FastQCFastQC Report
Thu 9 Feb 2017
SRR2727560.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727560.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences846146
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTAGGT451.3228091E-424.6666663
GTCTTAC701.9216714E-723.7857131
TAATATA502.7012863E-422.1999994
CTTATAC4250.019.1529437
ACTAGAC609.234068E-418.53
AATATAG500.007033386718.4999985
CGTAGTC500.007033386718.49999814
TAATCAG650.001579468617.0769235
CTAGAAC650.001579468617.0769233
GTATTAT650.001579468617.0769231
GTATTAG650.001579468617.0769231
TACTTAC1655.4569682E-1116.81818231
GTTCTAG1005.8741043E-616.651
TCGTTAC2600.015.65384623
ATAGGAC1201.9339332E-615.4166673
ACTAATG750.004103215414.83
CTACACT2251.8189894E-1214.84
AGCCTAC750.004103215414.81
ATTATGC750.004103215414.83
CGTTACG2400.014.64583424
Produced by FastQC (version 0.11.5)