##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727566.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3204145
Sequences flagged as poor quality	0
Sequence length	43
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.69278793562713	34.0	31.0	34.0	31.0	34.0
2	32.86397213609247	34.0	31.0	34.0	31.0	34.0
3	32.95443339798917	34.0	31.0	34.0	31.0	34.0
4	36.381923102730994	37.0	37.0	37.0	35.0	37.0
5	36.25451532312052	37.0	37.0	37.0	35.0	37.0
6	36.274566850126945	37.0	37.0	37.0	35.0	37.0
7	36.252133720540115	37.0	37.0	37.0	35.0	37.0
8	36.24028500582839	37.0	37.0	37.0	35.0	37.0
9	37.966622609151585	39.0	38.0	39.0	35.0	39.0
10	37.95489093034179	39.0	38.0	39.0	35.0	39.0
11	38.02247432622431	39.0	38.0	39.0	35.0	39.0
12	38.00265187748994	39.0	38.0	39.0	35.0	39.0
13	38.02783769149024	39.0	38.0	39.0	35.0	39.0
14	39.41824074753171	40.0	39.0	41.0	37.0	41.0
15	39.440825867743186	40.0	39.0	41.0	37.0	41.0
16	39.42719571055617	40.0	39.0	41.0	37.0	41.0
17	39.4431734518881	40.0	39.0	41.0	37.0	41.0
18	39.42140976766033	40.0	39.0	41.0	37.0	41.0
19	39.45791872714874	40.0	39.0	41.0	37.0	41.0
20	39.43627488768455	40.0	39.0	41.0	37.0	41.0
21	39.42032148982022	40.0	39.0	41.0	37.0	41.0
22	39.39300936755359	40.0	39.0	41.0	37.0	41.0
23	39.356887406780906	40.0	39.0	41.0	36.0	41.0
24	39.32647461335239	40.0	39.0	41.0	36.0	41.0
25	39.27117031220497	40.0	39.0	41.0	36.0	41.0
26	39.184295030343506	40.0	39.0	41.0	36.0	41.0
27	39.14639131499979	40.0	39.0	41.0	36.0	41.0
28	39.08963358399823	40.0	39.0	41.0	36.0	41.0
29	39.01949724497487	40.0	39.0	41.0	36.0	41.0
30	38.96599623300444	40.0	38.0	41.0	35.0	41.0
31	38.90132625084071	40.0	38.0	41.0	35.0	41.0
32	38.820662610462385	40.0	38.0	41.0	35.0	41.0
33	38.777418937033126	40.0	38.0	41.0	35.0	41.0
34	38.73799999687904	40.0	38.0	41.0	35.0	41.0
35	38.72746520522635	40.0	38.0	41.0	35.0	41.0
36	38.66645080044754	40.0	38.0	41.0	35.0	41.0
37	38.646367751771535	40.0	38.0	41.0	35.0	41.0
38	38.607239060654244	40.0	38.0	41.0	35.0	41.0
39	38.56718406938513	40.0	38.0	41.0	35.0	41.0
40	38.51547042970902	40.0	38.0	41.0	35.0	41.0
41	38.47252137465689	40.0	38.0	41.0	35.0	41.0
42	38.426476017783216	40.0	38.0	41.0	34.0	41.0
43	37.390016681517224	39.0	37.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	0.0
12	3.0
13	1.0
14	4.0
15	1.0
16	5.0
17	10.0
18	36.0
19	58.0
20	116.0
21	265.0
22	486.0
23	888.0
24	1540.0
25	2685.0
26	4213.0
27	6631.0
28	9795.0
29	14264.0
30	20320.0
31	28452.0
32	39457.0
33	54440.0
34	79508.0
35	117714.0
36	183245.0
37	319792.0
38	772356.0
39	1547859.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.239709813382355	17.014429746469027	12.14632920794783	27.599531232200793
2	18.330069332068305	19.65800548976404	35.92434175107556	26.087583427092092
3	19.98139909398607	21.521029791098716	29.43393635431605	29.06363476059916
4	15.329175177777534	14.668093984510689	33.427887938904135	36.57484289880764
5	16.06431668978776	35.29125554555115	33.27464893130617	15.36977883335492
6	36.75333045164935	32.93752311459063	14.962868409513302	15.346278024246718
7	30.38451755460505	29.849741506704596	20.497917541184936	19.267823397505417
8	27.40303575524828	34.74739751166068	19.279214891960258	18.570351841130787
9	28.456327663073928	14.22460594011819	17.959330804317535	39.35973559249035
10	16.941742648975	26.279834402001157	32.69730302467585	24.081119924347995
11	37.34259841548994	20.952734660884573	20.46124629191251	21.243420631712983
12	22.169814412269105	23.50146450925286	29.62075686337541	24.707964215102624
13	31.68399058095061	19.148758873271966	23.254596780108265	25.91265376566916
14	23.178476629490863	19.445281034410115	24.437439628980588	32.938802707118434
15	26.565183535701415	26.47155481415479	21.412170797513845	25.551090852629954
