##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727562.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3313570
Sequences flagged as poor quality	0
Sequence length	43
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.722741333365526	34.0	31.0	34.0	31.0	34.0
2	32.8885030344915	34.0	31.0	34.0	31.0	34.0
3	32.97963555923068	34.0	31.0	34.0	31.0	34.0
4	36.39901405432811	37.0	37.0	37.0	35.0	37.0
5	36.277944030154785	37.0	37.0	37.0	35.0	37.0
6	36.29665557087974	37.0	37.0	37.0	35.0	37.0
7	36.27542831447653	37.0	37.0	37.0	35.0	37.0
8	36.26726672440902	37.0	37.0	37.0	35.0	37.0
9	37.98960728157244	39.0	38.0	39.0	35.0	39.0
10	37.99021780134417	39.0	38.0	39.0	35.0	39.0
11	38.05115630573671	39.0	38.0	39.0	35.0	39.0
12	38.03440549015111	39.0	38.0	39.0	35.0	39.0
13	38.05757355359929	39.0	38.0	39.0	35.0	39.0
14	39.46039498184737	40.0	39.0	41.0	37.0	41.0
15	39.480552998729465	40.0	39.0	41.0	37.0	41.0
16	39.47017386082081	40.0	39.0	41.0	37.0	41.0
17	39.48483056039257	40.0	39.0	41.0	37.0	41.0
18	39.463665774376274	40.0	39.0	41.0	37.0	41.0
19	39.50268079443018	41.0	39.0	41.0	37.0	41.0
20	39.478489967014426	41.0	39.0	41.0	37.0	41.0
21	39.461512205868594	41.0	39.0	41.0	37.0	41.0
22	39.429078607061264	40.0	39.0	41.0	37.0	41.0
23	39.392397021943104	40.0	39.0	41.0	37.0	41.0
24	39.35853716686233	41.0	39.0	41.0	36.0	41.0
25	39.30670666380973	40.0	39.0	41.0	36.0	41.0
26	39.21970925617989	40.0	39.0	41.0	36.0	41.0
27	39.18085508982759	40.0	39.0	41.0	36.0	41.0
28	39.12765627404884	40.0	39.0	41.0	36.0	41.0
29	39.057420244630414	40.0	39.0	41.0	36.0	41.0
30	38.99243293487085	40.0	39.0	41.0	35.0	41.0
31	38.92473646248608	40.0	38.0	41.0	35.0	41.0
32	38.84216479506997	40.0	38.0	41.0	35.0	41.0
33	38.79512489550545	40.0	38.0	41.0	35.0	41.0
34	38.75050051756867	40.0	38.0	41.0	35.0	41.0
35	38.72933271365929	40.0	38.0	41.0	35.0	41.0
36	38.67493368179939	40.0	38.0	41.0	35.0	41.0
37	38.64934375914799	40.0	38.0	41.0	35.0	41.0
38	38.600352489912694	40.0	38.0	41.0	35.0	41.0
39	38.56074988607454	40.0	38.0	41.0	35.0	41.0
40	38.501121147282234	40.0	38.0	41.0	35.0	41.0
41	38.453858527207814	40.0	38.0	41.0	35.0	41.0
42	38.404812935896935	40.0	38.0	41.0	34.0	41.0
43	37.387627543706635	39.0	37.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	2.0
10	6.0
11	0.0
12	0.0
13	3.0
14	1.0
15	1.0
16	11.0
17	12.0
18	16.0
19	52.0
20	112.0
21	265.0
22	502.0
23	912.0
24	1562.0
25	2764.0
26	4239.0
27	6573.0
28	9905.0
29	14639.0
30	20687.0
31	29035.0
32	39930.0
33	55081.0
34	80580.0
35	119368.0
36	186056.0
37	329667.0
38	783609.0
39	1627979.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.07761719233334	17.414933138578633	12.362436888310794	27.14501278077723
2	17.982689365246546	19.804863032922196	36.34023123096841	25.872216370862844
3	19.645759709316536	21.92064751914099	29.597714851353672	28.8358779201888
4	14.906641477319024	14.94186028965738	34.036914868253874	36.11458336476972
5	15.674785805038072	35.450978853623134	33.73600074843748	15.138234592901313
6	36.04722399104289	33.713245834553064	15.212323868214645	15.027206306189397
7	30.005039881457158	30.040922630274903	20.973300699849407	18.980736788418533
8	27.02001164906732	35.061670645255724	19.530446014419493	18.387871691257466
9	27.845073440428298	14.506227422387335	18.526061015762455	39.12263812142191
10	16.701412675754547	26.440877965457194	33.155509012937706	23.70220034585055
11	36.6905784395682	21.265764719019064	21.154464821929217	20.88919201948352
12	21.86714027468863	23.878143512887913	30.248432959013993	24.006283253409464
