FastQCFastQC Report
Thu 9 Feb 2017
SRR2727561.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727561.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1083916
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTG12800.11809033172312247No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCGT701.9227082E-723.7857136
CTTATAC7900.021.31012537
TAGTACT759.264977E-619.7333324
TACTTAC2300.017.69565231
GTAGTAC1005.8771675E-616.6500023
GTATAGG904.4455795E-516.4444451
TATAAGA904.4455795E-516.4444452
TCTTATA13450.016.36802937
ATAGGAC1251.6573358E-716.2799993
TGTACCG1251.6573358E-716.2799995
GTACTTA2700.015.75925930
TACACCG957.0590824E-515.5789485
GTATAAG1201.9351555E-615.4166661
TTATTAC1201.9351555E-615.4166663
TAAGAGT1201.9351555E-615.4166664
GTGTAGG1455.343645E-815.3103451
ATCGCCC855.363802E-415.23529311
GTATTAA1001.09327215E-414.81
CTAAGGT1001.09327215E-414.84
TATACTG1001.09327215E-414.85
Produced by FastQC (version 0.11.5)