16	25.35902713516398	25.314928007315523	23.815214355155586	25.510830502364907
17	25.11069255604849	24.924621076761508	24.300866533817914	25.663819833372088
18	25.045589384999744	25.179540875959113	24.92290455019982	24.85196518884133
19	26.413661054665127	24.629971490054288	24.685493321931435	24.270874133349146
20	25.435958734701458	24.13692264238978	24.755621234369855	25.67149738853891
21	25.092965518102332	24.939632881782817	25.36439518186599	24.603006418248864
22	25.9432703576149	23.92897325183473	24.189479564751284	25.938276825799083
23	25.18444077905338	24.425236685605675	24.89934132194392	25.490981213397024
24	24.88320597226405	24.124251555407135	25.001490257151282	25.99105221517753
25	25.515824034180724	23.8564734117838	24.502043446847754	26.125659107187722
26	25.11755866229525	24.292034224418682	25.018031331291184	25.572375781994882
27	25.788033937290606	24.709368645925824	24.338723746896598	25.16387366988698
28	25.23378311530845	25.256690942513526	23.728482949429566	25.781042992748453
29	25.1646539092332	25.104388222131018	24.8195696511862	24.911388217449584
30	26.29915312821361	24.362380603874044	24.046508506949593	25.291957760962752
31	25.480900521043836	24.28594835751815	24.877650668118953	25.355500453319056
32	24.972683820488772	24.678315119946195	24.677191575287637	25.671809484277397
33	24.961042649443144	24.003095989725807	24.788953059240455	26.246908301590594
34	25.469540236162846	24.23089466924874	25.52877600732801	24.770789087260408
35	25.376722963536295	23.94869770250722	24.94172392323069	25.732855410725797
36	24.90508388353211	23.79605167681238	25.646248843295172	25.65261559636034
37	24.40847714444883	23.56185503465043	26.148566934392793	25.881100886507944
38	25.083945951260006	23.03160437495806	25.899264858487992	25.985184815293938
39	23.95281736625527	23.508673920811948	26.916540918092036	25.621967794840746
40	25.087628680974177	22.659242949367147	26.942569702681997	25.31055866697668
41	24.0089009704617	23.301411140881577	26.993659775072604	25.696028113584124
42	23.665595658124086	23.368636562952048	27.337995003347228	25.627772775576634
43	23.19386294939836	23.01471999550582	26.94356840904516	26.847848646050664
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	165.0
1	226.0
2	287.0
3	522.5
4	758.0
5	758.0
6	958.0
7	1158.0
8	1108.0
9	1058.0
10	1434.0
11	1810.0
12	1810.0
13	2940.0
14	4070.0
15	5332.0
16	6594.0
17	6561.5
18	6529.0
19	6529.0
20	8210.0
21	9891.0
22	10658.0
23	11425.0
24	14742.0
25	18059.0
26	18059.0
27	22444.0
28	26829.0
29	31969.5
30	37110.0
31	45321.0
32	53532.0
33	53532.0
34	64712.5
35	75893.0
36	87608.0
37	99323.0
38	113897.5
39	128472.0
40	128472.0
41	144441.5
42	160411.0
43	179077.5
44	197744.0
45	221613.0
46	245482.0
47	245482.0
48	269304.0
49	293126.0
50	308109.0
51	323092.0
52	321957.0
53	320822.0
54	320822.0
55	303036.5
56	285251.0
57	263771.5
58	242292.0
59	225076.5
60	207861.0
61	207861.0
62	181922.5
63	155984.0
64	131254.0
65	106524.0
66	88619.0
67	70714.0
68	70714.0
69	57766.5
70	44819.0
71	36401.5
72	27984.0
73	22621.5
74	17259.0
75	17259.0
76	13782.0
77	10305.0
78	8136.5
79	5968.0
80	4524.5
81	3081.0
82	3081.0
83	2277.0
84	1473.0
85	1061.5
86	650.0
87	437.0
88	224.0
89	224.0
90	149.5
91	75.0
92	48.5
93	22.0
94	16.0
95	10.0
96	10.0
97	7.5
98	5.0
99	4.5
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3204145.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.28912229745649
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.00418863727566	50.25964746293678
2	9.491070422113651	11.63398751669391
3	3.1810350868653656	5.848885454141745
4	1.5545948021545	3.8111900380894923
5	0.8956622353986563	2.7447176141280756
6	0.5762794159485523	2.1191795760946572