13	31.104397975597315	19.526764184851988	23.89733731292835	25.471500526622343
14	22.80890399176719	19.65490392537354	25.198773528248985	32.33741855461029
15	25.756963033827564	26.901076482464532	22.053042488916788	25.288917994791117
16	24.947594286524804	25.6513065968125	24.250611877823616	25.150487238839077
17	24.579049182603658	25.420015270539025	24.97671695482516	25.02421859203216
18	24.656126172074227	25.359053830159013	25.52410240314827	24.46071759461849
19	25.761520052390622	24.9352511037944	25.466762434473996	23.836466409340986
20	24.925292056603602	24.57690647851109	25.570366704189134	24.92743476069617
21	24.67055170103544	25.185072293628924	25.99619141892279	24.148184586412842
22	25.398527871751615	24.313776380157957	25.131172723075114	25.156523025015314
23	24.7185965589983	24.790905277389644	25.577820900116794	24.912677263495265
24	24.5377342262273	24.527201779349763	25.686193440911165	25.24887055351177
25	25.011573619992937	24.408870191364603	25.305938911808113	25.273617276834347
26	24.639648475813093	24.734832823812383	25.666456420114862	24.95906228025966
27	25.100390213576297	25.219596990557015	25.07875192013448	24.601260875732216
28	24.782304282088504	25.402571848489696	24.701575641981307	25.113548227440496
29	24.647826966081897	25.393095664192998	25.564511991598188	24.394565378126913
30	25.526033854724663	24.787676131785354	24.909508475752737	24.776781537737243
31	24.82745196268677	24.67308673122946	25.576613742881545	24.92284756320223
32	24.3557552730137	25.05038372510616	25.50825846443564	25.085602537444508
33	24.468654653440247	24.419553532896543	25.62314965430035	25.48864215936286
34	24.82718035230884	24.547904525934264	26.22211089549942	24.40280422625748
35	24.7413816518136	24.33511288429096	25.76212363100825	25.161381832887187
36	24.41879905962451	24.126365219385736	26.312074288456255	25.14276143253349
37	24.00190730843169	23.810452170921394	26.65892074107383	25.528719779573088
38	24.31030580310662	23.47736731078565	26.717920550946562	25.49440633516117
39	23.59382780505618	23.71306476096778	27.563534194237633	25.129573239738406
40	24.3113017078257	23.259777219132236	27.536342977513677	24.892578095528386
41	23.42331684557743	23.596211940595794	27.74409473769982	25.23637647612696
42	23.07149086936446	23.89957055381356	27.806625482485657	25.222313094336318
43	22.686256816666013	23.514940079732735	27.648336990013796	26.150466113587463
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	901.0
1	928.0
2	955.0
3	1654.5
4	2354.0
5	2354.0
6	2720.0
7	3086.0
8	2885.0
9	2684.0
10	3573.0
11	4462.0
12	4462.0
13	6651.0
14	8840.0
15	11182.0
16	13524.0
17	13075.5
18	12627.0
19	12627.0
20	14813.5
21	17000.0
22	17080.5
23	17161.0
24	21175.5
25	25190.0
26	25190.0
27	30493.5
28	35797.0
29	41600.5
30	47404.0
31	55977.0
32	64550.0
33	64550.0
34	75878.5
35	87207.0
36	99345.0
37	111483.0
38	127003.0
39	142523.0
40	142523.0
41	158757.5
42	174992.0
43	193318.0
44	211644.0
45	233623.0
46	255602.0
47	255602.0
48	274883.0
49	294164.0
50	304726.0
51	315288.0
52	311934.5
53	308581.0
54	308581.0
55	293766.5
56	278952.0
57	259186.5
58	239421.0
59	221239.0
60	203057.0
61	203057.0
62	177825.0
63	152593.0
64	128328.5
65	104064.0
66	86716.0
67	69368.0
68	69368.0
69	56540.0
70	43712.0
71	35161.0
72	26610.0
73	21528.0
74	16446.0
75	16446.0
76	13213.0
77	9980.0
78	7838.0
79	5696.0
80	4460.5
81	3225.0
82	3225.0
83	2317.0
84	1409.0
85	1031.5
86	654.0
87	444.5
88	235.0
89	235.0
90	158.0
91	81.0
92	54.0
93	27.0
94	19.0
