108361687	26.677842746904513
25	26.305476304751412	23.091450298618046	23.685023772697757	26.91804962393278
26	25.92380070154448	23.723557595092696	24.06837807707833	26.284263626284492
27	26.708746109419266	23.924772822133217	23.54897267303403	25.817508395413487
28	26.007888369424137	24.514912589266842	22.994467153540874	26.482731887768146
29	25.960961041160992	24.44692519173438	24.051972588335925	25.540141178768707
30	26.989012900818523	23.666558059787704	23.288163554329255	26.056265485064518
31	26.172172170644554	23.701734479835366	24.096610778635018	26.029482570885065
32	25.67665010602524	23.836641010517063	23.914700615091373	26.572008268366325
33	25.65169850221703	23.473049597226176	24.020840312024767	26.854411588532027
34	26.139666411554956	23.681742674949277	24.70918409781639	25.469406815679374
35	26.04253065728018	23.341576773490562	24.225718159807773	26.39017440942148
36	25.732047244755396	23.165389454857056	24.7627499261753	26.33981337421225
37	25.024779918460904	22.924877359433577	25.377841297117136	26.672501424988383
38	25.74532424494692	22.30070574123432	25.188071759896896	26.765898253921865
39	24.602281354894977	22.81576178314691	26.228484964560327	26.353471897397785
40	25.78103479718619	21.920022097811813	26.29593822990118	26.003004875100817
41	24.702087770127818	22.525193870909405	26.406198375169872	26.3665199837929
42	24.152084221963975	22.486583743915663	26.92155963547767	26.439772398642692
43	23.780251912002484	22.11459882475657	26.575594584510014	27.529554678730932
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	20.0
1	26.0
2	32.0
3	78.0
4	124.0
5	124.0
6	143.0
7	162.0
8	161.5
9	161.0
10	239.0
11	317.0
12	317.0
13	492.5
14	668.0
15	974.5
16	1281.0
17	1251.5
18	1222.0
19	1222.0
20	1548.0
21	1874.0
22	2213.5
23	2553.0
24	3384.0
25	4215.0
26	4215.0
27	5438.0
28	6661.0
29	8427.0
30	10193.0
31	13467.0
32	16741.0
33	16741.0
34	21040.0
35	25339.0
36	29620.0
37	33901.0
38	39737.0
39	45573.0
40	45573.0
41	52414.5
42	59256.0
43	67480.5
44	75705.0
45	86042.5
46	96380.0
47	96380.0
48	107802.5
49	119225.0
50	127097.0
51	134969.0
52	136217.5
53	137466.0
54	137466.0
55	131073.5
56	124681.0
57	116768.0
58	108855.0
59	101608.0
60	94361.0
61	94361.0
62	82766.0
63	71171.0
64	60589.5
65	50008.0
66	41666.5
67	33325.0
68	33325.0
69	27210.0
70	21095.0
71	17256.5
72	13418.0
73	10857.5
74	8297.0
75	8297.0
76	6702.0
77	5107.0
78	4083.0
79	3059.0
80	2396.5
81	1734.0
82	1734.0
83	1262.5
84	791.0
85	580.5
86	370.0
87	265.0
88	160.0
89	160.0
90	103.5
91	47.0
92	29.5
93	12.0
94	8.0
95	4.0
96	4.0
97	3.0
98	2.0
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1310537.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	70.37051586526988
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.11153654259073	59.893427325878775
2	8.470517157471589	11.921493240337904
3	2.611711016888424	5.5136235464834105
