##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727560.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	846146
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.482746476376416	31.0	31.0	34.0	30.0	34.0
2	31.67605590524567	31.0	31.0	34.0	30.0	34.0
3	31.76629919659255	31.0	31.0	34.0	30.0	34.0
4	35.54836044843325	37.0	35.0	37.0	33.0	37.0
5	34.88554930236626	37.0	35.0	37.0	32.0	37.0
6	34.84513901856181	36.0	35.0	37.0	32.0	37.0
7	34.82228717029921	35.0	35.0	37.0	32.0	37.0
8	34.752517886984045	35.0	35.0	37.0	32.0	37.0
9	36.38497138791651	38.0	35.0	39.0	32.0	39.0
10	36.14183604247967	37.0	35.0	39.0	32.0	39.0
11	36.30230362136085	38.0	35.0	39.0	32.0	39.0
12	36.225440999543814	37.0	35.0	39.0	32.0	39.0
13	36.33111543397948	38.0	35.0	39.0	32.0	39.0
14	37.36590139290382	39.0	36.0	40.0	32.0	41.0
15	37.318003039664546	39.0	36.0	40.0	32.0	41.0
16	37.28481136825087	39.0	36.0	40.0	32.0	41.0
17	37.27560019192905	39.0	36.0	40.0	32.0	41.0
18	37.21883457464787	39.0	36.0	40.0	32.0	41.0
19	37.26898667605827	39.0	36.0	40.0	32.0	41.0
20	37.22913067012076	39.0	36.0	40.0	32.0	41.0
21	37.18027858076502	39.0	36.0	40.0	32.0	41.0
22	37.16710591316392	39.0	36.0	40.0	32.0	41.0
23	37.085117698364115	39.0	36.0	40.0	31.0	41.0
24	37.005450595996436	39.0	36.0	40.0	31.0	41.0
25	36.906355404386474	38.0	36.0	40.0	31.0	41.0
26	36.71760547234165	38.0	36.0	40.0	31.0	41.0
27	36.61945456221503	38.0	36.0	40.0	30.0	41.0
28	36.48971335916024	38.0	35.0	40.0	30.0	41.0
29	36.357079038369264	38.0	35.0	40.0	30.0	41.0
30	36.27167651918227	38.0	35.0	40.0	30.0	41.0
31	36.17181195680178	38.0	35.0	40.0	30.0	41.0
32	36.03148392830552	38.0	35.0	40.0	29.0	41.0
33	35.959116984539314	38.0	35.0	40.0	29.0	41.0
34	35.884339109326284	38.0	35.0	40.0	29.0	41.0
35	35.87892869552063	38.0	35.0	40.0	29.0	41.0
36	35.793308719771765	38.0	34.0	40.0	29.0	41.0
37	35.760289595412615	38.0	34.0	40.0	29.0	41.0
38	35.72176905640398	38.0	34.0	40.0	29.0	41.0
39	35.648285284099906	38.0	34.0	40.0	28.0	41.0
40	35.57530024369317	38.0	34.0	40.0	28.0	41.0
41	35.51534959687808	38.0	34.0	40.0	28.0	41.0
42	35.47170819220324	38.0	34.0	40.0	28.0	41.0
43	34.24946522231388	37.0	32.0	39.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	0.0
13	1.0
14	5.0
15	9.0
16	18.0
17	27.0
18	70.0
19	144.0
20	285.0
21	548.0
22	871.0
23	1521.0
24	2455.0
25	3708.0
26	5533.0
27	7915.0
28	10958.0
29	14807.0
30	20004.0
31	26386.0
32	34797.0
33	46329.0
34	63910.0
35	88699.0
36	123371.0
37	162433.0
38	170509.0
39	60832.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.93207082465674	16.9572390580349	12.076639256109466	28.034050861198896
2	19.584208871754992	19.743519439907534	33.805631652220775	26.866640036116703
3	20.784947278602036	21.13193231428146	28.844431102906594	29.238689304209913
4	16.55281712612244	14.613080957659788	32.09339759332314	36.74070432289463
5	16.710473133478146	34.650875853576096	32.806749662587784	15.831901350357978
6	38.20309970146996	32.181089315555475	14.210904501114467	15.404906481860102
7	31.484164671345134	29.161161312586714	19.586927078778366	19.767746937289782
8	28.640447393239466	33.99436976597419	18.552117483271207	18.813065357515132
9	29.06094220146405	13.418488062343851	16.766964566398705	40.753605169793396
10	17.234141625676894	25.88808550770198	32.05226993923034	24.82550292739078
11	39.167590463111566	19.931312090348474	19.461416824046914	21.439680622493047