95	11.0
96	11.0
97	9.0
98	7.0
99	5.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3313570.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.7577672985844
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.17459146905371	49.31986939034419
2	9.874643776588004	11.99922617468697
3	3.359724616867417	6.123880993768684
4	1.6321376707331012	3.966601631906213
5	0.9322974134781281	2.8322154650588116
6	0.6119149534152772	2.230715180767772
7	0.4346492584605993	1.848582295143595
8	0.30910518715563434	1.5024432825589926
9	0.25824183513945104	1.4121177593545944
>10	1.346033748213349	14.239361704134845
>50	0.05165936511876036	2.129049684629811
>100	0.014056712238659026	1.505606653922164
>500	6.458903161618929E-4	0.2826692923923434
>1k	1.9873548118548709E-4	0.2768432372576321
>5k	9.936774059274354E-5	0.33081725407331697
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	5707	0.1722311585389776	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	5252	0.15849974498803404	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4481	0.13523178927863302	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	6.03578617623892E-5
7	0.0	0.0	0.0	3.01789308811946E-5	9.053679264358381E-5
8	0.0	0.0	0.0	3.01789308811946E-5	1.207157235247784E-4
9	0.0	0.0	0.0	9.053679264358381E-5	1.50894654405973E-4
10	0.0	0.0	0.0	1.207157235247784E-4	1.50894654405973E-4
11	0.0	0.0	0.0	1.50894654405973E-4	1.50894654405973E-4
12	0.0	0.0	0.0	1.50894654405973E-4	1.50894654405973E-4
13	0.0	0.0	0.0	1.8107358528716763E-4	1.50894654405973E-4
14	0.0	0.0	0.0	1.8107358528716763E-4	1.50894654405973E-4
15	0.0	3.01789308811946E-5	0.0	1.8107358528716763E-4	1.50894654405973E-4
16	0.0	3.01789308811946E-5	0.0	2.1125251616836222E-4	2.1125251616836222E-4
17	0.0	3.01789308811946E-5	0.0	3.3196823969314064E-4	2.1125251616836222E-4
18	0.0	3.01789308811946E-5	0.0	5.130418249803082E-4	2.1125251616836222E-4
19	0.0	3.01789308811946E-5	0.0	6.639364793862813E-4	2.1125251616836222E-4
20	0.0	3.01789308811946E-5	0.0	8.450100646734489E-4	2.1125251616836222E-4
21	0.0	3.01789308811946E-5	0.0	0.0012373361661289788	2.1125251616836222E-4
22	0.0	3.01789308811946E-5	0.0	0.0018710937146340654	2.1125251616836222E-4
23	0.0	3.01789308811946E-5	0.0	0.002655745917545125	2.716103779307514E-4
24	0.0	3.01789308811946E-5	0.0	0.003923261014555299	3.01789308811946E-4
25	0.0	3.01789308811946E-5	0.0	0.0053718496968526395	3.01789308811946E-4
26	0.0	3.01789308811946E-5	0.0	0.008993321402595992	3.01789308811946E-4
27	0.0	3.01789308811946E-5	0.0	0.02713085886219395	3.01789308811946E-4
28	0.0	3.01789308811946E-5	0.0	0.08311277564680994	3.01789308811946E-4
29	0.0	3.01789308811946E-5	0.0	0.16206085883201501	3.01789308811946E-4
30	0.0	3.01789308811946E-5	0.0	0.26569530747803727	3.01789308811946E-4
31	0.0	3.01789308811946E-5	0.0	0.703289805255359	3.01789308811946E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2260	0.0	19.809734	1
CTTATAC	2675	0.0	18.188786	37
TACTTAC	1075	0.0	16.348839	31
GTACTTA	1170	0.0	15.495727	30
TCTTATA	4470	0.0	14.899328	37
TATACCG	155	1.2122473E-7	14.322581	5
AGTGTAC	1375	0.0	13.992727	27
GTATCAA	3200	0.0	13.875001	2
GTACTAT	300	0.0	13.566667	1
TACACCG	195	1.0279109E-8	13.28205	5
TACGCGT	85	0.009409617	13.058824	5
TCGTTAC	660	0.0	12.893939	23
TAGTACT	275	3.6379788E-12	12.781817	4
GTATAGG	430	0.0	12.476744	1
ACGGATG	1440	0.0	12.461805	28
TACGGAT	1340	0.0	12.425373	27
GTATTAG	315	0.0	12.333333	1
TATACTG	420	0.0	12.333333	5
ACTTACA	1160	0.0	12.280172	32
CTCTTAT	6755	0.0	12.214657	37
>>END_MODULE