FastQCFastQC Report
Thu 9 Feb 2017
SRR2727562.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727562.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3313570
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT57070.1722311585389776No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT52520.15849974498803404No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT44810.13523178927863302No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22600.019.8097341
CTTATAC26750.018.18878637
TACTTAC10750.016.34883931
GTACTTA11700.015.49572730
TCTTATA44700.014.89932837
TATACCG1551.2122473E-714.3225815
AGTGTAC13750.013.99272727
GTATCAA32000.013.8750012
GTACTAT3000.013.5666671
TACACCG1951.0279109E-813.282055
TACGCGT850.00940961713.0588245
TCGTTAC6600.012.89393923
TAGTACT2753.6379788E-1212.7818174
GTATAGG4300.012.4767441
ACGGATG14400.012.46180528
TACGGAT13400.012.42537327
GTATTAG3150.012.3333331
TATACTG4200.012.3333335
ACTTACA11600.012.28017232
CTCTTAT67550.012.21465737
Produced by FastQC (version 0.11.5)