##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727565.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1011905
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.645783942168485	34.0	31.0	34.0	31.0	34.0
2	32.81926465429067	34.0	31.0	34.0	31.0	34.0
3	32.90949644482436	34.0	31.0	34.0	31.0	34.0
4	36.35694358660151	37.0	37.0	37.0	35.0	37.0
5	36.21704409010727	37.0	35.0	37.0	35.0	37.0
6	36.23580474451653	37.0	36.0	37.0	35.0	37.0
7	36.21300912635079	37.0	36.0	37.0	35.0	37.0
8	36.20344498742471	37.0	36.0	37.0	35.0	37.0
9	37.93727474417065	39.0	38.0	39.0	35.0	39.0
10	37.912300067694105	39.0	38.0	39.0	35.0	39.0
11	37.99634451850717	39.0	38.0	39.0	35.0	39.0
12	37.9651953493658	39.0	38.0	39.0	35.0	39.0
13	38.00119181148428	39.0	38.0	39.0	35.0	39.0
14	39.352912575785275	40.0	39.0	41.0	37.0	41.0
15	39.37947633424086	40.0	39.0	41.0	37.0	41.0
16	39.358761939114835	40.0	39.0	41.0	36.0	41.0
17	39.37429205310775	40.0	39.0	41.0	37.0	41.0
18	39.34629337734273	40.0	39.0	41.0	36.0	41.0
19	39.380099910564724	40.0	39.0	41.0	37.0	41.0
20	39.35569445748366	40.0	39.0	41.0	37.0	41.0
21	39.336647214906534	40.0	39.0	41.0	36.0	41.0
22	39.31031865639561	40.0	39.0	41.0	36.0	41.0
23	39.27294459460127	40.0	39.0	41.0	36.0	41.0
24	39.236448085541625	40.0	39.0	41.0	36.0	41.0
25	39.17533562933279	40.0	39.0	41.0	36.0	41.0
26	39.09105597857506	40.0	39.0	41.0	36.0	41.0
27	39.05290911696256	40.0	39.0	41.0	36.0	41.0
28	38.97735162885844	40.0	38.0	41.0	36.0	41.0
29	38.90611569267866	40.0	38.0	41.0	35.0	41.0
30	38.850916835078394	40.0	38.0	41.0	35.0	41.0
31	38.783155533375165	40.0	38.0	41.0	35.0	41.0
32	38.70057959986362	40.0	38.0	41.0	35.0	41.0
33	38.66519386701321	40.0	38.0	41.0	35.0	41.0
34	38.62061063044456	40.0	38.0	41.0	35.0	41.0
35	38.6151239493826	40.0	38.0	41.0	35.0	41.0
36	38.5429956369422	40.0	38.0	41.0	35.0	41.0
37	38.53872843794625	40.0	38.0	41.0	35.0	41.0
38	38.502035270109346	40.0	38.0	41.0	35.0	41.0
39	38.46123895029672	40.0	38.0	41.0	34.0	41.0
40	38.414585361274035	40.0	38.0	41.0	34.0	41.0
41	38.36267040878343	40.0	38.0	41.0	34.0	41.0
42	38.32981258122057	40.0	38.0	41.0	34.0	41.0
43	37.23069754571822	39.0	36.0	40.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	1.0
12	1.0
13	0.0
14	0.0
15	0.0
16	1.0
17	4.0
18	7.0
19	19.0
20	37.0
21	75.0
22	142.0
23	309.0
24	554.0
25	860.0
26	1326.0
27	2066.0
28	3166.0
29	4524.0
30	6225.0
31	9112.0
32	12789.0
33	17945.0
34	26722.0
35	40394.0
36	63106.0
37	109732.0
38	261680.0
39	451106.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.793241460413775	16.110998562117985	11.793498401529789	28.302261575938452
2	19.32523309994515	19.221962536008817	34.16714019596701	27.28566416807902
3	20.688009249880178	20.549557517751172	28.94125436676368	29.821178865604974
4	16.261506761998408	14.15310725809241	31.767112525385286	37.81827345452389
5	17.092414801784752	34.327036628932554	32.45779000993176	16.122758559350927
6	38.46546859636033	31.318453807422635	14.238589590920094	15.977488005296939
7	31.394152613140562	29.095814330396628	19.510823644512083	19.999209411950726
8	28.509197997835766	33.475968593889746	18.78447087424215	19.230362534032345
9	29.22873194618072	13.50858035092227	16.861266620878443	40.401421082018565
10	17.54512528349993	25.576215158537607	31.89133367262737	24.987325885335085
