##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727567.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1058072
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.617909745272534	34.0	31.0	34.0	31.0	34.0
2	32.791376201241505	34.0	31.0	34.0	31.0	34.0
3	32.88474697374092	34.0	31.0	34.0	31.0	34.0
4	36.3356151566245	37.0	37.0	37.0	35.0	37.0
5	36.1935038447289	37.0	35.0	37.0	35.0	37.0
6	36.21305166378092	37.0	36.0	37.0	35.0	37.0
7	36.1904955428364	37.0	36.0	37.0	35.0	37.0
8	36.17684524304584	37.0	36.0	37.0	35.0	37.0
9	37.9196226721811	39.0	38.0	39.0	35.0	39.0
10	37.88233882004249	39.0	38.0	39.0	35.0	39.0
11	37.96396180978232	39.0	38.0	39.0	35.0	39.0
12	37.92979778313763	39.0	38.0	39.0	35.0	39.0
13	37.96353650791251	39.0	38.0	39.0	35.0	39.0
14	39.31045240777565	40.0	39.0	41.0	36.0	41.0
15	39.33010702485275	40.0	39.0	41.0	36.0	41.0
16	39.30922753839058	40.0	39.0	41.0	36.0	41.0
17	39.32780566917941	40.0	39.0	41.0	36.0	41.0
18	39.297109270446626	40.0	39.0	41.0	36.0	41.0
19	39.33799968244127	40.0	39.0	41.0	36.0	41.0
20	39.30813971071912	40.0	39.0	41.0	36.0	41.0
21	39.28851061175421	40.0	39.0	41.0	36.0	41.0
22	39.27012339424916	40.0	39.0	41.0	36.0	41.0
23	39.22606779122782	40.0	39.0	41.0	36.0	41.0
24	39.18929052087193	40.0	39.0	41.0	36.0	41.0
25	39.126978126252276	40.0	39.0	41.0	36.0	41.0
26	39.04131760409499	40.0	39.0	41.0	36.0	41.0
27	39.000686153683304	40.0	39.0	41.0	36.0	41.0
28	38.919088682055666	40.0	38.0	41.0	35.0	41.0
29	38.843539948132076	40.0	38.0	41.0	35.0	41.0
30	38.791486779727656	40.0	38.0	41.0	35.0	41.0
31	38.7262785519322	40.0	38.0	41.0	35.0	41.0
32	38.63953492767978	40.0	38.0	41.0	35.0	41.0
33	38.59169697336287	40.0	38.0	41.0	35.0	41.0
34	38.54957413106102	40.0	38.0	41.0	35.0	41.0
35	38.54820749438601	40.0	38.0	41.0	35.0	41.0
36	38.47253589547781	40.0	38.0	41.0	34.0	41.0
37	38.47090746187405	40.0	38.0	41.0	34.0	41.0
38	38.4331935822893	40.0	38.0	41.0	34.0	41.0
39	38.38970126796664	40.0	38.0	41.0	34.0	41.0
40	38.33645158363514	40.0	38.0	41.0	34.0	41.0
41	38.28703717705412	40.0	38.0	41.0	34.0	41.0
42	38.253891039551185	40.0	38.0	41.0	34.0	41.0
43	37.14061330419858	39.0	36.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	1.0
17	6.0
18	8.0
19	15.0
20	50.0
21	99.0
22	198.0
23	341.0
24	576.0
25	975.0
26	1566.0
27	2331.0
28	3441.0
29	5054.0
30	7173.0
31	10080.0
32	14181.0
33	19790.0
34	29432.0
35	42988.0
36	66999.0
37	115208.0
38	276044.0
39	461515.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.05664264813737	15.613493221633309	11.468784732986034	28.861079397243287
2	19.24415351696293	19.008252746504965	34.011674063768815	27.73591967276329
3	20.47025155187927	20.516562199925904	28.93120694999962	30.08197929819521
4	16.19143120695	13.848206927316856	31.676955821531994	38.28340604420115
5	17.142595210911924	34.334619950249134	32.428322458207006	16.094462380631942
6	38.81853030795636	31.093158121564507	14.143838982602318	15.944472587876819
7	31.621761089982535	29.10841606242297	19.462097097361994	19.8077257502325
8	28.94699037494613	33.64525287504064	18.52690554139983	18.880851208613404
9	29.595717493705532	13.24304962233194	16.460883569360117	40.700349314602406
10	17.462989286173343	25.328994624184364	31.731394460868444	25.47662162877384
