FastQCFastQC Report
Thu 9 Feb 2017
SRR2727557.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727557.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1149371
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCAAATCCCAGCAACCACATGGTGGCTCACAACCATCCGTA13470.11719453509789267No Hit
GTTGTGAGCCACCATGTGGTTGCTGGGATTTGAACTCTGGACC13290.11562846113221928No Hit
GGTCCAGAGTTCAAATCCCAGCAACCACATGGTGGCTCACAAC13230.1151064364769948No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA3900.025.1410261
TATACGG400.001931187123.1255
TACTTAC4950.020.55555531
ATACCGT450.003825808420.5555556
GTACTTA5650.019.31858430
CTTATAC6900.019.30434837
TTAACAC701.219197E-418.53
ACATATA2200.018.536
CCTAATA500.00703488418.4999982
TACATAT2700.017.81481435
TTACATA2750.017.4909134
ATACTAT752.067266E-417.2666666
CATATAA2150.017.20930337
TCTTATA11450.016.4803537
ACTTACA4400.015.97727332
ACTAGAC1059.343015E-615.8571443
ACTATAG1403.4726327E-815.8571432
TATAAGT957.059676E-515.5789484
ATAGAAC855.364151E-415.2352943
GTGTACT7300.015.2054828
Produced by FastQC (version 0.11.5)