FastQCFastQC Report
Thu 9 Feb 2017
SRR2727559.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727559.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1921771
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC13350.023.55805237
GTACGAC555.1448226E-420.18181811
TTAGCGC759.271087E-619.7333344
TACTTAC5950.016.16806631
TATAAGG2250.015.6222222
GTACTTA6400.015.60937630
GTAATAC1101.452799E-515.1363643
AGTGTAC7650.014.993463527
TCTTATA20950.014.92362736
ATTATAC1508.1094186E-814.83
CTAGTAC1252.9610674E-614.7999993
TAGACAG4050.014.1604945
TAAGACT2500.014.0599994
CCCGATT1203.302627E-513.87500112
CTCGTTA5800.013.71551822
GTATTAT1356.571994E-613.7037041
CTAGGAC2457.2759576E-1213.5918383
TCGTTAC5150.013.29126323
CGACTTA1951.0270014E-813.28205219
TTTATAC1551.8891988E-613.1290323
Produced by FastQC (version 0.11.5)