11	39.26405764447032	19.67172366341799	19.183099070762673	21.881119621349022
12	23.260515352452384	22.3798569473533	28.180974451644126	26.178653248550194
13	33.4328854747125	17.69992968342419	21.90900052170363	26.95818432015969
14	24.215365305952712	18.288736494302846	23.03652303434927	34.45937516539517
15	28.38294558404343	24.98090867162159	20.190308410013685	26.445837334321293
16	26.54923294444991	24.031918432772063	22.829164744932292	26.589683877845744
17	26.605750837372128	23.29898154378908	22.762250584081233	27.333017034757557
18	26.310307805139914	24.272828314141194	23.439614695408252	25.97724918531064
19	28.094590916308153	23.380450479740507	23.156647184690645	25.368311419260692
20	26.752054680588845	22.59836759691212	23.1212998737326	27.528277848766436
21	26.32731987993256	23.82730097762723	24.096375293930848	25.749003848509368
22	27.697737016006474	22.350747397152556	22.29659229239598	27.654923294444988
23	26.468236566131605	23.119409643200083	23.461541369585433	26.950812421082876
24	25.817335682259806	22.68805903567999	23.702451250954567	27.792154031105632
25	27.002604737673806	22.097551017322072	22.916304372481267	27.98353987252285
26	26.43383437043982	22.787579673216946	23.745264972516047	27.033320983827185
27	27.78241934386318	23.23622589010956	22.739378794637794	26.24197597138947
28	26.365786071269255	24.257895492934317	21.84076319947981	27.535555236316622
29	26.564260277183404	23.907068706099395	23.348222049161116	26.180448967556085
30	28.04185348445096	22.709702175277297	22.442045531873067	26.806398808398672
31	26.838438215924814	22.940593834824092	23.52533665005784	26.695631299193252
32	26.312859616358814	23.1033426836737	23.10787923695174	27.47591846301575
33	26.20700670653793	22.476258704511604	23.375157834249467	27.941576754701003
34	26.892026251521635	22.96667901617281	24.371309324885264	25.769985407420286
35	26.88862383656311	22.44261260103282	23.56711074482644	27.10165281757763
36	25.974508351038494	22.377683182240908	24.553716571273032	27.09409189544757
37	25.26255302096644	22.18242236823203	25.325781232279088	27.229243378522444
38	26.38449935354116	21.247608858376367	24.66438956895183	27.703502219130645
39	24.65276465117686	21.982152443311985	26.309268178347033	27.055814727164123
40	26.475891999788292	20.750761762904606	26.133098692716562	26.64024754459054
41	25.14753249306285	21.496741242561942	26.32486258024029	27.030863684134914
42	24.30789209051936	21.47500359143801	27.2147831149487	27.00232120309393
43	23.695457397984256	20.902641786192245	26.565205392449663	28.836695423373836
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	7.0
1	6.5
2	6.0
3	22.0
4	38.0
5	38.0
6	55.5
7	73.0
8	65.5
9	58.0
10	84.0
11	110.0
12	110.0
13	170.0
14	230.0
15	301.5
16	373.0
17	396.0
18	419.0
19	419.0
20	557.5
21	696.0
22	876.0
23	1056.0
24	1464.5
25	1873.0
26	1873.0
27	2452.0
28	3031.0
29	3898.0
30	4765.0
31	6378.0
32	7991.0
33	7991.0
34	10414.5
35	12838.0
36	16067.5
37	19297.0
38	23632.5
39	27968.0
40	27968.0
41	33304.0
42	38640.0
43	45775.0
44	52910.0
45	62911.5
46	72913.0
47	72913.0
48	84777.0
49	96641.0
50	107678.5
51	118716.0
52	121282.5
53	123849.0
54	123849.0
55	116681.0
56	109513.0
57	102058.5
58	94604.0
59	89302.0
60	84000.0
61	84000.0
62	73970.5
63	63941.0
64	54030.5
65	44120.0
66	36847.5
67	29575.0