11	38.70679559840104	19.927463546479167	19.376720146654083	21.989020708465716
12	23.15167925842841	22.639279378993088	28.250873352735685	25.95816800984282
13	33.050533399874496	18.05792045695989	22.110474797535343	26.78107134563027
14	24.09643197731012	18.69691324778512	23.21186277367935	33.99479200122541
15	28.120920442136367	25.266205819716276	20.379185793132752	26.2336879450146
16	26.46572553747634	24.185867250384177	22.871613441973306	26.476793770166175
17	26.26728793710872	23.621980324239924	23.1596839624273	26.95104777622405
18	26.153146787494876	24.168869607324797	23.83099203976658	25.846991565413752
19	27.738967590831155	23.687203838304978	23.438465073302336	25.13536349756153
20	26.575320805806868	22.92873342853331	23.49805564751632	26.997890118143502
21	26.250487941061664	23.91815437219897	24.28459193303719	25.54676575370218
22	27.398718259125115	22.777533464109773	22.640959378597795	27.18278889816732
23	26.442897307553576	23.372352147681845	23.561994456001305	26.62275608876327
24	25.856280974992714	22.944248719000303	23.773773229700417	27.42569707630657
25	26.772770171112903	22.422658253492177	23.096931036016226	27.707640539378698
26	26.224793829460275	23.082305157104667	23.854017916701668	26.83888309673339
27	27.26501005529175	23.48688859132033	23.14950514129291	26.09859621209501
28	26.34071380218499	24.40476131652675	22.21631477263182	27.038210108656447
29	26.31630439616367	24.107006092469156	23.694615601266918	25.882073910100257
30	27.684614662443607	23.16007925645194	22.704404069551984	26.45090201155247
31	26.542906695786662	23.24032394345319	23.748869706148305	26.46789965461185
32	26.076756217233832	23.398836847332507	23.513076820452515	27.011330114981146
33	26.105217387007674	22.813307573339394	23.595396801083105	27.486078238569828
34	26.58144786318874	23.167095725389242	24.428182487486474	25.823273923935545
35	26.54745257706998	22.764192290778283	23.86054026810817	26.82781486404356
36	25.806968045419282	22.543717048537165	24.78078475746241	26.868530148581144
37	25.131114086796686	22.305552398693553	25.423236371003206	27.14009714350655
38	26.029716228302064	21.476126711499596	25.1199470306007	27.37421002959764
39	24.676031840933685	22.11383479674475	26.41473260829821	26.79540075402335
40	26.136149144435493	21.031915051314108	26.44220554301046	26.38973026123994
41	24.82841768743113	21.703816069690337	26.635800791576287	26.83196545130225
42	24.20563195161601	21.761627820793453	27.20176301134988	26.830977216240655
43	23.609923856488503	21.357637327614746	26.703297246283004	28.329141569613746
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	7.0
1	15.0
2	23.0
3	43.0
4	63.0
5	63.0
6	67.0
7	71.0
8	82.5
9	94.0
10	118.0
11	142.0
12	142.0
13	227.5
14	313.0
15	459.0
16	605.0
17	604.5
18	604.0
19	604.0
20	775.0
21	946.0
22	1105.0
23	1264.0
24	1747.5
25	2231.0
26	2231.0
27	2966.5
28	3702.0
29	4716.5
30	5731.0
31	7545.5
32	9360.0
33	9360.0
34	12087.0
35	14814.0
36	17931.0
37	21048.0
38	25261.5
39	29475.0
40	29475.0
41	34598.0
42	39721.0
43	46649.0
44	53577.0
45	62447.5
46	71318.0
47	71318.0
48	81770.5
49	92223.0
50	101079.0
51	109935.0
52	112487.0
53	115039.0
54	115039.0
55	108597.5
56	102156.0
57	95622.5
58	89089.0
59	83220.5
60	77352.0
61	77352.0
62	68444.5
