##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727558.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1441756
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.138914629105063	31.0	31.0	33.0	28.0	34.0
2	31.317197223385925	31.0	31.0	34.0	28.0	34.0
3	31.412825748601012	31.0	31.0	34.0	28.0	34.0
4	35.25917977799295	37.0	35.0	37.0	33.0	37.0
5	34.23965358909552	35.0	35.0	37.0	30.0	37.0
6	34.182991435444	35.0	35.0	37.0	30.0	37.0
7	34.183812656233094	35.0	35.0	37.0	30.0	37.0
8	34.08580647488202	35.0	33.0	37.0	30.0	37.0
9	35.6678890186689	37.0	35.0	39.0	30.0	39.0
10	35.359818859779324	37.0	34.0	39.0	30.0	39.0
11	35.54397137934574	37.0	35.0	39.0	30.0	39.0
12	35.47119068691235	37.0	35.0	39.0	30.0	39.0
13	35.6139936299901	37.0	35.0	39.0	30.0	39.0
14	36.54569358476746	38.0	36.0	40.0	31.0	41.0
15	36.47824874666726	38.0	35.0	40.0	31.0	41.0
16	36.437692647022104	38.0	35.0	40.0	31.0	41.0
17	36.42709445981151	38.0	35.0	40.0	31.0	41.0
18	36.35945402689498	38.0	35.0	40.0	30.0	41.0
19	36.410984937811946	38.0	35.0	40.0	30.0	41.0
20	36.386717308615324	38.0	35.0	40.0	30.0	41.0
21	36.32824070092304	38.0	35.0	40.0	30.0	41.0
22	36.310519949284064	38.0	35.0	40.0	30.0	41.0
23	36.217069323796814	38.0	35.0	40.0	30.0	41.0
24	36.12835458981964	38.0	34.0	40.0	30.0	41.0
25	36.01281700925816	38.0	34.0	40.0	29.0	41.0
26	35.804041737991724	38.0	34.0	40.0	29.0	41.0
27	35.685496020131005	38.0	34.0	40.0	28.0	41.0
28	35.55402301082846	38.0	34.0	40.0	27.0	41.0
29	35.39937687098233	38.0	34.0	40.0	27.0	41.0
30	35.29000260793089	38.0	34.0	40.0	27.0	41.0
31	35.17731571777749	38.0	34.0	40.0	27.0	40.0
32	35.03396205738003	38.0	33.0	40.0	26.0	40.0
33	34.960378871320806	38.0	33.0	40.0	26.0	40.0
34	34.867162682173685	37.0	33.0	40.0	26.0	40.0
35	34.8644299035343	37.0	33.0	39.0	26.0	40.0
36	34.753262687999914	37.0	33.0	39.0	26.0	40.0
37	34.707918676946726	37.0	33.0	39.0	26.0	40.0
38	34.65631909976445	37.0	33.0	39.0	25.0	40.0
39	34.57593517904555	37.0	33.0	39.0	25.0	40.0
40	34.4963260080069	37.0	33.0	39.0	25.0	40.0
41	34.434675492940556	37.0	33.0	39.0	25.0	40.0
42	34.382991990322914	37.0	33.0	39.0	25.0	40.0
43	33.170556599036175	36.0	31.0	39.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	6.0
11	4.0
12	3.0
13	5.0
14	3.0
15	16.0
16	70.0
17	125.0
18	255.0
19	497.0
20	917.0
21	1665.0
22	2709.0
23	4316.0
24	6697.0
25	9852.0
26	14336.0
27	20085.0
28	27683.0
29	37118.0
30	49478.0
31	64633.0
32	83002.0
33	108050.0
34	139250.0
35	175997.0
36	210840.0
37	225401.0
38	190595.0
39	68147.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.56053035326366	17.77277153693135	12.296394119393296	27.370303990411692
2	19.444552337566133	20.325908128698615	34.116105637847184	26.113433895888072
3	20.807404304195718	21.857651364031085	28.90197786588022	28.432966465892978
4	16.343819619963433	14.98075957374202	32.64553780251305	36.0298830037815
5	16.35131048526935	35.183415224212695	32.85202211747341	15.613252173044536
6	37.33329356701134	33.010509406584745	14.365052061513875	15.291144964890035
7	31.1311345331665	29.524760084230618	19.97529401646326	19.368811366139624
8	28.201859399232603	34.365731788180526	18.666681463437644	18.765727349149234
9	28.671356318267442	13.850679310507463	17.164416170281243	40.31354820094385
10	16.991918188653283	26.185360074797675	32.29450753109403	24.528214205455015
