FastQCFastQC Report
Thu 9 Feb 2017
SRR2727564.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727564.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2068646
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTTAC6050.020.48760431
CTTATAC12250.020.23673437
GTACTTA6700.018.530
ACCGTAT609.2402234E-418.58
TCTTATA21500.016.17674437
TACATAT3100.016.11290435
TCGTTAC8200.015.56707323
GGGTATA1101.4529054E-515.1363641
AGTGTAC8750.015.01142927
TACGGAT15200.014.72697427
ACTTACA5550.014.66666732
TTACATA3600.014.38888834
CTCGTTA9300.014.3225822
GTTACGG12150.014.16049325
TAAGACA2500.014.0599994
ATCGGTT800.006300866613.87511
ACGGATG16250.013.66153828
TAACCTA950.001245662613.6315795
CGTTACG7200.013.61805524
AACACCG1652.5977715E-713.4545455
Produced by FastQC (version 0.11.5)