63	59537.0
64	50201.5
65	40866.0
66	34046.0
67	27226.0
68	27226.0
69	22338.5
70	17451.0
71	14072.0
72	10693.0
73	8636.0
74	6579.0
75	6579.0
76	5300.5
77	4022.0
78	3198.0
79	2374.0
80	1843.5
81	1313.0
82	1313.0
83	924.5
84	536.0
85	393.5
86	251.0
87	182.5
88	114.0
89	114.0
90	69.0
91	24.0
92	17.5
93	11.0
94	7.5
95	4.0
96	4.0
97	2.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1011905.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	72.63618816308639
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.45023630520855	62.794156310084105
2	7.826350011814977	11.369524641767319
3	2.394776718977388	5.218423571046606
4	1.1186939816004104	3.2503066617775884
5	0.6423610215113237	2.3329328013564443
6	0.40120798205195257	1.7485331086114688
7	0.26207435896239273	1.3325257715218823
8	0.18237875544254004	1.0597838077819084
9	0.13657337412300866	0.8928152370779805
>10	0.5432590816571196	6.4472557567642585
>50	0.02520644244657213	1.2655171805769871
>100	0.01647353201837943	2.0872799784302862
>500	4.0843418540071155E-4	0.20094517320327362
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	9.882350615917502E-5	9.882350615917502E-5
10	0.0	0.0	0.0	9.882350615917502E-5	9.882350615917502E-5
11	0.0	0.0	0.0	9.882350615917502E-5	9.882350615917502E-5
12	0.0	0.0	0.0	9.882350615917502E-5	9.882350615917502E-5
13	0.0	0.0	0.0	1.9764701231835005E-4	9.882350615917502E-5
14	0.0	0.0	0.0	1.9764701231835005E-4	2.964705184775251E-4
15	0.0	0.0	0.0	1.9764701231835005E-4	2.964705184775251E-4
16	0.0	0.0	0.0	1.9764701231835005E-4	2.964705184775251E-4
17	0.0	0.0	0.0	4.941175307958751E-4	2.964705184775251E-4
18	0.0	0.0	0.0	4.941175307958751E-4	2.964705184775251E-4
19	0.0	0.0	0.0	7.905880492734002E-4	2.964705184775251E-4
20	0.0	0.0	0.0	8.894115554325751E-4	2.964705184775251E-4
21	0.0	0.0	0.0	0.0012847055800692753	2.964705184775251E-4
22	0.0	0.0	0.0	0.0019764701231835004	2.964705184775251E-4
23	0.0	0.0	0.0	0.0027670581724569005	2.964705184775251E-4
24	0.0	0.0	9.882350615917502E-5	0.0033599992094119507	2.964705184775251E-4
25	0.0	0.0	9.882350615917502E-5	0.004348234271003701	2.964705184775251E-4
26	0.0	0.0	9.882350615917502E-5	0.006621174912664726	2.964705184775251E-4
27	0.0	0.0	9.882350615917502E-5	0.020159995256471705	2.964705184775251E-4
28	0.0	0.0	9.882350615917502E-5	0.06640939613896561	2.964705184775251E-4
29	0.0	0.0	9.882350615917502E-5	0.1381552616105267	2.964705184775251E-4
30	0.0	0.0	9.882350615917502E-5	0.22541641754907824	2.964705184775251E-4
31	0.0	0.0	9.882350615917502E-5	0.5920516253996175	2.964705184775251E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	640	0.0	20.523438	37
GTATATA	70	1.2190145E-4	18.5	1
TACTTAC	340	0.0	16.867645	31
TAATAGT	100	5.876391E-6	16.650002	4
TTACATA	170	8.54925E-11	16.323528	34
GCGCTAT	80	3.3820653E-4	16.1875	1
GTACTAG	70	0.0025922065	15.857142	1
GTACTTA	375	0.0	15.293332	30
TTTATAC	85	5.363365E-4	15.235293	3
TATGACT	110	1.4514475E-5	15.136364	4
TCTTATA	1080	0.0	15.074073	37
CTTACAT	185	3.0377123E-10	15.0	33
GTTCTAA	75	0.004103954	14.8	1
GATAAAG	100	1.0931583E-4	14.8	2
TATTAGG	75	0.004103954	14.8	2
CGTACAC	75	0.004103954	14.8	3
CGTAACA	165	1.6438207E-8	14.575757	14
TTATTGT	165	1.6438207E-8	14.575757	4
GGATAGA	115	2.209216E-5	14.478261	1
ACTTACA	320	0.0	14.453125	32
>>END_MODULE