11	38.42820837922644	20.479054708286284	19.889703944356743	21.20303296813053
12	22.773201568087803	23.287158159910554	29.06178299240648	24.877857279595162
13	32.32863258415432	18.643723348472278	22.76203462999287	26.265609437380526
14	23.62105654493548	18.756155687925002	23.86007063608544	33.762717131054075
15	26.88693509858811	26.00793754282972	20.89112165997575	26.214005698606424
16	26.01105873670718	24.774233642863287	23.096002374881742	26.11870524554779
17	25.88177195031614	24.457675223824282	23.64040794697577	26.020144878883805
18	25.795696359162022	24.588973446269687	24.206106997300513	25.409223197267778
19	27.177552928512178	24.099015367371454	23.81138001159697	24.912051692519398
20	26.144715194526675	23.707756374865095	23.97943896193253	26.1680894686757
21	25.940519755076448	24.320897572127322	24.45150219593329	25.28708047686294
22	26.56864268295051	23.553083878270662	23.460141660586117	26.418131778192706
23	25.87303260745924	23.845713144249096	24.11205502179287	26.169199226498797
24	25.722729782293257	23.69957191091974	24.096726491861315	26.480971814925685
25	26.333443384317455	23.317260340862113	23.784815183706538	26.56448109111389
26	25.89169041086009	23.759984352414694	24.252578106142785	26.09574713058243
27	26.623575695193917	24.099362166691172	23.515144032693467	25.761918105421444
28	26.149847824458504	24.66249490205	23.022411559237487	26.16524571425401
29	26.149847824458504	24.570385002732777	23.767128418400894	25.512638754407817
30	26.82021090947428	24.009402423156207	23.36713008303763	25.803256584331884
31	26.16434403602274	23.856394563296423	24.023205036081002	25.95605636459983
32	25.77329312310821	24.07952524560328	23.892531052411087	26.254650578877424
33	25.87650060065642	23.60115026398364	23.96230707553844	26.560042059821498
34	26.264083520373767	23.805969942209362	24.573298117018414	25.356648420398457
35	26.259575129217428	23.566817131331515	24.12897882859513	26.044628910855927
36	25.901331431948265	23.318023299365496	24.59972422518096	26.18092104350528
37	25.333343506113376	23.158426252431063	25.0687356251682	26.439494616287362
38	25.92082155371644	22.73741187829286	24.90393658843799	26.437829979552713
39	25.026634187754375	23.108279070799775	25.710799885694946	26.154286855750904
40	26.033670052352825	22.263614647693508	25.837659076847956	25.865056223105714
41	25.014218772108453	22.7369957191092	25.958969478885468	26.289816029896873
42	24.634057357833086	22.764254145639068	26.293700182277725	26.307988314250125
43	24.284830442876604	22.32902099939241	26.084510832623554	27.301637725107437
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	26.0
1	27.5
2	29.0
3	72.5
4	116.0
5	116.0
6	151.0
7	186.0
8	187.0
9	188.0
10	255.5
11	323.0
12	323.0
13	507.5
14	692.0
15	987.5
16	1283.0
17	1367.5
18	1452.0
19	1452.0
20	1831.0
21	2210.0
22	2612.5
23	3015.0
24	3960.0
25	4905.0
26	4905.0
27	6469.0
28	8033.0
29	10027.5
30	12022.0
31	15126.5
32	18231.0
33	18231.0
34	22396.5
35	26562.0
36	31843.0
37	37124.0
38	43881.0
39	50638.0
40	50638.0
41	58819.0
42	67000.0
43	76114.0
44	85228.0
45	97176.5
46	109125.0
47	109125.0
48	121248.5
49	133372.0
50	141148.0
51	148924.0
52	150027.5
53	151131.0
54	151131.0
55	144309.0
56	137487.0
57	128171.0
58	118855.0
59	110693.0
60	102531.0
61	102531.0
62	89937.0
63	77343.0
64	65503.0
65	53663.0
66	44603.5