68	29575.0
69	24110.5
70	18646.0
71	15322.5
72	11999.0
73	9703.0
74	7407.0
75	7407.0
76	6012.5
77	4618.0
78	3669.0
79	2720.0
80	2053.0
81	1386.0
82	1386.0
83	990.5
84	595.0
85	445.0
86	295.0
87	204.5
88	114.0
89	114.0
90	70.0
91	26.0
92	17.5
93	9.0
94	6.5
95	4.0
96	4.0
97	2.0
98	0.0
99	1.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1058072.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	69.91136565211382
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.79799422026908	59.98254946151178
2	7.967133216512745	11.139863269974484
3	2.545599053537406	5.338989187065852
4	1.2238981607327215	3.422575673437394
5	0.6830277065285547	2.3875699870821236
6	0.4124649823560951	1.730159412011378
7	0.27322360876539714	1.3371004932031436
8	0.2080975818250565	1.163870890743376
9	0.15208493553830446	0.9569218984736906
>10	0.6814939423176004	7.962126262308038
>50	0.03467910656050625	1.6728614046318053
>100	0.019355990005806796	2.4596123306213378
>500	9.474950507612198E-4	0.44579972893556974
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	9.451152662578728E-5	0.0
6	0.0	0.0	0.0	9.451152662578728E-5	0.0
7	0.0	0.0	0.0	9.451152662578728E-5	0.0
8	0.0	0.0	0.0	9.451152662578728E-5	0.0
9	0.0	0.0	0.0	9.451152662578728E-5	9.451152662578728E-5
10	0.0	0.0	0.0	9.451152662578728E-5	9.451152662578728E-5
11	0.0	0.0	0.0	9.451152662578728E-5	9.451152662578728E-5
12	0.0	0.0	0.0	9.451152662578728E-5	9.451152662578728E-5
13	0.0	0.0	0.0	9.451152662578728E-5	9.451152662578728E-5
14	0.0	0.0	0.0	2.8353457987736184E-4	3.780461065031491E-4
15	0.0	0.0	0.0	2.8353457987736184E-4	6.61580686380511E-4
16	0.0	0.0	0.0	2.8353457987736184E-4	7.560922130062982E-4
17	0.0	0.0	0.0	5.670691597547237E-4	7.560922130062982E-4
18	0.0	0.0	0.0	8.506037396320855E-4	8.506037396320855E-4
19	0.0	0.0	0.0	9.451152662578728E-4	8.506037396320855E-4
20	0.0	0.0	0.0	9.451152662578728E-4	8.506037396320855E-4
21	0.0	0.0	0.0	0.001323161372761022	8.506037396320855E-4
22	0.0	0.0	0.0	0.001701207479264171	8.506037396320855E-4
23	0.0	0.0	0.0	0.0024572996922704693	0.00103962679288366
24	0.0	0.0	0.0	0.003024368852025193	0.0011341383195094474
25	0.0	0.0	0.0	0.003780461065031491	0.0011341383195094474
26	0.0	0.0	0.0	0.006710318390430897	0.0011341383195094474
27	0.0	0.0	0.0	0.019185839905034818	0.0011341383195094474
28	0.0	0.0	0.0	0.06322821131265169	0.0011341383195094474
29	0.0	0.0	0.0	0.13174906811634746	0.0011341383195094474
30	0.0	0.0	0.0	0.2251264564226253	0.0011341383195094474
31	0.0	0.0	0.0	0.6011878208666329	0.0011341383195094474
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATACCG	35	8.8689296E-4	26.42857	5
ACTAGAC	50	9.090196E-6	25.900002	3
CCTTATA	55	5.142723E-4	20.181818	2
TACTTAC	265	0.0	19.54717	31
ACTTACA	215	0.0	18.069767	32
CTTATAC	655	0.0	17.793892	37
CATATAA	95	3.605839E-6	17.526316	37
GTACTTA	340	0.0	17.411764	30
TACACCG	75	2.0670707E-4	17.266666	5
TCTTATA	1190	0.0	17.10084	37
ACATATA	100	5.8769E-6	16.650002	36
TAGTACT	90	4.44542E-5	16.444445	4
CGTTACA	185	1.8189894E-11	16.000002	24
CTAGTAC	70	0.0025923115	15.857142	3
GTCTTAG	165	9.731593E-10	15.69697	1
GTGTACT	350	0.0	15.328572	28
TAAACTC	85	5.363652E-4	15.235293	5
TAGGACT	110	1.4515725E-5	15.136364	4
TTACATA	185	3.0377123E-10	15.000001	34
CCCTACG	75	0.0041041193	14.8	1
>>END_MODULE