7	0.4124623672387695	1.7695619528156747
8	0.30368371857149745	1.4890006853819893
9	0.23983585875129335	1.3229396350491167
>10	1.2691945708267396	13.625885065255183
>50	0.049136940873778144	2.026865604660456
>100	0.021073468244065106	2.3373295216984427
>500	0.001324124834335702	0.5504144428193901
>1k	4.5835090312400234E-4	0.4603954302350778
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.120957384887388E-5
2	0.0	0.0	0.0	0.0	3.120957384887388E-5
3	0.0	0.0	0.0	3.120957384887388E-5	3.120957384887388E-5
4	0.0	0.0	0.0	3.120957384887388E-5	3.120957384887388E-5
5	3.120957384887388E-5	3.120957384887388E-5	0.0	3.120957384887388E-5	3.120957384887388E-5
6	3.120957384887388E-5	3.120957384887388E-5	0.0	3.120957384887388E-5	6.241914769774776E-5
7	3.120957384887388E-5	3.120957384887388E-5	0.0	3.120957384887388E-5	6.241914769774776E-5
8	3.120957384887388E-5	3.120957384887388E-5	0.0	6.241914769774776E-5	6.241914769774776E-5
9	3.120957384887388E-5	3.120957384887388E-5	0.0	1.8725744309324328E-4	9.362872154662164E-5
10	3.120957384887388E-5	3.120957384887388E-5	0.0	2.1846701694211716E-4	9.362872154662164E-5
11	3.120957384887388E-5	3.120957384887388E-5	0.0	2.1846701694211716E-4	9.362872154662164E-5
12	3.120957384887388E-5	3.120957384887388E-5	0.0	2.1846701694211716E-4	1.2483829539549553E-4
13	3.120957384887388E-5	3.120957384887388E-5	0.0	2.4967659079099105E-4	1.2483829539549553E-4
14	3.120957384887388E-5	3.120957384887388E-5	0.0	3.1209573848873883E-4	2.1846701694211716E-4
15	3.120957384887388E-5	3.120957384887388E-5	0.0	4.0572446003536044E-4	2.8088616463986494E-4
16	3.120957384887388E-5	3.120957384887388E-5	0.0	5.305627554308559E-4	2.8088616463986494E-4
17	3.120957384887388E-5	3.120957384887388E-5	0.0	6.866106246752253E-4	2.8088616463986494E-4
18	3.120957384887388E-5	3.120957384887388E-5	0.0	8.426584939195948E-4	3.4330531233761266E-4
19	3.120957384887388E-5	3.120957384887388E-5	0.0	9.674967893150903E-4	3.4330531233761266E-4
20	3.120957384887388E-5	3.120957384887388E-5	0.0	9.987063631639642E-4	3.7451488618648655E-4
21	3.120957384887388E-5	3.120957384887388E-5	0.0	0.0013732212493504507	3.7451488618648655E-4
22	3.120957384887388E-5	3.120957384887388E-5	0.0	0.0017165265616880634	3.7451488618648655E-4
23	3.120957384887388E-5	3.120957384887388E-5	0.0	0.002309508464816667	4.993531815819821E-4
24	6.241914769774776E-5	3.120957384887388E-5	0.0	0.0035891009926204962	4.993531815819821E-4
25	6.241914769774776E-5	3.120957384887388E-5	0.0	0.004494178634237839	5.305627554308559E-4
26	6.241914769774776E-5	3.120957384887388E-5	0.0	0.006928525394450002	5.617723292797299E-4
27	6.241914769774776E-5	3.120957384887388E-5	0.0	0.019443564507848426	5.617723292797299E-4
28	6.241914769774776E-5	3.120957384887388E-5	0.0	0.059329399886709246	5.617723292797299E-4
29	6.241914769774776E-5	3.120957384887388E-5	0.0	0.1151945370761935	5.617723292797299E-4
30	6.241914769774776E-5	6.241914769774776E-5	0.0	0.194997417407764	5.617723292797299E-4
31	6.241914769774776E-5	6.241914769774776E-5	0.0	0.5539699358175114	5.617723292797299E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	2130	0.0	20.758215	37
TACTTAC	905	0.0	17.78453	31
GTACTTA	1065	0.0	15.98122	30
TATTCCG	115	2.212173E-5	14.47826	5
TAAGACT	410	0.0	14.439025	4
TCTTATA	3400	0.0	14.419118	37
TATACTG	445	0.0	14.13483	5
ACTTACA	925	0.0	13.6	32
CTCGTTA	1200	0.0	13.4125	22
ATCTCGT	1315	0.0	13.365019	20
ACGGATG	1935	0.0	13.289406	28
TTTATAC	335	0.0	13.253732	3
TACGGAT	1885	0.0	13.249337	27
GGTATCA	3100	0.0	13.188709	1
AGTGTAC	1425	0.0	13.11228	27
GTTACGG	1510	0.0	13.109272	25
GTATACG	85	0.009409529	13.058824	1
TCGTTAC	1110	0.0	13.0	23
GTATTAT	315	0.0	12.920635	1
GTATAGG	375	0.0	12.826668	1
>>END_MODULE