FastQCFastQC Report
Thu 9 Feb 2017
SRR2727563.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727563.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1691611
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC12050.021.64730337
TACTTAC6300.019.08730331
CTATGCG609.239274E-418.59
GTACTTA6850.018.0948930
GGGTATA1054.8005677E-717.6190471
CGTTACG5900.016.30508424
TAATACA1504.6748028E-916.0333334
TCTTATA19950.015.30075237
ATCTCGT9800.015.29081720
ATTAGAC855.366009E-415.2352943
CGATCAA855.366009E-415.23529410
ACTTACA5500.015.13636432
TCGTTAC7300.014.95205523
CTCGTTA8800.014.92613622
GGGCGTC7950.014.66037711
CGACTTA1651.6463673E-814.57575719
CGTCAGA8050.014.47826114
GTATTAT908.2768605E-414.3888881
AGTGTAC9200.014.27717327
GCGTCAG8150.014.07361913
Produced by FastQC (version 0.11.5)