12	23.103341503712123	22.89333046542795	28.589037825623475	25.41429020523645
13	32.939350892162814	18.198750570232562	22.28374299470777	26.57815554289685
14	23.910767172568328	18.479907722780702	23.47597223174251	34.13335287290845
15	27.52680979405445	25.475508954719402	20.53026309880328	26.467418152422866
16	26.403126647174364	24.3759351223075	22.838729959132348	26.38220827138579
17	26.23057959264713	23.845293838179227	23.271515790419148	26.652610778754493
18	26.250670688037285	24.260352232357064	23.694728805667108	25.794248273938543
19	27.7982759476497	23.527736348100685	23.50575432608557	25.16823337816405
20	26.663601789762048	23.122723501617923	23.597818816138112	26.61585589248191
21	26.46245447003236	23.75039295818925	24.157060365468844	25.630092206309552
22	27.165051894117564	23.010095184518985	22.95041281291881	26.87444010844464
23	26.461154457977702	23.353298366948493	23.741883788376946	26.44366338669686
24	26.065123512963485	23.071904848572235	23.874130469209806	26.988841169254478
25	26.759448133064506	22.65885556393341	23.409789799869056	27.17190650313303
26	26.37830823522182	23.1539237909297	23.803338903688015	26.664429070160466
27	27.217170559217912	23.432480919368526	23.19505144502249	26.155297076391072
28	26.402299366775946	24.357734953542295	22.53239984588948	26.70756583379228
29	26.466590872024447	24.04100474386217	23.55231839422511	25.940085989888267
30	27.45779097224356	23.446190137399455	22.84404819026504	26.25197070009195
31	26.724820539245	23.351761989065718	23.657264821910168	26.266152649779116
32	26.27726184370073	23.494763315078014	23.55196384548293	26.676010995738324
33	26.216988557530264	23.00678606292531	23.639064653144963	27.13716072639946
34	26.587964724763815	23.331079979105258	24.385744304174455	25.69521099195647
35	26.57094638513921	22.879030332826723	23.955203948254795	26.59481933377928
36	26.083323681728686	22.868984785131644	24.442472102923134	26.605219430216536
37	25.59688280745876	22.643019053449404	25.028541173745428	26.731556965346403
38	26.151987954797402	22.150669033476493	24.697274465635953	27.000068546090155
39	24.974295216192004	22.676583001042374	25.86066707164012	26.488454711125502
40	26.258116211623054	21.67699191392502	25.852276084741877	26.212615789710046
41	25.09815091012662	22.052577214806902	26.150215211086504	26.699056663979974
42	24.606864536380247	22.046549886189855	26.546955253585082	26.79963032384482
43	24.26767957302877	21.57440914452116	26.274661819591415	27.883249462858657
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	11.0
1	10.5
2	10.0
3	27.0
4	44.0
5	44.0
6	56.5
7	69.0
8	57.5
9	46.0
10	82.0
11	118.0
12	118.0
13	191.0
14	264.0
15	376.0
16	488.0
17	505.5
18	523.0
19	523.0
20	654.5
21	786.0
22	970.0
23	1154.0
24	1643.5
25	2133.0
26	2133.0
27	2725.0
28	3317.0
29	4239.5
30	5162.0
31	6838.5
32	8515.0
33	8515.0
34	10643.0
35	12771.0
36	15628.5
37	18486.0
38	22252.0
39	26018.0
40	26018.0
41	30659.5
42	35301.0
43	41055.0
44	46809.0
45	54100.5
46	61392.0
47	61392.0
48	69224.5
49	77057.0
50	83169.0
51	89281.0
52	91009.5
53	92738.0
54	92738.0
55	88845.0
56	84952.0
57	79553.0
58	74154.0
59	69316.0
60	64478.0
61	64478.0
62	56802.0
63	49126.0
64	41204.5
65	33283.0
66	27887.5
67	22492.0
68	22492.0
69	18323.5
70	14155.0
71	11467.5
72	8780.0
73	7029.0
74	5278.0
75	5278.0
76	4228.0
77	3178.0
78	2553.0
79	1928.0
80	1475.5
81	1023.0
82	1023.0
83	748.5
84	474.0
