FastQCFastQC Report
Thu 9 Feb 2017
SRR2727558.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727558.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1441756
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAC358.870496E-426.428573
TATACTG1550.020.2903235
CTTATAC6600.019.62121437
GTATTAG1653.6379788E-1217.9393961
TCTTATA12250.016.76326637
CGTTACA1352.2204404E-816.44444524
TAGTACT1352.2204404E-816.4444454
TACTTAC2400.016.187531
TTATACT1504.6729838E-916.0333334
GTGCGTT700.002592923115.85714211
GTACTTA2800.015.19642830
GGGTATA1101.4522986E-515.1363641
TCGTTAC4450.014.96629323
TAGACTC1001.0936698E-414.85
GTATATA1001.0936698E-414.81
TTACGGG1508.104689E-814.826
CTCGTTA4600.014.47826122
TTAGACT1152.2105049E-514.4782614
CGGTAGG908.275815E-414.3888881
CTAGGGT1803.3305696E-914.3888884
Produced by FastQC (version 0.11.5)