67	35544.0
68	35544.0
69	29114.0
70	22684.0
71	18067.5
72	13451.0
73	10722.5
74	7994.0
75	7994.0
76	6422.5
77	4851.0
78	3859.0
79	2867.0
80	2178.0
81	1489.0
82	1489.0
83	1080.0
84	671.0
85	499.5
86	328.0
87	229.0
88	130.0
89	130.0
90	82.0
91	34.0
92	22.5
93	11.0
94	8.5
95	6.0
96	6.0
97	4.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1441756.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	69.02668288482973
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.14777650208399	58.774685669577075
2	8.09006459631142	11.168606468147528
3	2.6225933486223796	5.430867582336622
4	1.2533623838540755	3.46061791240278
5	0.759514238212253	2.6213374233795106
6	0.4964111929728038	2.0559370798688246
7	0.32732078005949683	1.581570738074739
8	0.23647553482618044	1.3058497401973814
9	0.17075559984967512	1.0608023377469162
>10	0.8576776534479855	9.825722820184325
>50	0.027069264312475028	1.263371787944022
>100	0.01047531630228029	1.2440105317822372
>500	5.035891450028999E-4	0.20661990835803024
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	1.3871972788738178E-4	0.0
6	0.0	0.0	0.0	1.3871972788738178E-4	1.3871972788738178E-4
7	0.0	0.0	0.0	1.3871972788738178E-4	1.3871972788738178E-4
8	0.0	0.0	0.0	1.3871972788738178E-4	2.0807959183107266E-4
9	0.0	0.0	0.0	1.3871972788738178E-4	2.7743945577476356E-4
10	0.0	0.0	0.0	2.0807959183107266E-4	2.7743945577476356E-4
11	0.0	0.0	0.0	2.0807959183107266E-4	2.7743945577476356E-4
12	0.0	0.0	0.0	2.0807959183107266E-4	2.7743945577476356E-4
13	0.0	0.0	0.0	2.0807959183107266E-4	2.7743945577476356E-4
14	0.0	0.0	0.0	2.0807959183107266E-4	2.7743945577476356E-4
15	0.0	0.0	0.0	2.0807959183107266E-4	3.467993197184544E-4
16	0.0	0.0	0.0	2.7743945577476356E-4	3.467993197184544E-4
17	0.0	0.0	0.0	4.855190476058362E-4	3.467993197184544E-4
18	0.0	0.0	0.0	5.548789115495271E-4	3.467993197184544E-4
19	0.0	0.0	0.0	5.548789115495271E-4	3.467993197184544E-4
20	0.0	0.0	0.0	7.629585033805998E-4	3.467993197184544E-4
21	0.0	0.0	0.0	0.001179117687042745	3.467993197184544E-4
22	0.0	0.0	0.0	0.0017339965985922721	3.467993197184544E-4
23	0.0	0.0	0.0	0.002080795918310727	4.855190476058362E-4
24	0.0	0.0	0.0	0.003190553741409781	4.855190476058362E-4
25	0.0	0.0	0.0	0.0035373530611282353	4.855190476058362E-4
26	0.0	0.0	0.0	0.00471647074817098	4.855190476058362E-4
27	0.0	0.0	0.0	0.012276695918033287	4.855190476058362E-4
28	0.0	0.0	0.0	0.03814792516902999	4.855190476058362E-4
29	0.0	0.0	0.0	0.07948640407946976	4.855190476058362E-4
30	0.0	0.0	0.0	0.13684701156090212	4.855190476058362E-4
31	0.0	0.0	0.0	0.40284208978495667	4.855190476058362E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATAC	35	8.870496E-4	26.42857	3
TATACTG	155	0.0	20.290323	5
CTTATAC	660	0.0	19.621214	37
GTATTAG	165	3.6379788E-12	17.939396	1
TCTTATA	1225	0.0	16.763266	37
CGTTACA	135	2.2204404E-8	16.444445	24
TAGTACT	135	2.2204404E-8	16.444445	4
TACTTAC	240	0.0	16.1875	31
TTATACT	150	4.6729838E-9	16.033333	4
GTGCGTT	70	0.0025929231	15.857142	11
GTACTTA	280	0.0	15.196428	30
GGGTATA	110	1.4522986E-5	15.136364	1
TCGTTAC	445	0.0	14.966293	23
TAGACTC	100	1.0936698E-4	14.8	5
GTATATA	100	1.0936698E-4	14.8	1
TTACGGG	150	8.104689E-8	14.8	26
CTCGTTA	460	0.0	14.478261	22
TTAGACT	115	2.2105049E-5	14.478261	4
CGGTAGG	90	8.275815E-4	14.388888	1
CTAGGGT	180	3.3305696E-9	14.388888	4
>>END_MODULE