85	349.0
86	224.0
87	153.0
88	82.0
89	82.0
90	55.0
91	28.0
92	21.5
93	15.0
94	8.5
95	2.0
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	846146.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	76.54567101473636
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.92002822055915	67.29897555777258
2	7.118555050209119	10.897891459471948
3	2.1362886445086335	4.905709433252248
4	0.98524192905381	3.0166401828510865
5	0.5540722659857891	2.120591669526886
6	0.33145499660303085	1.5222867075699695
7	0.23028772776356476	1.23392700536847
8	0.14522008447364193	0.8892775048681295
9	0.10857566829561881	0.747989764700542
>10	0.4450658062232598	5.428986180909998
>50	0.01747777977473239	0.9185797841744501
>100	0.007731826549616057	1.0191447495337491
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	1.1818291405974856E-4	0.0
4	0.0	0.0	0.0	1.1818291405974856E-4	1.1818291405974856E-4
5	0.0	0.0	0.0	1.1818291405974856E-4	1.1818291405974856E-4
6	0.0	0.0	0.0	1.1818291405974856E-4	1.1818291405974856E-4
7	0.0	0.0	0.0	1.1818291405974856E-4	1.1818291405974856E-4
8	0.0	0.0	0.0	1.1818291405974856E-4	1.1818291405974856E-4
9	0.0	0.0	0.0	1.1818291405974856E-4	2.3636582811949712E-4
10	0.0	0.0	0.0	1.1818291405974856E-4	2.3636582811949712E-4
11	0.0	0.0	0.0	1.1818291405974856E-4	2.3636582811949712E-4
12	0.0	0.0	0.0	1.1818291405974856E-4	2.3636582811949712E-4
13	0.0	0.0	0.0	1.1818291405974856E-4	2.3636582811949712E-4
14	0.0	0.0	0.0	1.1818291405974856E-4	2.3636582811949712E-4
15	0.0	0.0	0.0	1.1818291405974856E-4	3.545487421792457E-4
16	0.0	0.0	0.0	1.1818291405974856E-4	3.545487421792457E-4
17	0.0	0.0	0.0	2.3636582811949712E-4	3.545487421792457E-4
18	0.0	0.0	0.0	2.3636582811949712E-4	3.545487421792457E-4
19	0.0	0.0	0.0	2.3636582811949712E-4	3.545487421792457E-4
20	0.0	0.0	0.0	3.545487421792457E-4	3.545487421792457E-4
21	0.0	0.0	0.0	5.909145702987428E-4	4.7273165623899424E-4
22	1.1818291405974856E-4	0.0	0.0	0.001063646226537737	5.909145702987428E-4
23	1.1818291405974856E-4	0.0	0.0	0.0015363778827767313	8.272803984182399E-4
24	1.1818291405974856E-4	0.0	0.0	0.002363658281194971	8.272803984182399E-4
25	1.1818291405974856E-4	0.0	0.0	0.002718207023374217	8.272803984182399E-4
26	1.1818291405974856E-4	0.0	0.0	0.0034273045077327082	8.272803984182399E-4
27	1.1818291405974856E-4	0.0	0.0	0.011345559749735862	8.272803984182399E-4
28	1.1818291405974856E-4	0.0	0.0	0.04242766614744973	8.272803984182399E-4
29	1.1818291405974856E-4	0.0	0.0	0.09017356342758814	9.454633124779885E-4
30	1.1818291405974856E-4	0.0	0.0	0.1538741541057926	9.454633124779885E-4
31	1.1818291405974856E-4	0.0	0.0	0.4125765529825822	9.454633124779885E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTAGGT	45	1.3228091E-4	24.666666	3
GTCTTAC	70	1.9216714E-7	23.785713	1
TAATATA	50	2.7012863E-4	22.199999	4
CTTATAC	425	0.0	19.15294	37
ACTAGAC	60	9.234068E-4	18.5	3
AATATAG	50	0.0070333867	18.499998	5
CGTAGTC	50	0.0070333867	18.499998	14
TAATCAG	65	0.0015794686	17.076923	5
CTAGAAC	65	0.0015794686	17.076923	3
GTATTAT	65	0.0015794686	17.076923	1
GTATTAG	65	0.0015794686	17.076923	1
TACTTAC	165	5.4569682E-11	16.818182	31
GTTCTAG	100	5.8741043E-6	16.65	1
TCGTTAC	260	0.0	15.653846	23
ATAGGAC	120	1.9339332E-6	15.416667	3
ACTAATG	75	0.0041032154	14.8	3
CTACACT	225	1.8189894E-12	14.8	4
AGCCTAC	75	0.0041032154	14.8	1
ATTATGC	75	0.0041032154	14.8	3
CGTTACG	240	0.0	14.645834	24
>>END_MODULE