4	1.2335319677861964	3.4721712363766444
5	0.70086775779215	2.466021283458431
6	0.4604670741036554	1.944198332618741
7	0.314958828983601	1.5514670690328123
8	0.2092979920602576	1.178272613667639
9	0.15398818192676964	0.9752605019407625
>10	0.6956053813326013	7.9627756511389505
>50	0.024391519435893412	1.1894281508540143
>100	0.012584170871457866	1.716966290157051
>500	5.42408756773083E-4	0.21489475805490355
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	7.63045988018652E-5
2	0.0	0.0	0.0	7.63045988018652E-5	7.63045988018652E-5
3	0.0	0.0	0.0	7.63045988018652E-5	7.63045988018652E-5
4	0.0	0.0	0.0	3.052183952074608E-4	1.526091976037304E-4
5	0.0	0.0	0.0	3.052183952074608E-4	1.526091976037304E-4
6	0.0	0.0	0.0	3.052183952074608E-4	2.2891379640559557E-4
7	0.0	0.0	0.0	3.052183952074608E-4	2.2891379640559557E-4
8	0.0	0.0	0.0	3.8152299400932594E-4	2.2891379640559557E-4
9	0.0	0.0	0.0	4.5782759281119115E-4	2.2891379640559557E-4
10	0.0	0.0	0.0	6.104367904149216E-4	2.2891379640559557E-4
11	0.0	0.0	0.0	6.104367904149216E-4	2.2891379640559557E-4
12	0.0	0.0	0.0	6.104367904149216E-4	2.2891379640559557E-4
13	0.0	0.0	0.0	6.104367904149216E-4	2.2891379640559557E-4
14	0.0	0.0	0.0	6.867413892167867E-4	2.2891379640559557E-4
15	0.0	0.0	0.0	8.393505868205171E-4	2.2891379640559557E-4
16	0.0	0.0	0.0	8.393505868205171E-4	3.8152299400932594E-4
17	0.0	0.0	0.0	9.156551856223823E-4	3.8152299400932594E-4
18	0.0	0.0	0.0	0.001144568982027978	5.341321916130564E-4
19	0.0	0.0	0.0	0.0012208735808298431	5.341321916130564E-4
20	0.0	0.0	0.0	0.0012971781796317081	6.104367904149216E-4
21	0.0	0.0	0.0	0.0016787011736410342	6.867413892167867E-4
22	0.0	0.0	0.0	0.0025943563592634162	6.867413892167867E-4
23	0.0	0.0	0.0	0.0030521839520746075	8.393505868205171E-4
24	0.0	0.0	0.0	0.004044143736498855	8.393505868205171E-4
25	0.0	0.0	0.0	0.0051124081197249675	8.393505868205171E-4
26	0.0	0.0	0.0	0.007401546083780923	8.393505868205171E-4
27	0.0	0.0	0.0	0.017550057724428993	8.393505868205171E-4
28	0.0	0.0	0.0	0.04860602943678813	8.393505868205171E-4
29	0.0	0.0	0.0	0.0984329324544061	8.393505868205171E-4
30	0.0	0.0	0.0	0.16382597362760457	8.393505868205171E-4
31	0.0	0.0	0.0	0.48781530014032415	8.393505868205171E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	795	0.0	21.176102	37
TATACCG	70	1.21936304E-4	18.5	5
TACTTAC	320	0.0	17.921875	31
GCTTATA	220	0.0	17.65909	1
CTATACT	105	4.7985304E-7	17.619047	4
TATACTA	65	0.001580094	17.076923	5
GTATAGA	120	1.04149876E-7	16.958332	1
TATAGCA	285	0.0	16.877193	4
CTACACG	100	5.8790556E-6	16.65	4
TGTTACG	235	0.0	16.531916	24
GTTACGG	560	0.0	16.517857	25
TCTTATA	1420	0.0	16.415493	37
TAGTACC	80	3.3830185E-4	16.1875	4
GTACTTA	370	0.0	16.0	30
TACGGAT	775	0.0	15.754838	27
CGACTTA	260	0.0	15.653846	19
TACATAT	250	0.0	15.54	35
AGTGTAC	465	0.0	15.516129	27
CTCGTTA	485	0.0	15.257732	22
TTATAGC	275	0.0	14.8	3
>>END_MODULE