FastQCFastQC Report
Thu 9 Feb 2017
SRR2727565.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727565.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1011905
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC6400.020.52343837
GTATATA701.2190145E-418.51
TACTTAC3400.016.86764531
TAATAGT1005.876391E-616.6500024
TTACATA1708.54925E-1116.32352834
GCGCTAT803.3820653E-416.18751
GTACTAG700.002592206515.8571421
GTACTTA3750.015.29333230
TTTATAC855.363365E-415.2352933
TATGACT1101.4514475E-515.1363644
TCTTATA10800.015.07407337
CTTACAT1853.0377123E-1015.033
GTTCTAA750.00410395414.81
GATAAAG1001.0931583E-414.82
TATTAGG750.00410395414.82
CGTACAC750.00410395414.83
CGTAACA1651.6438207E-814.57575714
TTATTGT1651.6438207E-814.5757574
GGATAGA1152.209216E-514.4782611
ACTTACA3200.014.45312532
Produced by FastQC (version 0.11.5)